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1.
Background: Bkv-miR-B1-5p is a viral micro-RNA (miRNA) specifically produced during BK polyomavirus (BKPyV) replication. Recent studies have suggested using bkv-miR-B1-5p as a biomarker to monitor viral infection and predict complications in kidney transplant patients. To identify the technical limitations of this miRNA quantification in biological samples, knowledge of its stability and distribution in the extracellular compartment is necessary. Moreover, a proof of concept for using bkv-miR-B1-5p as a biomarker of active replication in chronic infection is still missing in the published literature. Methods: The stability of bkv-miR-B1-5p was evaluated in samples derived from cell cultures and in urine from BKPyV-infected kidney transplant recipients. The miRNA was quantified in different fractions of the extracellular compartment, including exosomes, and protein binding was evaluated. Finally, we developed an in vitro model for chronic culture of BKPyV clinical isolates to observe changes in the bkv-miR-B1-5p level during persistent infections. Results: Bkv-miR-B1-5p is a stable biomarker in samples from humans and in vitro experiments. Marginally associated with the exosomes, most of the circulating bkv-miR-B1-5p is bound to proteins, especially Ago2, so the miRNA quantification does not require specific exosome isolation. The bkv-miR-B1-5p level is predictable of viral infectivity, which makes it a potential specific biomarker of active BKPyV replication after kidney transplantation.  相似文献   
2.
云南某铜矿地处高海拔地区,投产后经多方比对,采用以国外进口原料为主要成分的起泡剂PL-1,投产前两年原矿性质简单,含泥量少,取得的生产指标较好;随着开采的深入,矿石含泥量增多,继续使用原有起泡剂,生产指标下降幅度较大。为了提高生产指标并实现起泡剂国产化替代,进行了新型特效起泡剂BK208的工业应用试验,经过一段时间的工业试验对比,采用原起泡剂PL-1工业试验累计指标:铜精矿含Cu 23.93%、回收率84.67%;采用起泡剂BK208的累计指标:铜精矿含Cu 22.67%、回收率86.50%。采用起泡剂BK208可比采用原用起泡剂PL-1的铜回收率提高了1.83个百分点。预估每年可增经济收益超6 800万元。  相似文献   
3.
黑龙江某钼矿钼原矿品位为0.108%,实验室采用"一段粗磨浮选抛尾,钼粗精矿两段再磨再选"选矿工艺流程,获得了较好的指标,钼精矿品位50.57%,回收率89.38%。试验研究为开发利用该类型钼矿资源,提供了技术依据。  相似文献   
4.
BK992萃取除铜净化硫酸镍钴溶液   总被引:8,自引:1,他引:8  
以BK992为萃取剂,从废镍合金硫酸溶液中萃取分离铜,研究了pH值、相比(O/A)、BK992浓度和萃取时间对萃取的影响,确定了BK992萃取铜的优化条件.结果表明:BK992的萃铜速度很快,随着pH值升高,铜萃取率增大,但当pH大于4.0时,镍钴损失较大;相比越大,萃取分离效果越好;BK992浓度的影响也较大,随着BK992浓度增加,铜分配比增大.室温下BK992萃取铜的最佳工艺条件:BK992浓度为20%,相比O/A为1:2,水相初始pH为3.0,萃取时间为10 min.在优化条件下,待处理液的一级萃取率达93.06%,20%BK992萃铜的饱和容量为17.30 g/L;一级反萃率达91.79%,铜净传递量为15.88 g/L.  相似文献   
5.
The objective of this study was to develop a specific visual dataset comprising black‐appearing samples with low lightness (L* ranging from approximately 10.4 to 19.5), varying in hue and chroma, evaluating their visual differences against a reference sample, and testing the performance of major color difference formulas currently in use as well as OSA‐UCS‐based models and more recent CAM02 color difference formulas including CAM02‐SCD and CAM02‐UCS models. The dataset comprised 50 dyed black fabric samples of similar structure, and a standard (L*= 15.33, a* = 0.14, b* = ?0.82), with a distribution of small color differences, in ΔE*ab, from 0 to approximately 5. The visual color difference between each sample and the standard was assessed by 19 observers in three separate sittings with an interval of at least 24 hours between trials using an AATCC standard gray scale for color change, and a total of 2850 assessments were obtained. A third‐degree polynomial equation was used to convert gray scale ratings to visual differences. The Standard Residual Sum of Squares index (STRESS) and Pearson's correlation coefficient (r), were used to evaluate the performance of various color difference formulae based on visual results. According to the analysis of STRESS index and correlation coefficient results CAM02 color difference equations exhibited the best agreement against visual data with statistically significant improvement over other models tested. The CIEDE2000 (1:1:1) equation also showed good performance in this region of the color space. © 2013 Wiley Periodicals, Inc. Col Res Appl, 39, 589–598, 2014  相似文献   
6.
BK7 is a high-quality crown glass which is used where additional benefits such as temperature sensitive applications of fused silica glass are not required. Due to very low inclusion content with extremely low bubbles, BK7 glass can find its application in lens manufacturing. The present work focuses on nanofinishing of the BK7 glass specimen for ratifying its utility in practical application. A programmable logic controlled 3-axis motions are fed to the magnetorheological (MR) rotating tool for finishing the glass specimen. MR polishing fluid used for nanofinishing consists of deionized water, magnetic iron particles, and cerium oxide powder. Under the influence of magnetic field, the stiffened MR polishing fluid is assisted in reducing the surface roughness of glass up to nanolevel range. Optical properties such as transmittance, absorbance, and reflectance of finished BK7 glass are analyzed and found suitable for lens manufacturing. Results of higher surface quality with excellent finishing are obtained by the present MR finishing process. After 90?min of finishing, the surface roughness values Ra and Rq are reduced to 17 and 27?nm from the initial values of 41 and 57?nm, respectively. To study the surface morphology, scanning electron microscopy is performed on BK7 glass.  相似文献   
7.
内蒙古某多金属矿尾矿含CaF216.67%、SiO2 32.84%、CaCO3 8.52%,属于典型的高硅高碳酸钙难处理萤石矿。本研究针对该尾矿,采用碳酸钠为活化剂、水玻璃为抑制剂、BK410为捕收剂,进行了萤石综合回收的试验研究。最后采用一次粗选、一次扫选和十次精选——中矿单独处理工艺流程,可以获得含CaF2 95.10%、回收率46.97%的高品位萤石精矿Ⅰ和含CaF2 85.35%,回收率10.12%的萤石精矿Ⅱ。  相似文献   
8.
陕西震奥鼎盛矿业有限公司铅锌选厂在铅锌矿浮选生产原工艺以乙硫氮、丁基黄药作为捕收剂,原药剂制度下铅精矿回收率偏低,锌精矿回收率波动大,铅锌互含率偏高,且石灰用量大,影响回水质量,并污染环境。自2011年起,原矿品位下降,黄铁矿含量上升,为了抑制黄铁矿上浮,不得不加大石灰用量。针对以上问题,企业将方铅矿捕收剂调整为弱碱性下捕收效果良好的BK906,闪锌矿捕收剂调整为MB。一年来的生产结果表明,BK906与MB配合使用,能在原矿品位下降的情况下,有效提高铅与锌的回收率,降低铅锌互含率,石灰用量由过去的10kg/t降低至5kg/t,具有良好的环保效应。  相似文献   
9.
并行前缀加法器的研究与实现   总被引:5,自引:2,他引:3  
随着微处理器运算速度的大幅度提高,对快速加法器的需求也越来越高.当vLSI工艺进入深亚微米阶段的时候,很多情况下,无论是在面积还是在时序上连线都起着决定性的作用.文章基于不同的CMOS工艺,针对三种不同结构的并行前缀加法器,在不同数据宽度的情况下进行性能比较,根据深亚微米下金属互连线对加法器性能的影响,挑选出适合深亚微米工艺的加法器结构.  相似文献   
10.
某金矿石矿物加工试验研究   总被引:1,自引:1,他引:0  
为了给开发某金矿提供依据,在工艺矿物学研究的基础上,对该矿石进行了可选性试验研究。采用异戊基黄药+BK903G为选金捕收剂,完成了两粗、两扫、两精闭路流程试验。对于金品位为2.87 g/t的原矿,在磨矿细度为-0.074 mm占80%的情况下,可获得金品位51.94 g/t、金回收率为82.06%的金精矿。  相似文献   
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