Potential of in-field and low temperature drying for reducing chlorophyll contents in canola (Brassica napus L)

The presence of green seeds in canola arises primarily from the common practice of swathing the crop prematurely in order to hasten the harvesting process and reduce the shattering losses. The purpose of this study was to determine the effects of plant maturity, swathing and duration of swathing on moisture and chlorophyll contents of canola seeds (Brassica napus L cv Westar), and to determine the effect of canola conditioning with air at 25·C and 90% RH on chlorophyll content. A standing crop of canola with 50% to 10% (w/w) seed moisture content was sampled at regular intervals in two consecutive harvest years (1989 and 1990). Canola was also swathed at three different stages of maturity, and seeds were checked for chlorophyll at 2-3 day intervals and compared with chlorophyll contents of seed from standing crop. Samples were conditioned in a thin layer with air at 25·C and 90% RH for 24 h, and chlorophyll was determined before and after conditioning. The chlorophyll of seeds decreased exponentially with a decrease in moisture content. Conditioning canola seeds decreased chlorophyll content by an average of 16% (SD = ± 12%).     

Dynamics of photosynthetic pigments in an Andean lake in Colombia

Guatavita Lake is a small, sheltered tropical high mountain lake located in the Colombian Andes, with a closed watershed and a maximum depth of 25 m. It is the freshwater source for human consumption in nearby small towns, as well as being a site of cultural value for the country, as it was a sacred place to indigenous peoples until about five centuries ago. As the structure and function of this aquatic ecosystem is poorly understood, this study provides initial knowledge on its phytoplankton biomass dynamics, which should be useful in designing efficient management plans with environmental baseline information for similar lakes elsewhere. Physical and chemical data, and photosynthetic pigment concentrations, were measured for the period November 1999–November 2000 at the central vertical axis of Guatavita Lake. The vertical profile of the chlorophyll‐a concentration was closely related to the dissolved inorganic nitrogen concentrations and the thermal stratification characteristics. The maximum chlorophyll‐a concentration in the metalimnion was recorded for the thermal stratification period (November 1999–June 2000). Deepening of the maximum chlorophyll‐a concentration began in February, continuing to June, when it reached its lowest value at the 15 m depth. The phytoplankton biomass values showed an increment within the entire water column at the beginning of the mixing period (July). The relation between the metalimnetic peak of chlorophyll‐a and the dissolved inorganic nitrogen concentration suggests the growth of the phytoplankton community is limited mainly by the availability of nitrogen.     

湖泊藻类叶绿素-α和悬浮物浓度的高光谱定量遥感模型研究

本文利用地物光谱仪对太湖水体进行了光谱测量和同步采样分析，对获得的数据尝试用光谱分离法进行分析，从中分离出蓝藻和悬浮物的特征波峰，建立波峰高度与同步水质采样得到的叶绿素-α浓度和悬浮物浓度的对应关系，得出其遥感定量反演算法，并实际应用在OMIS成像光谱仪图像上．     

Rapid determination of chlorophylls in vegetable oils by laser-based fluorometry

The presence of chlorophylls in vegetable oils is considered undesirable due to their adverse effects on oxidative deterioration, bleachability and hydrogenation. The usual methods for determination of chlorophylls in vegetable oils are absorption spectrophotometry, fluorometry and liquid chromatography using fluorescence detectors. This paper outlines a fluorometric method using a laser as the excitation source. The chlorophylls in crude palm oil, rapeseed oil, soya bean oil and olive oil were determined directly by laser-based fluorometry using (a) a helium-neon laser (632-8 nm, 5 mW) as the excitation source and an optical-multichannel analyser (OMA) with an intensified diode array as the detector, and (b) a helium-neon laser and a photomultiplier tube as the detector. The method is especially suitable for the detection of chlorophylls in crude palm oil since no pre-extraction step is required. The levels of chlorophylls measured in vegetable oils using the laser technique was also compared against values obtained by conventional spectrophotometric methods. The simplicity and rapid quantification of laser based fluorometry suggest that this technique can be readily applied to the detection or a wide variety of fluorescing species in vegetable oils.     

Modified Atmosphere Packaging Affects Ascorbic Acid, Enzyme Activity and Market Quality of Broccoli

Reduced ascorbic acid (RAA) content, total chlorophyll and green color retention, enzyme activities and texture changes were followed in broccoli spears packaged in polymeric film and nonpackaged during 96 hr storage (10°C). Concentrations within packages monitored by gas chromatography were (CO₂,) 8% and (0²) 10%. RAA retention, moisture content, total chlorophyll and color retention were greater in packaged broccoli. No differences were found between packaged and nonpackaged broccoli for ascorbate oxidase and peroxidase activities, or texture.     

Seasonal variation in light penetration and subsurface chlorophyll-α in southern Lake Michigan observed by a glider

The Cooperative Institute for Great Lakes Research (CIGLR) in collaboration with the Great Lakes Observing System and National Oceanic and Atmospheric Administration, Great Lakes Environmental Research Laboratory (NOAA GLERL) deployed an autonomous underwater glider in southern Lake Michigan several times per year between 2012 and 2019 to collect offshore (>30 m depth) limnological measurements, including temperature, photosynthetically active radiation (beginning during 2015), and chlorophyll-α fluorescence. From these data, we calculated mixed layer depth, several measures of light penetration (diffuse attenuation coefficient, first optical depth, euphotic zone depth), and depth of the subsurface chlorophyll-α maxima. During summer, mean offshore mixed layer depth was typically 10–15 m, K_d for PAR was 0.1–0.17 m^?1, first optical depth was 6–9 m, euphotic zone depth was 35–40 m, and depth of subsurface chlorophyll-α maxima was 30–35 m. We also observed substantial spatial and temporal variation in these values across the basin and within and among seasons. Glider-based observations provide a wider horizontal and vertical perspective than other methods (e.g., ship- and satellite-based observations, buoys, and fixed moorings), and are therefore a valuable, complementary tool for Great Lakes limnology. The set of observations reported here provide seasonal and basin-scale information that may help to identify anomalies useful for future glider-assisted investigation into the role of biophysical processes in Great Lakes limnology and ecology.     

Effects of three phenolic acids on chlorophyll content and growth of soybean and grain sorghum seedlings

Experiments were designed to test the hypothesis that interference with chlorophyll metabolism may be one mechanism of inhibition of plant growth in allelopathic interactions. Effects of ferulic,p-coumaric, and vanillic acids on soybean and grain sorghum growth and chlorophyll content were quantified and compared after seedlings were treated with these compounds in a nutrient culture. Following a 6-day treatment cycle, dry weights of soybean seedlings were reduced by both 10^–3 M and 5 × 10^–4 M treatments of ferulic,p-coumaric and vanillic acids. Soybean weight reductions in each case were paralleled by a significant reduction in the concentration (g Chl/mg dry wt) of chlorophylls a and b and total chlorophyll in the unifoliate leaves. Sorghum seedling growth was also reduced by each of the compounds at the 5 × 10^–4 M level, but leaf chlorophyll concentration was not below that of control plants.     

三峡库区支流中水体的垂向掺混对于藻类生长的影响

三峡水库蓄水之后库区支流水华问题给当地生态环境造成了较大影响,也引起了广泛的关注和重视,然而针对这一问题尚未取得很好的解释和解决方案。本文采用EFDC模型对于2008年坝前库区干支流的三维水动力和水质进行了数值模拟,并从水体垂向掺混的角度分析支流的水华现象。模拟结果成功再现了香溪河库湾中的温度分层和异重流现象,流场、温度场的计算值和实测值吻合较好。统计结果表明:长江干支流水动力特性的差异显著,干支流垂向紊动黏性系数、垂向紊动扩散系数沿垂线分布呈现出不同的形态,且长江干流的垂向掺混强度明显高于香溪河。同时,叶绿素浓度与垂向紊动扩散系数呈显著的负相关关系,随着水体垂向紊动扩散系数的增加,叶绿素浓度逐渐降低,这一规律的发现可为采用物理方法防治三峡库区支流水华打下基础。     

天津近岸海域沉积物叶绿素状况分析

于2006—2007年间,分4个季节对天津近岸10,m等深线以内水域进行了沉积物中叶绿素a和脱镁叶绿酸a的调查研究工作,旨在了解该海域的沉积物叶绿素分布情况,为环境评价、生物资源研究提供背景资料.结果表明,沉积物中叶绿素a和脱镁叶绿酸a分布存在站位和季节差异,脱镁叶绿酸a含量一般要高于叶绿素a含量.与已有研究结果比较,本研究海域的叶绿素a含量处于中等水平,而脱镁叶绿酸a含量偏高.     

LED激发叶绿素荧光探测仪的设计研究

对蓝光激发叶绿素的荧光光谱进行了分析,讨论了叶绿素荧光光谱的来源,采用调制式470nm绿光LED激发叶绿素,制成叶绿素荧光光谱仪,线性范围0—100μg／L,检出限0．1μg／L,该方法快速、灵敏、有很好的特异性．