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以超氧化物歧化酶(SOD)为模板蛋白,研究其分离-修饰耦合工艺.即将SOD选择性地吸附在铜离子螯合葡聚糖凝胶层析柱上,先不经洗脱分离,直接用活化的聚乙二醇(PEG)5000对吸附的SOD进行化学修饰,再通过特异性洗脱分离获得PEG-SOD,使得SOD分离纯化和修饰过程同步完成.获得了一条SOD金属螯合层析分离-PEG化学修饰耦合工艺路线.获得的PEG-SOD比活9055.1 U/mg,纯化倍数1.75倍,氨基修饰率42.3%,修饰前后活性保留87.1%.证明SOD的分离-修饰耦合工艺是可行的. 相似文献
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采煤沉陷地地形数据是损毁评价、土地复垦的基础。本文以山东省邱集煤矿采煤沉陷地为研究区,设计了12种飞行方案,通过分析平面精度、高程精度、时间成本等因素,探究将消费级无人机应用于采煤沉陷地地形数据采集的最佳飞行方案。研究表明:增加控制点的个数和像片重叠度可以有效提高消费级无人机航测影像的精度,但当重叠度增大到一定程度时,精度提高的幅度趋于稳定;12种飞行方案获得地形图的平面精度均满足1∶500的精度要求,高程精度满足1∶1 000的精度要求,其中布设9个像控点、重叠度为9070或9080时高程精度最高;在120m的航高下,布设9个像控点,将重叠度设置为9070时航测摄影像精度最高;当飞行面积较大时,考虑时间成本,重叠度设置为8070性价比较优。 相似文献
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The equilibrium reconstruction is important to study the tokamak plasma physical processes.To analyze the contribution of fast ions to the equilibrium,the kinetic equilibria at two time-slices in a typical H-mode discharge with different auxiliary heatings are reconstructed by using magnetic diagnostics,kinetic diagnostics and TRANSP code.It is found that the fast-ion pressure might be up to one-third of the plasma pressure and the contribution is mainly in the core plasma due to the neutral beam injection power is primarily deposited in the core region.The fast-ion current contributes mainly in the core region while contributes little to the pedestal current.A steep pressure gradient in the pedestal is observed which gives rise to a strong edge current.It is proved that the fast ion effects cannot be ignored and should be considered in the future study of EAST. 相似文献
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