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Cronobacter species are opportunistic foodborne pathogens associated with serious infections in preterm neonates and infants. Based on the epidemiological research, infant formula products are considered to be the main source of infections from this organism. Therefore, accurate methods are required for detection of Cronobacter species. In this study, the specific probe and primers for detection of this organism were designed and verified. The probe-magnetic beads were prepared for sequence capture, followed by PCR assay to detect the target gene. This probe-magnetic separation PCR assay could detect as few as 103 cfu/mL of Cronobacter in artificially contaminated infant formulas in less than 4 h. The combination of magnetic beads and PCR showed the potential for the detection of Cronobacter in infant formulas and may have applications in the dairy industry.  相似文献
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Campylobacter jejuni is a worldwide foodborne pathogen recognized as a leading cause of human gastrointestinal enteritis. A rapid, sensitive, and specific method is required to monitor food and water in cases of contamination by this pathogen. This report presents a novel immunochromatographic test (ICT) using fluorescent microspheres labeled with polyclonal antibodies of C. jejuni as the capture reagent dispensed onto the conjugate pad. Polyclonal antibodies against the outer membrane protein PEB1 of C. jejuni were used as the detective reagent at the test line, whereas the goat anti-rabbit IgG was used on the control line. PEB1 was obtained by gene cloning and expression to prepare its antibody. In this study, a simple and rapid ICT is reported for detecting C. jejuni for the first time with a detection limit of 106 CFU/ mL.  相似文献
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