DNS法测定玉米秸秆中总糖

对DNS法测定玉米秸秆中总糖的各影响因素进行了深入探讨，确定最佳条件：检测波长530nm，显色剂用量1.5mL，沸水浴中保持5min。当水解总糖所用盐酸浓度为6mol／L时，所测总糖主要包括淀粉多糖及寡糖等所水解的还原糖，不包括纤维素等水解的还原糖。  

硼对烤烟生理生化及产质量的影响

本试验对烤烟的Ｂ＾３＋肥效应进行了研究。结果表明，施用适量的Ｂ＾３＋，可促进烤烟生长发育，改善其植物学性状，增加色素含量，提高光合强度和蒸腾速率，使烟叶增产、增质、增收。同时还发现Ｂ＾３＋能使烟株提前２～３ｄ现蕾开花，中下部烟叶提前落黄采收。这一特性，对烤烟生产具有重要的作用。  

几种鸡肌肉中肌苷酸(IMP)的测定

用反相－高效液相色谱法（RP－HPLC）对几种鸡肌肉中的肌苷酸（IMP）含量进行了测定。结果表明：固始鸡几个鸡种肌肉中的肌苷酸含量比A－A鸡（市售西装鸡）来得高，尤其是胸肉：1年生固始鸡母鸡的含量（干重）是A－A鸡的2倍还多，130日龄固始鸡母鸡的含量约为A－A鸡的2倍，90日龄固始鸡公鸡的比A－A鸡高50％；中华宫廷黄鸡肌肉中的肌苷酸含量也比A－A鸡高很多。  

3，5-二硝基水杨酸比色法测定烟草中水溶性总糖

研究了还原糖对３，５－二硝基水杨酸的还原产物的吸收光谱。结果表明，还原产物对波长４７４ｎｍ的光有最大吸收。找出了显色剂的最佳用量。作了标准曲线，同时对３个烟样进行了测定，并与经典方法的测定结果比较，相对误差为２－５％。  

沪酿3．042米曲霉的纯化复壮

本文报告了酱油生产的主要菌种沪酿3．042米曲霉的纯化复 的方法，并对复的新茵株，退化菌株，原始菌株的酶活力，孢子数，生长速度等进行了比较，结果表明，经纯化复壮筛选出的菌株其上述三项指标接近甚至超过原始菌株。  

α-亚麻酸研究进展

α-亚麻酸属于n-3系列多不饱和脂肪酸,分子结构为△9,12,15-18:3,主要来源于陆地植物, 如杜仲、藿香、亚麻、紫苏等。在体内代谢过程中,它主要作为EPA和DHA前体物质,并具有降低胆固醇、血脂,预防心血管疾病、保护视力和抑制过敏反应等功能。该文主要介绍α-亚麻酸的来源、分离提取方法进展及生理功能,并对α-亚麻酸发展前景进行展望。  

服装压力与人体舒适性之关系

 从服装压力与人体舒适性关系、服装压力研究的理论基础与以往的研究基础、学科领域发展中的重大贡献介绍、服装压力的测定等4个方面对目前服装压力与人体舒适性关系的研究进行综述,并讨论了如何用数字化模拟的方法建立三维动态服装压力分布模型。  

Genome-wide identification of fungal GPI proteins

Glycosylphosphatidylinositol-modified (GPI) proteins share structural features that allow their identification using a genomic approach. From the known S. cerevisiae and C. albicans GPI proteins, the following consensus sequence for the GPI attachment site and its downstream region was derived: [NSGDAC]-[GASVIETKDLF]-[GASV]-X(4,19)-[FILMVAGPSTCYWN](10)>, where > indicates the C-terminal end of the protein. This consensus sequence, which recognized known GPI proteins from various fungi, was used to screen the genomes of the yeasts S. cerevisiae, C. albicans, Sz. pombe and the filamentous fungus N. crassa for putative GPI proteins. The subsets of proteins so obtained were further screened for the presence of an N-terminal signal sequence for the secretion and absence of internal transmembrane domains. In this way, we identified 66 putative GPI proteins in S. cerevisiae. Some of these are known GPI proteins that were not identified by earlier genomic analyses, indicating that this selection procedure renders a more complete image of the S. cerevisiae GPI proteome. Using the same approach, 104 putative GPI proteins were identified in the human pathogen C. albicans. Among these were the proteins Gas/Phr, Ecm33, Crh and Plb, all members of GPI protein families that are also present in S. cerevisiae. In addition, several proteins and protein families with no significant homology to S. cerevisiae proteins were identified, including the cell wall-associated Als, Csa1/Rbt5, Hwp1/Rbt1 and Hyr1 protein families. In Sz. pombe, which has a low level of (galacto)mannan in the cell wall compared to C. albicans and S. cerevisiae, only 33 GPI candidates were identified and in N. crassa 97. BLAST searches revealed that about half of the putative GPI proteins that were identified in Sz. pombe and N. crassa are homologous to known or putative GPI proteins from other fungi. We conclude that our algorithm is selective and can also be used for GPI protein identification in other fungi.  

复合固体发酵对棉籽饼脱毒效果的影响研究

通过测定发酵底物中游离棉酚(FG)、粗蛋白质(CP)含量,体外法测定饲料蛋白质消失率等方法研究热带假丝酵母ZD-3(Candida tropicalisZD-3)与黑曲霉ZD-8(Aspergillus nigerZD-8)复合固体发酵对棉籽饼粕脱毒效果的影响;碳酸钠以及矿物质处理对棉籽饼复合发酵脱毒效果的影响。试验结果表明:复合发酵可极显著(P<0.01)地降低棉籽饼底物游离棉酚含量,脱毒率为91.64%;棉籽饼底物粗蛋白含量提高27.83%;粗蛋白体外消化率提高20.90%。结果表明,复合发酵效果明显优于单菌发酵棉籽饼底物的效果,不但达到棉酚脱毒的目的,而且发酵饲料营养价值显著提高。底物中添加碳酸钠及复合矿物质可促进棉籽饼的发酵脱毒效率。  

缺钼烟田施钼对烟草光合作用和氮代谢及烟叶品质的影响

采用盆栽试验研究缺钼烟田施钼对烟草光合作用和氮代谢及烟叶品质的影响.结果表明,施钼能明显提高烟草生育前期的光合速率和NR活性.促进烟草生育前期NO-3 -N的转化,提高烟叶钾含量和改善烟叶油分状况.