Evaluation of a Novel Plasmid for Simultaneous Gene Electrotransfer-Mediated Silencing of CD105 and CD146 in Combination with Irradiation

Targeting tumor vasculature through specific endothelial cell markers represents a promising approach for cancer treatment. Here our aim was to construct an antibiotic resistance gene-free plasmid encoding shRNAs to simultaneously target two endothelial cell markers, CD105 and CD146, and to test its functionality and therapeutic potential in vitro when delivered by gene electrotransfer (GET) and combined with irradiation (IR). Functionality of the plasmid was evaluated by determining the silencing of the targeted genes using qRT-PCR. Antiproliferative and antiangiogenic effects were determined by the cytotoxicity assay tube formation assay and wound healing assay in murine endothelial cells 2H-11. The functionality of the plasmid construct was also evaluated in malignant melanoma tumor cell line B16F10. Additionally, potential activation of immune response was measured by induction of DNA sensor STING and proinflammatory cytokines by qRT-PCR in endothelial cells 2H-11. We demonstrated that the plasmid construction was successful and can efficiently silence the expression of the two targeted genes. As a consequence of silencing, reduced migration rate and angiogenic potential was confirmed in 2H-11 endothelial cells. Furthermore, induction of DNA sensor STING and proinflammatory cytokines were determined, which could add to the therapeutic effectiveness when used in vivo. To conclude, we successfully constructed a novel plasmid DNA with two shRNAs, which holds a great promise for further in vivo testing.     

淬火温度对G105钢组织和性能的影响

利用硬度计、拉伸试验机、冲击试验机和光学显微镜等手段,研究了G105钢分别在890、910和930 ℃保温150 min淬火,随后进行630 ℃保温180 min回火处理后组织和性能变化。结果表明:随着淬火温度的升高,G105钢淬火硬度越来越高;经回火处理后,淬火温度为890 ℃和910 ℃时,调质硬度无太大差异,分别为33.2 HRC和32.7 HRC,淬火温度为930 ℃的调质硬度相对提高约1.5 HRC。试验钢强度随着淬火温度的升高也呈现升高趋势,但冲击韧性呈先升高后下降的趋势,这主要是由于调质后存在粒状碳化物的析出现象,导致其冲击韧性显著下降,故认为当淬火温度选取910 ℃时,获得的G105钢综合力学性能较佳。     

LLM-105/CL-20基高能钝感PBX的制备与性能表征

为满足含能材料高能钝感的要求,以CL-20为主体炸药,LLM-105为钝感剂,采用溶液水悬浮法制备了LLM-105质量分数分别为10%、20%、30%的3种LLM-105/CL-20基PBX。通过扫描电子显微镜(SEM)、粉末X射线衍射仪(PXRD)和差示扫描量热仪(DSC)对样品的形貌、晶体结构和热性能进行表征,并测试其机械感度;采用EXPLO5软件计算了其爆轰参数。结果表明,LLM-105/CL-20基PBX样品呈类球形,颗粒密实,粒径约为500μm;PBX中各组分的晶体结构未发生改变;3种配方的热安定性都较好,且随着钝感剂LLM-105含量的增加,LLM-105/CL-20基PBX的热爆炸临界温度呈递增趋势;与原料CL-20相比,3种LLM-105/CL-20基PBX的特性落高分别提高了25.88、33.68、37.18 cm,摩擦爆炸概率分别下降29%、38%、45%;LLM-105质量分数为10%的LLM-105/CL-20基PBX的特性落高与PBX-9501相当,而LLM-105质量分数为20%和30%的LLM-105/CL-20基PBX分别比PBX-9501高16.6%和25.12%;理论爆速分别高381.76、279.2、82.03 m/s。3种配方LLM-105/CL-20基PBX炸药的爆轰性能明显优于PBX-9501。     

Endoglin Targeting: Lessons Learned and Questions That Remain

Approximately 30 years ago, endoglin was identified as a transforming growth factor (TGF)-β coreceptor with a crucial role in developmental biology and tumor angiogenesis. Its selectively high expression on tumor vessels and its correlation with poor survival in cancer patients led to the exploration of endoglin as a therapeutic target for cancer. The endoglin neutralizing antibody TRC105 (Carotuximab^®, Tracon Pharmaceuticals (San Diego, CA, USA) was subsequently tested in a wide variety of preclinical cancer models before being tested in phase I-III clinical studies in cancer patients as both a monotherapy and in combination with other chemotherapeutic and anti-angiogenic therapies. The combined data of these studies have revealed new insights into the role of endoglin in angiogenesis and its expression and functional role on other cells in the tumor microenvironment. In this review, we will summarize the preclinical work, clinical trials and biomarker studies of TRC105 and explore what these studies have enabled us to learn and what questions remain unanswered.     

C105管桩混凝土设计参数取值的探讨

通过试验得出了C105混凝土100 mm×100 mm×100 mm立方体非标准试件抗压强度换算系数以及C105混凝土的标准方差和强度变异系数。采用理论计算,得到了棱柱体抗压强度与立方体抗压强度的比值α_c1,并与试验实测平均值进行了比较,验证了理论计算方法的可行性。此外,通过对比分析,探讨了直接外延法和引用公式法两种C105混凝土设计参数计算方法的正确性,研究结果可为C105管桩混凝土的设计和应用提供参考。     

LLM-105的Cu(Ⅱ)和Co(Ⅲ)含能配合物:热分解特性及与HMX相容性

为了研究LLM-105(2,6-二氨基-3,5-二硝基吡嗪-1-氧化物)与过渡金属Cu和Co配合物的热分解特性及其相容性,合成了LLM-105与Cu(Ⅱ)和Co(Ⅲ)的配合物,用傅里叶变换红外光谱和粉末X射线衍射表征其结构。采用差示扫描量热法(DSC)测试了不同升温速率下Cu(Ⅱ)和Co(Ⅲ)配合物的分解温度,并用Kissinger法和Ozawa-Doyle法计算了其表观活化能(E)和指前因子(A);利用转化率(α)和与其对应的温度(T),得到不同转化率下活化能随温度变化曲线。利用DSC测试了Cu(Ⅱ)和Co(Ⅲ)配合物与HMX在不同质量比下混合炸药的分解温度,并对其相容性进行了分析,结果表明,Cu(Ⅱ)配合物与HMX相容性较好,且优于Co(Ⅲ)配合物。     

核电磁脉冲模拟器的分析和设计

从理论上分析了核电磁脉冲中的工作原理,仿真分析了峰化电容器、主开关和引线电感,阻尼电阻和主开关导通时间对输出脉冲前沿和脉宽的影响.实验最后利用峰化电容谐振充电、冷阴极撬棒管导通放电来研制出核电磁脉冲源.在实验工作空间获得前沿为10 ns、脉宽为200 ns、场强达到50kV/m的强电磁环境,适用于GJB151A-97中...     

CZX系列无毒钙锌稳定剂的研制及其应用

PVC稳定剂的无毒化已经是大势所趋。本文概述了CZX系列无毒钙锌稳定剂的研制及其在UL 105℃电缆料中的应用。CZX系列稳定剂是一个全新的无毒稳定剂体系,应用了游离基的中和、吸附等多种理论,针对不同阶段的PVC分解问题,从不同的角度对PVC进行全面稳定化,效果比传统稳定剂更为理想。     

流态化及缓苏干燥技术对泰国香稻的香味和磨粉品质的影响

将高温（〉100。C）流态化干燥和缓苏相结合干燥泰国香稻（KhaoDawkMali105，籼稻），使其湿含量降至13-14％（湿基），以便于安全贮存。研究了干燥与贮存过程对籼米香味和磨粉品质的影响。泰国香稻需要经过两个干燥阶段。第一阶段是在高温流化床中干燥，缓苏30分钟，然后返回高温流化床干燥或阴干，直至湿含量降低到安全贮存水平。实验结果表明，干燥温度对香稻的整精米率（HRY）、白度指数（wI）和2AP含量均有影响。多数干燥温度下整精米率的水平都比较低，但150℃除外，此情况下整精米率（psO．05）显著提高。然而，当一次流态化干燥和缓苏之后，以阴干代替二次流态化干燥，香稻的整精米率显著增加，尤其是在135℃和50℃时（p≤O．05）。在贮存期间，白度指数和2AP舍量显著降低（ps0．05），而整精米率保持不变。对比环境温度（28．30℃）和15℃贮存温度，结果表明保持香米2AP舍量和白度指数的适宜保存温度是15℃。然而贮存条件对整精米率影响不大（p〉0．05）。本研究将有益于泰国香米行业的发展，为泰国香米的干燥起到指导性作用。     

Half-Lives and Gamma-Ray Energies of Short-Lived Molybdenum Isotopes

Short-lived molybdenum isotopes produced by the ²³⁶U(n, f) reaction were isolated by rapid paper electrophoretic technique. Gamma-ray spectra of the isolated molybdenum fraction were measured with high resolution Ge(Li) detector. The half-lives of ¹⁰³Mo (70±1 sec), ¹⁰⁴Mo (66±2 sec) and ¹⁰⁵Mo (30±3 and 40±5 sec) were determined by analyzing the genetic relationships of the known photopeaks of their technetium daughters. Decay analysis of observed photopeaks free from interference resulted in the detection of many new short-lived photopeaks with half-lives of 10–70 sec, and which were not relevant to technetium isotopes. These new photopeaks were ascribed to ^103-106Mo, based on examination of their decay behavior.