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1.
L. Van Camphnhout D. Iserentant H. Verachtert 《Journal of the Institute of Brewing》1998,104(4):197-202
During germination, the metabolic activity of sterile barley was more pronounced than the activity of barley contaminated by its endogenous microbial population. CO2 and heat production profiles showed higher peak values when microbial activity was eliminated. Moreover, the maxima in the profiles appeared sooner than with barley in the presence of its natural microflora. More intensive growth of the acrospire was observed with sterile barley and a malt was obtained with a higher and more homogeneous modification. Reverse effects were noticed when sterile barley was inoculated with an Erwinia sp. strain and germination was evaluated. The results provide clear evidence for the strong interaction between microbial and plant metabolism, even for non dormant and non water sensitive barley. The ratio of CO2 production to heat production levels was a useful tool for on-line monitoring of microbial activity during germination. In this way, the effects of the microbial activity can be taken into account in the development of a more effective control of the malting process. All experiments were performed using a specially designed solid-state reactor. 相似文献
2.
Effects of allelochemicals on plant respiration and oxygen isotope fractionation by the alternative oxidase 总被引:8,自引:1,他引:8
The goal of this investigation was to determine the effects of allelochemicals on plant respiration that thereby may be responsible for their role in growth inhibition. We have tested the effects of juglone, quercetin, cinnamic acid, and-pinene on respiration rates, and electron partitioning through the cytochrome and alternative respiratory pathways, by measuring on-line oxygen consumption and oxygen isotope fractionation in soybean cotyledon tissue. Cinnamic acid and-pinene decreased the oxygen consumption rate and increased the relative partitioning of electron transport to the alternative pathway. Possible biochemical mechanisms of these effects are discussed. 相似文献
3.
4.
Studies suggest that oligotrophic lakes are net heterotrophic and act as net sources of CO2, whereas eutrophic lakes are net autotrophic and act as net CO2 sinks. Data on plankton community metabolism in Lake Taihu contradict this hypothesis. Here, the ratios of depth integrated gross primary production (GPP) to plankton community respiration (PCR) were less than one on 75% of the study sampling dates, indicating that this system was net heterotrophic. Partial pressure estimated for CO2 also indicated that the lake was a net source of CO2. Net heterotrophic conditions here may be related to limitation of phytoplankton photosynthesis by the poor underwater light climate (due to elevated suspended solids (SS) and nutrients originating in the catchment) and the preferential enhancement of respiration by high water temperatures. GPP and PCR were significantly correlated (PCR = 1.22GPP + 0.46, r2 = 0.80) indicating a partial dependence of heterotrophs on algal derived carbon. The slope of the regression line relating PCR to GPP was more similar to slopes found in rivers than in lakes, likely due to the large nutrient and SS load to the lake. 相似文献
5.
Chaima Chammakhi Alexandre Boscari Marie Pacoud Grgoire Aubert Haythem Mhadhbi Renaud Brouquisse 《International journal of molecular sciences》2022,23(21)
Drought is an environmental stress that strongly impacts plants. It affects all stages of growth and induces profound disturbances that influence all cellular functions. Legumes can establish a symbiosis with Rhizobium-type bacteria, whose function is to fix atmospheric nitrogen in organs called nodules and to meet plant nitrogen needs. Symbiotic nitrogen fixation (SNF) is particularly sensitive to drought. We raised the hypothesis that, in drought-stressed nodules, SNF inhibition is partly correlated to hypoxia resulting from nodule structure compaction and an increased O2 diffusion barrier, and that the nodule energy regeneration involves phytoglobin–nitric oxide (Pgb–NO) respiration. To test this hypothesis, we subjected faba bean (Vicia faba L.) plants nodulated with a Rhizobium laguerreae strain to either drought or osmotic stress. We monitored the N2-fixation activity, the energy state (ATP/ADP ratio), the expression of hypoxia marker genes (alcohol dehydrogenase and alanine aminotransferase), and the functioning of the Pgb–NO respiration in the nodules. The collected data confirmed our hypothesis and showed that (1) drought-stressed nodules were subject to more intense hypoxia than control nodules and (2) NO production increased and contributed via Pgb–NO respiration to the maintenance of the energy state of drought-stressed nodules. 相似文献
6.
Han-Yu Wu Qing-Qing Zou Wen-Tao Ji Ying-Wei Wang Wang-Feng Zhang Chuang-Dao Jiang 《International journal of molecular sciences》2022,23(23)
A leaf structure with high porosity is beneficial for lateral CO2 diffusion inside the leaves. However, the leaf structure of maize is compact, and it has long been considered that lateral CO2 diffusion is restricted. Moreover, lateral CO2 diffusion is closely related to CO2 pressure differences (ΔCO2). Therefore, we speculated that enlarging the ΔCO2 between the adjacent regions inside maize leaves may result in lateral diffusion when the diffusion resistance is kept constant. Thus, the leaf structure and gas exchange of maize (C4), cotton (C3), and other species were explored. The results showed that maize and sorghum leaves had a lower mesophyll porosity than cotton and cucumber leaves. Similar to cotton, the local photosynthetic induction resulted in an increase in the ΔCO2 between the local illuminated and the adjacent unilluminated regions, which significantly reduced the respiration rate of the adjacent unilluminated region. Further analysis showed that when the adjacent region in the maize leaves was maintained under a steady high light, the photosynthesis induction in the local regions not only gradually reduced the ΔCO2 between them but also progressively increased the steady photosynthetic rate in the adjacent region. Under field conditions, the ΔCO2, respiration, and photosynthetic rate of the adjacent region were also markedly changed by fluctuating light in local regions in the maize leaves. Consequently, we proposed that enlarging the ΔCO2 between the adjacent regions inside the maize leaves results in the lateral CO2 diffusion and supports photosynthesis in adjacent regions to a certain extent under fluctuating light. 相似文献
7.
Kattri-Liis Eskla Hans Vellama Liisi Tarve Hillar Eichelmann Toomas Jagome Rando Porosk Vello Oja Heikko Rmma Nadeda Peet Agu Laisk Vallo Volke Eero Vasar Hendrik Luuk 《International journal of molecular sciences》2022,23(17)
Ischemia reperfusion injury is common in transplantation. Previous studies have shown that cooling can protect against hypoxic injury. To date, the protective effects of hypothermia have been largely associated with metabolic suppression. Since kidney transplantation is one of the most common organ transplant surgeries, we used human-derived renal proximal tubular cells (HKC8 cell line) as a model of normal renal cells. We performed a temperature titration curve from 37 °C to 22 °C and evaluated cellular respiration and molecular mechanisms that can counteract the build-up of reducing equivalents in hypoxic conditions. We show that the protective effects of hypothermia are likely to stem both from metabolic suppression (inhibitory component) and augmentation of stress tolerance (activating component), with the highest overlap between activating and suppressing mechanisms emerging in the window of mild hypothermia (32 °C). Hypothermia decreased hypoxia-induced rise in the extracellular lactate:pyruvate ratio, increased ATP/ADP ratio and mitochondrial content, normalized lipid content, and improved the recovery of respiration after anoxia. Importantly, it was observed that in contrast to mild hypothermia, moderate and deep hypothermia interfere with HIF1 (hypoxia inducible factor 1)-dependent HRE (hypoxia response element) induction in hypoxia. This work also demonstrates that hypothermia alleviates reductive stress, a conceptually novel and largely overlooked phenomenon at the root of ischemia reperfusion injury. 相似文献
8.
Irina V. Ogneva Yulia S. Zhdankina Oleg V. Kotov 《International journal of molecular sciences》2022,23(14)
Studies of reproductive function under long-term space flight conditions are of interest in planning the exploration of deep space. Motility, including the use of various inhibitors, cellular respiration, and the content of cytoskeletal proteins were studied, assessing the level of expression of the corresponding genes in spermatozoa of Drosophila melanogaster, which were in space flight conditions for 12 days. The experiment was carried out twice on board the Russian Segment of the International Space Station. Sperm motility speed after space flight, and subsequently 16 h after landing, is reduced relative to the control by 20% (p < 0.05). In comparison with the simulation experiment, we showed that this occurs as a result of the action of overloads and readaptation to the Earth’s gravity. At the same time, cellular respiration, the content of proteins of the respiratory chain, and the expression of their genes do not change. We used kinase inhibitor 6-(dimethylamino)purine (6-DMAP) and phosphatase inhibitors; 6-DMAP restored the reduced the speed of spermatozoa in the flight group to that of the control. These results can be useful in developing a strategy for protecting reproductive health during the development of other bodies in the solar system. 相似文献
9.
Alexander V. Panov Vladimir I. Mayorov Anna E. Dikalova Sergey I. Dikalov 《International journal of molecular sciences》2023,24(1)
Scientists have long established that fatty acids are the primary substrates for kidney mitochondria. However, to date we still do not know how long-chain and middle-chain fatty acids are oxidized at the mitochondrial level. Our previous research has shown that mitochondria from the heart, brain, and kidney oxidize palmitoylcarnitine at a high rate only in the presence of succinate, glutamate, or pyruvate. In this paper, we report properties of the isolated kidney mitochondria and how malate and succinate affect the oxidation of C16 and C8 acylcarnitines. The isolated kidney mitochondria contain very few endogenous substrates and require malate to oxidize pyruvate, glutamate, and C16 or C8 acylcarnitines. We discovered that with 10 µM of C16 or C8 acylcarnitines, low concentrations of malate (0.2 mM) or succinate (0.5 mM) enhance the States 4 and 3 respiratory rates several times. The highest respiration rates were observed with C16 or C8 acylcarnitines and 5 mM succinate mixtures. Results show that kidney mitochondria, unlike the heart and brain mitochondria, lack the intrinsic inhibition of succinate dehydrogenase. Additionally, results show that the oxidation of fatty acid by the small respirasome’s supercomplex generates a high level of CoQH2, and this makes SDH in the presence of succinate reverse the flow of electrons from CoQH2 to reduce fumarate to succinate. Finally, we report evidence that succinate dehydrogenase is a key mitochondrial enzyme that allows fast oxidation of fatty acids and turns the TCA cycle function from the catabolic to the anabolic and anaplerotic metabolic pathways. 相似文献
10.