In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats

The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of the rat lumbar spinal cord and plays a pivotal role in the ejaculatory reflex. We recently reported that SEG neurons express the oxytocin receptor and are activated by oxytocin projections from the paraventricular nucleus of hypothalamus (PVH). However, it is unknown whether the SEG responds to oxytocin in vivo. In this study, we analyzed the characteristics of the brain–spinal cord neural circuit that controls male sexual function using a newly developed in vivo electrophysiological technique. Optogenetic stimulation of the PVH of rats expressing channel rhodopsin under the oxytocin receptor promoter increased the spontaneous firing of most lamina X SEG neurons. This is the first demonstration of the in vivo electrical response from the deeper (lamina X) neurons in the spinal cord. Furthermore, we succeeded in the in vivo whole-cell recordings of lamina X neurons. In vivo whole-cell recordings may reveal the features of lamina X SEG neurons, including differences in neurotransmitters and response to stimulation. Taken together, these results suggest that in vivo electrophysiological stimulation can elucidate the neurophysiological response of a variety of spinal neurons during male sexual behavior.     

In vivo Targeting of DNA Vaccines to Dendritic Cells via the Mannose Receptor Induces Long-Lasting Immunity against Melanoma

Herein, we report effective, C-type lectin mannose receptor (MR)-selective, in vivo dendritic cell (DC)-targeting lipid nanoparticles (LNPs) of a novel lipid-containing mannose-mimicking di-shikimoyl- and guanidine head group and two n-hexadecyl hydrophobic tails (DSG). Subcutaneous administration of LNPs of the DSG/p-CMV-GFP complex showed a significant expression of green fluorescence protein in the CD11c⁺ DCs of the neighboring lymph nodes compared to the control LNPs of the BBG/p-CMV-GFP complex. Mannose receptor-facilitated in vivo DC-targeted vaccination (s.c.) with the electrostatic complex of LNPs of DSG/pCMV-MART1 stimulated long-lasting (270 days post B16F10 tumor challenge) antimelanoma immunity under prophylactic conditions. Remarkably, under therapeutic settings, vaccination (s.c.) with LNPs of the DSG/pCMV-MART1 complex significantly delayed melanoma growth and improved the survival of mice with melanoma. These findings demonstrate that this nonviral delivery system offers a resilient and potential approach to deliver DNA vaccines encoding tumor antigens to DCs in vivo with high efficacy.     

Direct View on the Origin of High Li+ Transfer Impedance in All-Solid-State Battery

Large interfacial resistance plays a dominant role in the performance of all-solid-state lithium-ion batteries. However, the mechanism of interfacial resistance has been under debate. Here, the Li⁺ transport at the interfacial region is investigated to reveal the origin of the high Li⁺ transfer impedance in a LiCoO₂(LCO)/LiPON/Pt all-solid-state battery. Both an unexpected nanocrystalline layer and a structurally disordered transition layer are discovered to be inherent to the LCO/LiPON interface. Under electrochemical conditions, the nanocrystalline layer with insufficient electrochemical stability leads to the introduction of voids during electrochemical cycles, which is the origin of the high Li⁺ transfer impedance at solid electrolyte-electrode interfaces. In addition, at relatively low temperatures, the oxygen vacancies migration in the transition layer results in the formation of Co₃O₄ nanocrystalline layer with nanovoids, which contributes to the high Li⁺ transfer impedance. This work sheds light on the mechanism for the high interfacial resistance and promotes overcoming the interfacial issues in all-solid-state batteries.     

伊拉克A油田Asmari组混积型碳酸盐岩储层隔夹层研究

伊拉克A油田Asmari组为大型混积型碳酸盐岩油藏,发育灰岩、白云岩、砂岩、泥岩和硬石膏等多种岩性,储层复杂、非均质性强,存在多种类型的隔夹层。基于岩心、薄片、测井和分析测试等资料,对研究区隔夹层类型及特征展开综合分析,识别出了泥岩、硬膏岩和泥-微晶碳酸盐岩3类隔夹层。泥岩和硬石膏致密无孔隙、基本不具有渗流能力,对流体流动可以起到阻挡作用;泥-微晶碳酸盐岩属于细粒碳酸盐岩沉积,孔隙度低,但一般裂缝较发育,具有一定渗流能力,不能对流体起到有效的隔挡作用。硬石膏主要在油田中部及北部A段发育,其对油田北部储层纵向连通起到一定隔挡作用,导致纵向压力衰竭不一致。泥岩主要在C段顶部、B4和B1层发育,平面上主要在油田南部较发育。C段顶部发育一套厚度较大、稳定分布的泥岩隔层,将C段和上部B段及A段分隔为两个独立的油水系统,并有效阻挡了C段水层锥进到B段;B4和B1层泥岩较发育,但由于油田南部区域断层和裂缝较发育,导致泥岩隔夹层未能起到有效隔挡作用。搞清研究区隔夹层类型、分布及是否起隔挡作用,对A油田各区域各小层有效动用开发、见水规律研究具有很好的指导意义。     

2种侧围加强内框总成检具结构对比分析

概述了侧围加强内框总成与周边零件的匹配关系,从检具操作的人机工程出发,描述了侧围加强内框总成检具的2种定位方式和结构设计,对比了2种定位方式对于检测结果的影响,总结了其优点和弊端,结果表明侧围加强内框总成检具采用车内方向朝下的定位方式效果更好。     

Hydrodynamics study of a fast liquid–liquid oxidation process with in situ gas production in microreactors

Hydrodynamics characteristics of a fast and highly exothermic liquid–liquid oxidation process with in situ gas production in microreactors were studied using a newly developed experimental method. In the adipic acid synthesis through the K/A oil (the mixture of cyclohexanol and cyclohexanone) oxidation with nitric acid, bubble generation modes were divided into four categories. The gas production became more intensive, unstable, even explosive with increasing the oil phase feed rate and the temperature. A novel automatic image processing method was developed to monitor the instantaneous velocity online by tracking the gas–liquid interface. The axial velocity at the same location was unstable due to the changing gas production rate. Furthermore, the actual residence time was obtained easily with being only 36% of the space–time minimally, beneficial for establishing accurate kinetics and mass transfer models with time participation. Finally, an empirical correlation was developed to predict the actual residence time under different conditions.     

基于现代审美下的赣傩艺术元素分析与设计应用

目的 赣傩艺术元素内容丰富并保存完整,是研究我国古代赣傩地区文化与艺术的珍贵史料.研究赣傩艺术元素的提取及衍生图案的设计方法,在设计应用中表达赣傩傩面具、选取傩舞形和构筑傩庙魂,能给当代赣傩艺术元素的产品设计以指引.方法 通过直接引用、形意结合和局部强化的设计方法,增厚其丰富内涵、设计个性和主题意蕴.通过现代化审美中"绿色化、感性化、地域化"的要求,遵循"以退为进,化繁为简""多元并存,个性体现"的原则,强化赣傩艺术元素的独特风格.结论 用现代的手法,将特色的赣傩艺术元素植入到理性的产品设计中去,使赣傩艺术元素与现代设计思想有机结合.在继承传统文化与现代流行中找到契合点,深入挖掘赣傩艺术元素蕴含的文化精神、文化胸怀和文化自信,使中华传统文化在现代审美下进行创新再现.     

农耕印迹:扎染菱形纹样作为布依语“Ndaeh Sengh”的解读研究

目的 菱形纹样是我国古老的几何纹样之一，其历史悠久、应用广泛，在不同地域和文化背景下被赋予了不同的文化内涵，厘清纹样的在地性符号所指，是提升传统工艺传承与设计创新的方法路径。方法 以贵州花戛乡布依族扎染技艺中的菱形纹样为研究个案，结合人类学的田野调查法和艺术设计学的比较研究法，对花戛布依族服装配饰中的扎染菱形纹样进行综合考证和分析。结论 认为花戛扎染菱形纹样是布依族日用器物“升”的形态转译，是农耕背景下布依人祈求丰收的文化符号载体。有着文化符号功能的菱形“Ndaeh Sengh”纹样，与布依人生活、习俗、地域环境等方面存在多维的联结，“Ndaeh Sengh”越多代表农作物收成越好，寓意丰收和富足。“Ndaeh Sengh”作为农耕记忆背景下布依族集体无意识原型形式的外化，其制作工艺和纹样所承载的文化记忆对于非遗保护、工艺传承、非遗设计等方面都有一定的理论指导意义。     

PBAT‒PLA膜袋受控需氧堆肥条件下的降解研究

目的 通过对广泛使用的PBAT–PLA生物降解膜袋在受控需氧堆肥条件下的降解机制研究，为生物降解塑料的大规模推广提供重要理论基础。方法 根据GB/T 19277.1—2011，在(58±2)℃需氧条件下，对PBAT–PLA膜袋进行为期160 d的生物降解测试(即工业堆肥)，并以常见的可降解材料微晶纤维素作为参比样品。对降解前后的材料进行红外、扫描电镜、能谱分析，并结合其所在堆肥样本的脂肪酶活性，从多角度探寻降解机制。结果 PBAT–PLA膜袋与微晶纤维素所在的堆肥脂肪酶活性都达到空白堆肥的3倍以上。红外显示由微晶纤维素水分子吸附、糖环打开、基团氧化形成的吸收峰加强，PBAT–PLA膜袋中的酯键峰明显减弱;扫描电镜发现降解的PBAT–PLA膜袋表面覆盖了微生物膜;能谱分析发现，碳元素大幅减少，氧元素增加。结论 微生物在PBAT–PLA膜袋表面生长形成生物膜，分泌大量脂肪酶，水解PBAT–PLA的酯键，使聚合物降解为不同链长的中间体或小分子，同时伴随着氧化，随后被作为碳源，在相关微生物体内被代谢利用，形成最终产物。     

Flash cosintering of a lanthanum strontium cobalt ferrite nanofibre/Gd-doped ceria bilayer structure

For solid oxide fuel cells, an important structural requirement is that the electrolyte layer needs to be dense and the electrode layer porous, which is difficult to obtain by conventional cosintering. In this work, flash cosintering of a double layer structure consisting of a Gd-doped ceria substrate with a lanthanum strontium cobalt ferrite nanofibre coating is investigated. Experimental and finite element modelling results reveal that when the LSCF layer is connected to the electrode, the heat is concentrated in the LSCF layer, which leads to a huge temperature gradient and introduces severe cracking. When the LSCF layer is electrically isolated from the electrode, the heat is concentrated in the GDC layer, and the temperature gradient is dramatically reduced. In this situation, the density of GDC can reach 92.86% while a high porosity of 52.26% is maintained in the LSCF layer, which is higher than that of the conventional cosintered sample.