Optimized Biocatalytic Synthesis of 2-Selenopyrimidine Nucleosides by Transglycosylation**

Selenium-modified nucleosides are powerful tools to study the structure and function of nucleic acids and their protein interactions. The widespread application of 2-selenopyrimidine nucleosides is currently limited by low yields in established synthetic routes. Herein, we describe the optimization of the synthesis of 2-Se-uridine and 2-Se-thymidine derivatives by thermostable nucleoside phosphorylases in transglycosylation reactions using natural uridine or thymidine as sugar donors. Reactions were performed at 60 or 80 °C and at pH 9 under hypoxic conditions to improve the solubility and stability of the 2-Se-nucleobases in aqueous media. To optimize the conversion, the reaction equilibria in analytical transglycosylation reactions were studied. The equilibrium constants of phosphorolysis of the 2-Se-pyrimidines were between 5 and 10, and therefore differ by an order of magnitude from the equilibrium constants of any other known case. Hence, the thermodynamic properties of the target nucleosides are inherently unfavorable, and this complicates their synthesis significantly. A tenfold excess of sugar donor was needed to achieve 40−48 % conversion to the target nucleoside. Scale-up of the optimized conditions provided four Se-containing nucleosides in 6–40 % isolated yield, which compares favorably to established chemical routes.     

交错巷道巷间围岩承载结构稳定性分析

针对普遍存在的矿井交错巷道围岩体稳定性及支护问题,运用极限平衡分析和弹塑性力学建立交错巷道巷间围岩稳定性分析判别方程,探讨了交错巷道巷间围岩稳定性主要影响因素,并进行了工业性试验。结果表明:交错巷道巷间围岩体受围岩水平应力集中叠加影响,围岩破坏自巷间围岩体上下表面向中部延伸,加之巷间围岩体尺寸有限,一旦破坏贯通将致使巷间围岩承载存在失稳隐患;交错巷道巷间距越小,越近乎平行布置都会加剧巷间围岩破坏且诱发巷间围岩破坏贯通,合理设计断面尺寸及下方巷道合理支护强度可有效控制并维持围岩稳定;现场采用交错巷道联合支护方案后,交错位置上巷道断面完整无较明显变形且下巷道围岩最大变形量小于100 mm,支护效果显著。     

Vipulanandan model for the rheological properties with ultimate shear stress of oil well cement modified with nanoclay

In this study, the effect of clay nanoparticles (NC) and temperature on the rheological properties with ultimate shear stress and weight loss of the oil well cement (class H) modified with NC was investigated. The NC content was varied between 0 and 1% by the weight of the cement. The total weight loss at 800 °C for the oil well cement decreased from 6.10% to 1.03%, a 83% reduction when the cement was mixed with 1% of NC. The results also showed that 1% of NC increased the rheological properties of the cement slurry. The NC modification increased the yield stress (τ_o) and plastic viscosity (PV) by 5%–65% and 3%–16% respectively based on the NC content and the temperature of the cement slurry. The shear thinning behavior of the cement slurry with and without NC has been quantified using the Vipulanandan rheological model and compared with the Herschel-Bulkley model. The Vipulanandan rheological model has a maximum shear stress limit were as the Herschel-Bulkley model did not have a limit on the maximum shear stress. Based on the Vipulanandan rheological model the maximum shear stress produced by the 0% and 1% of NC at the temperature of 25 °C were 102 Pa and 117 Pa respectively hence an increase of 15% in the ultimate shear stress due to the addition of NC. The addition of 1% of NC increased the compressive strength of the cement by 12% and 43% after 1 day and 28 days of curing respectively. The modulus of elasticity of the cement increased with the additional of 1% NC by 6% and 76% after 1 day and 28 days of curing respectively. Effects of NC content and the temperature on the model parameters have been quantified using a nonlinear model (NLM). The NLM quantified the effect of NC treatment on all the model parameters.     

Static pentane extraction as a potential alternative to Soxhlet extraction for polypropylene olive oil overall migration limit measurements

Static pentane extraction of olive oil from polypropylene was evaluated as a safer, higher throughput alternative to pentane Soxhlet extraction for the recovery of olive oil from polypropylene for overall migration limit determinations. Static, ambient temperature, pentane extraction achieved quantitative recovery of olive oil from polypropylene plaques prepared by using the OM‐5 overall migration limit protocol. In addition to validation data for static extractions, a modified esterification procedure is presented as a means to accommodate the larger sample sets that are enabled with static extraction.     

基于滑模的运载器主动段俯仰通道姿控系统设计

与传统比例-积分-微分（PID）控制方法相比，滑模控制（SMC）方法可以比较容易地将不确定性纳入控制器设计中，从而增强系统的鲁棒性。探索了SMC技术在运载器主动段姿态控制中的工程应用，首先通过分析基于趋近律的SMC系统，提出了降低不连续切换项系数的需求，然后研究了基于干扰上界的SMC方法。三通道小偏差仿真结果验证了两种方法的控制效果，表明第2种控制器的鲁棒性更好，稳态误差小，同时发动机喷管摆角需求较小。     

油田含CO2伴生气变压吸附材料实验研究

变压吸附工艺是国内小规模CO_2驱产出气处置的常用手段,而吸附剂是吸附分离的基础。选用6种沸石类吸附剂,测试了CO_2/CH4单组分吸附平衡等温线,分析了温度对吸附量的影响。优选了两种材料进行了CO_2-CH4混合气体竞争吸附实验,重点分析了温度、压力、气体组分、CO_2含量等因素对吸附剂吸附分离效果的影响,评价了油、水含量对吸附剂的损伤。实验结果表明:吸附剂的分离系数与混合气体中CO_2含量无明显相关性,但吸附体系压力的变化对混合气体分离效果影响较大。在初始气体CO_2摩尔分数50%、温度20℃、压力2.5MPa条件下,优选的吸附剂13X对混合气体的分离系数为25。油水的存在会对吸附剂的分离性能产生较大影响,在温度20℃、压力2.5 MPa、13X吸附剂含水摩尔分数10%及含油摩尔分数10%条件下,吸附总量分别下降32%和78%,分离系数分别下降48%和78%。     

超临界二氧化碳+水交替驱注入井极限关井时间计算

针对超临界CO₂+水交替驱注入井在关井之后形成水合物冻堵段的问题，采用数值模拟和室内实验方法，计算了注入井的极限关井时间。结果表明：在后注水的情况下，地层内CO₂会向注入井端发生反向扩散，CO₂反向扩散到井底的时间为1.6～32.3d，射孔孔眼内的扩散时间为1.0～4.5d；后注气时，极限关井时间主要受渗透率、累计注气量、地层深度影响，极限关井时间为20.0～30.0d；CO₂水合物生成的诱导时间一般低于30min，远低于后注水和后注气时井筒内CO₂和水进入到冻堵位置的时间。该研究结果对CO₂驱的广泛应用具有重要意义。     

铝合金三角形波纹夹芯板对平头弹抗冲击特性数值模拟研究

为研究铝合金三角形波纹夹芯板对平头弹体的抗冲击性能及损伤特性，利用有限元软件ABAQUS/Explicit建立弹体冲击靶板的数值模拟模型，并结合实验验证了模型及其参数的有效性。基于数值计算结果，分析了三角形波纹夹芯板几何结构对其防护性能、失效模式和能量吸收的影响规律及机理，并与等面密度单层板进行对比分析，研究结果表明，靶体几何结构对其抗冲击性能存在影响，三角形波纹夹芯板抗冲击性能低于单层板抗冲击性能。此外，增加芯体拓扑结构夹角能显著提高三角形波纹夹芯板的抗冲击性能，并且靶板几何形状会对其失效模式及耗能特性存在影响。