高富锗蛹拟青霉菌株的筛选

为了获得高富锗能力的蛹拟青霉菌株,对蛹虫草无性型蛹拟青霉不同菌株进行了富锗培养,并对富锗能力、生物量、有机锗转化率等指标进行了测定.结果表明：富锗能力最强的菌株为28号和14号,在锗浓度为500μg/mL时,生物量分别为15.09 mg/mL和13.84 mg/mL,有机锗转化率分别达到2.3%和1.9%.蛹拟青霉不同菌株具有不同的富锗能力.     

无性型虫草菌株qsun-1的生长特性

利用组织分离法,从青藏高原野外采集的新鲜的冬虫夏草子座中分离得到无性型虫草菌株qsun-1,并研究了该菌株的菌落形态及显微结构.确定无性型虫草菌株qsun-1最佳培养条件为:生长温度15～18℃;培养基的构成为蔗糖2％,蛋白胨2％,MgSO4 0.1％,KH2 PO4 0.2％.     

添加不同碳氮源对蛹虫草子实体生长的影响

以蛹虫草CM2品种为供试菌株,蛹虫草子实体的产量及虫草素含量为指标,以麦粒为基础培养基,添加5种不同碳氮源,观察对蛹虫草菌丝及子实体生长的影响.结果表明,在5种碳氮源中蔗糖和蛋白胨最有利于蛹虫草子实体生长,子实体在100g培养料中产量分别为57g和55.67g.虫草素含量分别高达15.76mg/g和14.32mg/g.     

2012.1.31第1次修回稿

摘要：目的：建立测定蝙蝠蛾被毛孢菌丝体中褪黑素含量的液相色谱-串联质谱方法。 方法：样品中褪黑素以甲醇为提取溶剂，以超声波为提取方式进行提取。采用色谱柱：Thermo Beta Basic-18(150 mm×4.6 mm,5 μm)；流动相：甲醇：0.1%甲酸水（60:40）；流速：0.6 ml/min；柱温：40℃。用串联离子阱质谱在电喷雾电离正离子（ESI+）-二级质谱(MS/MS)模式进行质谱测定。结果：方法的线性范围为10～200 μg/kg，基质加标工作曲线线性方程为y=-8575.85+10204.8x，相关系数r=0.9992。方法的检出限为0.5 μg/kg,定量限为2.0 μg/kg,平均回收率为77.01%～85.02%，相对标准偏差<10%。结论：本方法选择性高、快速，可以应用于蝙蝠蛾被毛孢菌丝体粉末中褪黑素检测，也可为其他复杂体系中褪黑素的测定提供依据。     

北虫草人工栽培条件的优化

通过单因素试验和主要因素的正交试验结果表明:北虫草(Cordyceps m ilitaris)栽培的最佳的原料为大米 10%的蛹粉,原料含水量以60%为佳;最适子实体生长温度为19℃;最适pH为5.5~7.5;黑暗培养不利于子实体生长,在300~700 lx的散射光照时产量最高。     

几种虫草无性型菌株深层发酵菌丝体的有效成分分析

以冬虫夏草生药为对照样品,对蝙蝠蛾拟青霉、蝉拟青霉、古尼拟青霉和细脚拟青霉4种虫草无性型菌株深层发酵菌丝体中的主要有效成分进行了分析,并发现4种样品中细脚拟青霉菌丝体中的甘露醇和氨基酸总含量最高,分别为9.12%和36.44%;古尼拟青霉菌丝体中的多糖含量最高,为10.14%;4种拟青霉菌丝体中均含有丰富的Zn、Fe、Ca等重要的微量元素;古尼拟青霉菌丝体中腺苷的含量最高,达1.97m g/g.分析结果表明此4种拟青霉菌株菌丝体中均含有丰富的活性成分,具有良好的开发利用前景.     

Simultaneous determination of free ergosterol and ergosteryl esters in Cordyceps sinensis by HPLC

The caterpillar-shaped Chinese medicinal mushroom Cordyceps sinensis consists of the fruiting body (CsA) and the host caterpillar (CsB). Ergosterol is a principal sterol in fungi and can indicate the level of mycelia of C. sinensis. Ergosterol is present in two forms, as free ergosterol and esterified ergosterol, which have different physiological functions. The relative abundances of free to esterified ergosterol are different among the various species. In the present study, a gradient reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of free and esterified ergosterol in CsA and CsB of C. sinensis. The results showed that CsA and CsB had similar ergosterol compositions, but the level of ergosteryl esters in CsB was much higher than that in CsA, indicating that CsA and CsB might be in different growth phase or have different physiological functions for the growth and multiplication of C. sinensis.     

Effect of multiple factors on accumulation of nucleosides and bases in Cordyceps militaris

In order to improve the accumulation of valuable metabolites (3′-deoxyadenosine, adenosine, guanosine, cytidine, uridine, adenine and uracil) in Cordyceps militaris, multiple factors such as carbon sources, nitrogen sources, metal ions and fermentation duration were investigated in liquid shake flasks. Using both potato broth and glucose as carbon sources were found to facilitate the production of nucleosides and bases most, and 2% of glucose was the most suitable initial level. Mixture of 0.3% yeast extract with 0.3% peptone was the best selection of nitrogen sources and supplementing with 0.1 mmol/L Mn²⁺ achieved the maximum biomass and biosynthesis of nucleosides and bases among all investigated metal ions. The most suitable harvest time for C. militaris was found to be the 6th day, because the production of 3′-deoxyadenosine, adenosine and uridine began to speed up from the later exponential growth phase until the 6th day. Finally, under optimal culture conditions, the contents of 3′-deoxyadenosine, adenosine, guanosine, cytidine, uridine, adenine and uracil were increased to 0.212 ± 0.014 mg/g, 5.05 ± 0.31 mg/g, 4.03 ± 0.30 mg/g, 0.556 ± 0.029 mg/g, 6.39 ± 0.33 mg/g, 0.208 ± 0.016 mg/g and 0.437 ± 0.027 mg/g, respectively.     

中草药提取液对冬虫夏草深层培养的影响

试验了11种中草药提取液对冬虫夏草液体培养生物量和多糖产量的影响。结果得出：这11种中草药水提液除川牛膝、黄精、山药对冬虫夏草的生物量有抑制作用外，其它几种中草药均有促进作用，增幅范围在0．007-0．94倍。该11种中草药均对冬虫夏草胞外多糖的产量有促进作用，其胞外多糖增幅在0．09～0．88倍；中药醇提液除川牛膝、石斛、黄芪对冬虫夏草的生物量有抑制作用外，其它几种中药促进作用的增幅在0．16～0．67倍。除山药和黄芪外均能提高冬虫夏草胞外多糖的产量，它的增幅在0．03～0．73倍。通过比较，最后实验了当归、党参、枸杞子、川牛膝的最适添加量，前三者在150g／L，后者在50g／L。在此条件下，生物量最大分别可达26．13、22．14、23．55、15．83g／L，胞外多糖的最大产量分别达到7160．2、7580．3、6830．5、6583．6g／L。以上11种中药对冬虫夏草胞内多糖也有一定的促进作用，但增幅不大。