Inactivation efficacy of plasma-activated water: influence of plasma treatment time,exposure time and bacterial species

This study aimed to evaluate the influence of plasma treatment time, bacterial exposure time to PAW and bacterial species on the inactivation efficacy of plasma-activated water (PAW), with additional investigation of the inactivation mechanisms of PAW. Six bacterial species, including Listeria innocua, Staphyloccus aureus, Escherichia coli, Pseudomonas fluorescens, Shewanella putrefaciens and Aeromonas hydrophila were selected as the representative bacteria. The initial bacterial concentration was around 7 log CFU ml⁻¹ after mixing with PAW, and the inactivation efficacy was measured after different exposure times during the 4 °C storage. Scanning electron microscopy (SEM) images of the bacteria after PAW treatment were carried out to inspect the cell structure damage, and physicochemical properties of PAW, including pH, conductivity and long-living reactive species of H₂O₂, , and , were examined. The results showed that the inactivation efficacy of PAW was positively correlated with plasma treatment time and bacterial exposure time, and for the species examined in this study, the Gram-negative species were more sensitive to PAW than the Gram-positive species. Cell structure damage, including shrinkage, distortion, or holes, was observed after PAW treatment. The pH of PAW was acidified to 2.5–2.9, and conductivity was significantly increased to 518.0 μs cm⁻¹. and H₂O₂ were reduced during the 48 h storage, while an increased concentration was observed for . This study demonstrated that the processing parameters of plasma treatment time, exposure time and characteristics of bacteria can significantly affect the inactivation efficacy of PAW.     

Mechanisms of inactivation of hepatitis A virus in water by chlorine dioxide

In this study, to elucidate the mechanisms of inactivation of hepatitis A virus (HAV) by chlorine dioxide, cell culture, enzyme-linked immunosorbent assay (ELISA), and long-overlapping RT-PCR were used to detect the infectivity, antigenicity, and entire genome of HAV before and after disinfection. The results revealed the complete inactivation of infectivity after a 10-min exposure to 7.5mg of chlorine dioxide per liter; and the highest level of sensitivity in the 5'non-translated regions (5'NTR) (the sequence from bp 1 to 671), inactivation of which took as much time as the inactivation of infectivity of HAV by chlorine dioxide; the complete destruction of antigenicity after a 10-min exposure to 7.5mg of chlorine dioxide per liter. It is suggested that the inactivation mechanism of HAV by chlorine dioxide was due to the loss of the 5'NTR and/or destruction of the antigenicity, which is not similar to that of chlorine (Appl Environ Microbiol 68: 4951).     

Effect of corona discharge plasma jet treatment on decontamination and sprouting of rapeseed (Brassica napus L.) seeds

Sprout-related outbreaks of foodborne illnesses are increasingly becoming a food safety concern. Different pathogenic microorganisms that originate from sprout seeds are known to play a crucial role in the pathogenesis of such outbreaks. Therefore, in order to decontaminate and also to enhance the germination of seeds, the applications of non-thermal plasma based techniques are increasingly being investigated in the field of agricultural science as an alternative to conventional pre-germination treatments. This work was aimed to evaluate the effectiveness of corona discharge plasma jet (CDPJ) for microbial decontamination of rapeseed seeds, and also studied the plasma treatment effect on the seed germination and physicochemical properties of sprouts (grown from the plasma-treated seeds). Aerobic bacteria, molds and yeast, Bacillus cereus, Escherichia coli, Salmonella spp. were detected as contaminants in the seeds. All the detected microorganisms were reduced in the range of 1.2–2.2 log CFU/g upon the CDPJ treatment for 3 min. The inactivation patterns are better explained using pseudo-first-order kinetics. The plasma treatment of seeds up to 2 min showed positive effects on their germination rate and seedling growth. Physicochemical and sensory characteristics of rape sprouts were unaffected due to the CDPJ treatment of their respective seeds. Therefore, the CDPJ treatment of rapeseed seeds has not only reduced the seed microbial load, but also contributed to the enhancement of their germination rate and seedling growth, without adversely affecting the physicochemical and sensory characteristics of their corresponding sprouts.     

Inactivation of foodborne pathogens on the surfaces of different packaging materials using low-pressure air plasma

In the present study, low-pressure glow discharge plasma was used for surface decontamination of the common food packaging materials, namely glass, polyethylene, polypropylene, nylon, and paper foil. Low-pressure air plasma was generated over a vacuum pressure range of 0.5–5.0 Torr, and at a power density range of 12.4–54.1 mW/cm³. Compared to plasma-unexposed surfaces, no significant changes in optical properties, color characteristics, surface temperatures, tensile strengths, and deformation strains were observed with plasma-exposed surfaces. On plasma exposure of food pathogens-loaded packaging materials surfaces, as high as 4-log reduction (99.99%) in viable cell counts of tested food pathogens, especially Escherichia coli O157:H7 and Staphylococcus aureus, was observed within 5 min. And, the pathogens inactivation pattern can be better explained by Singh-Heldman model. Therefore, low-pressure air plasma was shown to be effective for inactivation of major foodborne pathogens, and different food packaging materials can be decontaminated using the plasma without adversely affecting their physical properties.     

Inactivation kinetics for Salmonella Enteritidis in potato omelet using microwave heating treatments

The aim of this study was to model the inactivation of Salmonella enterica serovar Enteritidis in pasteurized omelet internally inoculated at different microwave heating treatments (300 W; 450 W, 600 W and 800 W). Results indicated a non significant change in Salmonella populations during the first 30 s treatment at 300 W and 450 W, being log reductions lower than 0.5 log CFU g⁻¹. However, after 40 s treatment, log reductions had risen to 4.8 log CFU g⁻¹ at 800 W. Inactivation rates were higher at 600 W and 800 W (0.67 and 0.63 s⁻¹) than at 300 W and 450 W (<0.34 s⁻¹). The temperature-dependent parameters of a Weibull model obtained by Mattick, Legan, Humphrey & Peleg (2001) were evaluated. It was concluded that combinations characterized by a temperature equal or above 70 °C ensured a minimum 4 log reduction of Salmonella population (i.e. 300 W-80 s; 450 W-60 s or 600 W/800 W-40 s). These results may be of value in food service establishments, as target treatments for microwavable potato omelet portions.     

Modelling of the inactivation kinetics of Escherichia coli,Saccharomyces cerevisiae and pectin methylesterase in orange juice treated with ultrasonic-assisted supercritical carbon dioxide

The combined effect of supercritical carbon dioxide (SC-CO₂) and high power ultrasound (HPU) on the inactivation kinetics of Escherichia coli, Saccharomyces cerevisiae and pectin-methyl esterase (PME) in orange juice was studied in order to select models that can predict their inactivation behaviour based on process parameters. Experiments were performed at different temperatures (31–41 °C, 225 bar) and pressures (100–350 bar, 36 °C). The inactivation rate of E. coli, S. cerevisiae and PME increased with pressure and temperature during SC-CO₂ + HPU treatments. The SC-CO₂ + HPU inactivation kinetics of E. coli, S. cerevisiae and PME were represented by models that included temperature, pressure and treatment time as variables, based on the Biphasic, the Peleg Type B, and the fractional models, respectively. The HPU-assisted SC-CO₂ batch system permits the use of mild process conditions and treatment times that can be even shorter than those of continuous SC-CO₂ systems.     

Strain variability of the behavior of foodborne bacterial pathogens: A review

Differences in phenotypic responses among strains of the same microbial species constitute an important source of variability in microbiological studies, and as such they need to be assessed, characterized and taken into account. This review provides a compilation of available research data on the strain variability of four basic behavioral aspects of foodborne bacterial pathogens including: (i) virulence; (ii) growth; (iii) inactivation; and (iv) biofilm formation. A particular emphasis is placed on the foodborne pathogens Listeria monocytogenes and Salmonella enterica. The implications of strain variability for food safety challenge studies and microbial risk assessment are discussed also. The information provided indicates that the variability among strains of foodborne bacterial pathogens with respect to their behavior can be significant and should not be overlooked. However, in order for the mechanisms underlying the observed strain variability to be elucidated and understood, phenotypic variability data, such as those reviewed here, should be evaluated in conjunction with corresponding findings of studies assessing the molecular/physiological basis of this variability.     

Repression of photoreactivation and dark repair of coliform bacteria by TiO2-modified UV-C disinfection

Photoreactivation and dark repair of damaged DNA can occur after some bacteria are disinfected with UV-C irradiation and thus reduce the disinfection efficiency. We have discovered that the provision of 1 mg/L titanium dioxide (TiO₂) in suspension during UV-C irradiation at normal disinfection doses repressed the photoreactivation and dark repair. The repressive effect was also observed when a TiO₂-coated plate was used during UV-C exposure but removed thereafter, indicating the repressive effect does not require the presence of TiO₂ in the post-UV event. The repressive effect was consistently observed with changes of bacteria species, temperature, salinities, UV sources, doses and intensities, and with/without nutrients. The repressive effect is likely associated with the additional radical attack during UV-TiO₂ irradiation and/or formation of a small amount of stable residual oxidants (primarily hydrogen peroxide). The TiO₂-modified UV-C disinfection represents an innovative means to disinfect water. It is particularly useful in situations where additions of residual chlorine or chloramines are not allowed or not possible. Nevertheless, the TiO₂ shall be properly included, by either using the optimum dose in suspension or immobilizing it onto a surface, since too little TiO₂ cannot provide the repression while too much TiO₂ in suspension gives a detrimental effect on the UV-C disinfection.     

Process and Laboratory Scale UV Inactivation of Viruses and Bacteria Using an Innovative Coiled Tube Reactor

Avoidance and elimination of viral contamination in biotechnological and pharmaceutical processes is a crucial issue. The novel UVivatec coiled tube reactor was designed for UV irradiation of cloudy liquid media. It very effectively inactivates especially critical viruses and avoids high product losses. The method proposed is an ideal complement to existing and established processes.     

猪肉中小肠结肠炎耶尔森菌失活/存活预测模型及控制研究

目的:建立小肠结肠炎耶尔森菌在猪肉中的存活/失活预测模型,并提出其控制措施。方法:用浓度梯度稀释法计细菌总量,按对数法作出不同条件下小肠结肠炎耶尔森菌的生长曲线。采用预测微生物学的基本方法和程序,并用CurveExpert1.38软件作为辅助工具,对试验数据进行拟合。结果:50℃、-18℃及速冻条件下该菌的初始菌数与时间为T时的菌数之比关于时间T的存活/失活预测模型符合Linear关系式,分别为Log(No/Nt)=1.0477287+0.1705726T,Log(No/Nt)=0.091057233+0.036013901T,LogNt=-0.0651058+0.97683871LogNo。结论:实验表明,本菌对高温非常敏感,在冷冻,速冻过程中仍能存活。