A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products

Immunochromatographic assays (ICAs) are considered as a suitable diagnostic tool for the detection of mycotoxins. Mycotoxins and especially, ochratoxin A are analytes with more demanding sensitivity requirements. To enhance the sensitivity of current immunochromatographic assays for ochratoxin A (OTA), a novel sensitive ICA was developed in this study. In the assay, microspheres enclosing fluorescent europium (III) [Eu(III)] nanoparticles (EuNPs) were used as a label for OTA monoclonal antibody (OTA-mAb) conjugation. Accordingly, assay was called time-resolved fluorescent immunochromatographic assay (TRFICA). The test strip was composed of three parts: a sample pad, nitrocellulose membrane and an absorbent pad. As for detection, a proper concentration of conjugated microspheres was pipetted into the microtube and sample extract was added to it. Then the strip was inserted into the tube and the fluid flow along the strip. The TRFICA results were obtained in 8 min and read by a portable TRFICA strip reader. The established method allows quantitative determination of OTA with limit of detection as low as 1.0 μg kg⁻¹ in the samples. For validation, spiked samples including wheat, maize, soybean and rice were respectively assayed by TRFICA and a standard high performance liquid chromatography equipped with a fluorescence detector (HPLC-FLD), and good agreement of results was obtained between two methods.     

液化气钢瓶车载GPS移动配售系统

根据"十二五"上海燃气转型发展新形势,液化石油气用户将呈现分布范围广、不均匀、流动性大的特点,通过国内外液化气行业移动配送系统应用现状与趋势分析,结合上海实际,上海液化石油气经营有限公司开发了基于上海燃气服务平台,利用GPS设备运用于液化气钢瓶车辆配送、销售的集成系统并成功运用,以期对推动行业更及时、准确地实现瓶装液化气全配送服务。     

Daily concentrations of trace metals in aerosols in Beijing, China, determined by using inductively coupled plasma mass spectrometry equipped with laser ablation analysis, and source identification of aerosols

This paper describes the daily concentrations of trace metals and ionic constituents in the aerosol of Beijing, China from March 2001 to August 2003. Daily PM10 concentrations were also measured from September 2001 to August 2003. The daily average PM10 concentration at Beijing, China from September 2001 to August 2003 was 171+/-117 microg m(-3) (n = 673), which is 5-fold higher than at Yokohama, Japan. Trace metal concentrations were analyzed by using inductively coupled plasma mass spectrometry equipped with a laser ablation sample introduction (LA/ICP-MS), which is a rapid and simultaneous method for multi-element analysis. The daily average metal concentrations in TSP in Beijing from March 2001 to August 2003 were: Al: 3.5+/-2.4 (n = 727), Ti: 0.47+/-0.35 (n = 720), V: 0.013+/-0.010 (n = 716), Cr: 0.019+/-0.015 (n = 618), Mn: 0.24+/-0.16 (n = 730), Fe: 5.5+/-3.9 (n = 728), Co: 0.0046+/-0.0055 (n = 629), Ni: 0.022+/-0.024 (n = 680), Cu: 0.11+/-0.11 (n = 660), Zn: 0.77+/-0.60 (n = 726), As: 0.048+/-0.047 (n = 731), Se: 0.010+/-0.010 (n = 550), Cd: 0.0068+/-0.0082 (n = 709), Sb: 0.033+/-0.036 (n = 687), and Pb: 0.43+/-0.50 (n = 728) (unit, microg m(-3)). All the metal concentrations in TSP in Beijing, China were 1.7-21.8 times higher than those in TSP in the center of Tokyo, Japan. Notably, As concentrations in TSP in Beijing were 20-fold higher than those in Tokyo. Source identification of aerosols in Beijing was carried out by using the chemical mass balance (CMB) receptor model, with the daily concentration of metals in the aerosol. The major primary sources of the aerosol of Beijing were considered to be soil dust and coal combustion. Vehicle exhaust contribution tended to increase.     

对信息化设备用房空调设计的认识及工程实例

分析信息化设备用房空调设计的特点,结合工程实例,总结了信息化设备用房空调设计与一般舒适性空调设计的不同点和应重视的问题；着重探讨了该类空调设计的特殊之处：新风量的确定、空调负荷构成的特点以及不同的气流组织形式与其相应的处理手段,并强调了各专业配合的重要性。     

Irradiation embrittlement monitoring of WWER-440/213 TYPE RPVs

In the paper the main goals and progress of the surveillance specimen programme for the RPVs WWER-440/213 in Jaslovské Bohunice V-2 and Mochovce NPPs are presented. At Jaslovské Bohunice V-2, the standard surveillance specimen programme (SSSP) was finished and so-called ‘Extended Surveillance Specimen Programme’ (ESSP) was prepared on the base of its critical analysis. For first two units of the Mochovce NPP completely new programmes of irradiation embrittlement monitoring called ‘Modern Surveillance Specimen Programme’ is prepared. It is based on the experience with SSSP and ESSP as well as the recommendations of IAEA experts. This programme will serve for Mochovce NPP during all planned service life. The experience of ESSP application on the 3rd and 4th units in Jaslovské Bohunice V-2 NPP are presented in the paper too.     

简易焊接台车在造船工业中的应用

介绍了简易焊接台车在日本造船业焊接平角焊缝作业中的使用情况和这种工艺方法在我国推广应用的益处。     

Development of Arm Equipped Single Wheel Rover: Effective Arm-Posture-Based Steering Method

We have been proposing a new planetary rover system named SMC Rover. This system consists of one main body and detachable units, which can work as child rovers, and also become driving units of the main body. Each detachable unit named Uni-Rover consists of a single manipulator mounted on a wheel. Prototype models of the Uni-Rover and the SMC Rover have been already developed. This paper describes a new method of trajectory modeling for the Uni-Rover using the relation between arm posture and turning radius. At first, an intuitively recognizable model which uses zero slip angle is introduced. However, because this intuitive model has some problems, a new solution which considers the loss of friction on the wheel and on the caster is also discussed. The validity of the presented method is verified by experiments with an actual mechanical model. Moreover, an effective steering method which considers the margin of the energy stability is introduced.Kazuhiro Motomura is a Ph.D candidate at the Tokyo Institute of Technology (Tokyo Tech). In 2001 he was admitted to skip the fourth grade of the bachelor course in the Department of Mechano-Aerospace engineering at Tokyo Tech to enterthe master course in the Department of Mechanical and Aerospace Engineering at the same Institute. He received the B.E. degree in National Institution for Academic Degrees an University Evaluation in 2002, and received the M.E. degree in the Department of Mechanical and Aerospace Engineering at Tokyo Tech in 2003. He has been a research fellow of the Japan Society for the Promotion of Science from 2004. He awarded the Incentive Award for the presentation in the 17th RSJ Annual Conference in 2001, and the Finalists for the Best Video Award in the IEEE International Conference on Robotics and Automation in 2003. His research interests include design of robotic mechanisms and control of mobile robots. He studies about the development of planetary rovers.Atsushi Kawakami awarded the chance to skip fourth gradein the bachelor course of Department of Mechano-Aerospace engineering in Tokyo Institute of Technology, and entered the master course in 1997. He received the M.E. and Dr. E. degrees in Department of Mechanical Engineering Science in Tokyo Institute of Technology in 1999 and 2003, respectively. From 2003, he is a post-doctoral researcher. His research interest is in design of robotic mechanisms. He studies about the development of planetary rover. He awarded the Incentive Award for the presentation in the 17th RSJ Annual Conference in 2001, and the Finalists for the Best Video Award in the IEEE International Conference on Robotics and Automation in 2003. He also acts as an organizer and lecturerin several hands on robots competitions that aim at the promotion and education for the beginners.Shigeo Hirose was born in Tokyo in 1947. He received the B. E. degree with first class honors in Mechanical Engineering from Yokohama National University in 1971, and the M. E. and Dr. E. degrees in Control Engineering from the Tokyo Institute of Technology in 1973 and 1976, respectively. From 1976 to 1979 he was a Research Associate, from 1979 to 1992 an Associate Professor and since 1992 he has been a Professor in the Department of Mechanical and Aerospace Engineering at the Tokyo Institute of Technology. He is the Fellow of both IEEE and Japanese Society of Mechanical Engineers. His research interest is in design of novel robotic mechanisms and its control. He awarded more than 30 prizes from academic societies; these include the first Pioneer in Robotics and Automation Award in 1999 and Best Conference Paper Award in 1995 both from IEEE Robotics & Automation Society. Prof. Hirose has published more than 200 academic papers as well as several books, including Snake Inspired Robots (Kogyo-chosakai Publishing Co. Ltd, 1987, in Japanese), Robotics (Shokabo Publishing Co. Ltd., 1987, in Japanese), and Biologically Inspired Robots (Oxford University Press,1993).     

整装灯泡贯流式小型水电站的研究与应用

榛子岭二级水电站，是利用我国研制的机组建成的第一座整装灯泡贯流式小型水电站。其特点是：整装灯泡贯流式机组的主轴通过一对锥齿轮增速器与立式发电机相连，解决了常规灯泡贯流式机组将发电机置于灯泡体之内所产生的结构设计及安装维护的难题，解决了因超低水头而使发电机尺寸过大和小型水电站机组整装难的问题，通过对榛子岭二级水电站的研究与应用得出结论：整装灯泡贯流式水轮机效率较轴流式水轮机增加３％；电站厂房机组间距     

关于整装勘查区矿业权管理综合改革的思考

矿业权管理综合改革已经进入深水区.要在整装勘查区快速实现找矿突破,实现“三五八”宏伟目标,必须用新政策、新手段、新方法,走局部试验、总结经验、整体铺开的道路.本文提出在整装勘查区率先推进矿业权管理综合改革,通过充分调动投资方、地勘单位、地质找矿科技人员、地方政府、矿区居民五方的积极性,建立五方共赢机制,实现整装勘查区“扩权、搞活、创新、公平”的目标.文章最后提出了改革的路径和建议.     

Use of the finite elements method for modelling the dynamic load impact on hydraulic leg equipped with gas accumulator

Procedures of preparation of numerical analysis, consisting in a simulation of cooperation of three different media: steel, liquid and gas undergoes dynamic load were discussed. Modelling of the initial static load of the mechanical system was presented. By using the MSC. Software products the following exemplary computer simulations were made: dynamic load impact on the hydraulic leg as well as effectiveness of the hydraulic leg protection against overload with help of gas accumulator.