首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   4610篇
  免费   628篇
  国内免费   220篇
电工技术   31篇
综合类   300篇
化学工业   1332篇
金属工艺   63篇
机械仪表   115篇
建筑科学   120篇
矿业工程   13篇
能源动力   26篇
轻工业   1941篇
水利工程   8篇
石油天然气   37篇
武器工业   7篇
无线电   231篇
一般工业技术   449篇
冶金工业   85篇
原子能技术   55篇
自动化技术   645篇
  2024年   7篇
  2023年   102篇
  2022年   138篇
  2021年   368篇
  2020年   191篇
  2019年   173篇
  2018年   155篇
  2017年   161篇
  2016年   171篇
  2015年   281篇
  2014年   311篇
  2013年   352篇
  2012年   361篇
  2011年   389篇
  2010年   265篇
  2009年   281篇
  2008年   274篇
  2007年   270篇
  2006年   226篇
  2005年   211篇
  2004年   158篇
  2003年   158篇
  2002年   92篇
  2001年   75篇
  2000年   49篇
  1999年   30篇
  1998年   18篇
  1997年   13篇
  1996年   21篇
  1995年   34篇
  1994年   27篇
  1993年   17篇
  1992年   17篇
  1991年   19篇
  1990年   11篇
  1989年   9篇
  1988年   4篇
  1987年   9篇
  1986年   3篇
  1985年   2篇
  1984年   1篇
  1980年   3篇
  1960年   1篇
排序方式: 共有5458条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
5.
The anoctamin (TMEM16) family of transmembrane protein consists of ten members in vertebrates, which act as Ca2+-dependent ion channels and/or Ca2+-dependent scramblases. ANO4 which is primarily expressed in the CNS and certain endocrine glands, has been associated with various neuronal disorders. Therefore, we focused our study on prioritizing missense mutations that are assumed to alter the structure and stability of ANO4 protein. We employed a wide array of evolution and structure based in silico prediction methods to identify potentially deleterious missense mutations in the ANO4 gene. Identified pathogenic mutations were then mapped to the modeled human ANO4 structure and the effects of missense mutations were studied on the atomic level using molecular dynamics simulations. Our data show that the G80A and A500T mutations significantly alter the stability of the mutant proteins, thus providing new perspective on the role of missense mutations in ANO4 gene. Results obtained in this study may help to identify disease associated mutations which affect ANO4 protein structure and function and might facilitate future functional characterization of ANO4.  相似文献   
6.
7.
Diabetic neuropathy (DN), the most common chronic and progressive complication of diabetes mellitus (DM), strongly affects patients’ quality of life. DN could be present as peripheral, autonomous or, clinically also relevant, uremic neuropathy. The etiopathogenesis of DN is multifactorial, and genetic components play a role both in its occurrence and clinical course. A number of gene polymorphisms in candidate genes have been assessed as susceptibility factors for DN, and most of them are linked to mechanisms such as reactive oxygen species production, neurovascular impairments and modified protein glycosylation, as well as immunomodulation and inflammation. Different epigenomic mechanisms such as DNA methylation, histone modifications and non-coding RNA action have been studied in DN, which also underline the importance of “metabolic memory” in DN appearance and progression. In this review, we summarize most of the relevant data in the field of genetics and epigenomics of DN, hoping they will become significant for diagnosis, therapy and prevention of DN.  相似文献   
8.
9.
Chronic stress is a combination of nonspecific adaptive reactions of the body to the influence of various adverse stress factors which disrupt its homeostasis, and it is also a corresponding state of the organism’s nervous system (or the body in general). We hypothesized that chronic stress may be one of the causes occurence of several molecular and cellular types of stress. We analyzed literary sources and considered most of these types of stress in our review article. We examined genes and mutations of nuclear and mitochondrial genomes and also molecular variants which lead to various types of stress. The end result of chronic stress can be metabolic disturbance in humans and animals, leading to accumulation of reactive oxygen species (ROS), oxidative stress, energy deficiency in cells (due to a decrease in ATP synthesis) and mitochondrial dysfunction. These changes can last for the lifetime and lead to severe pathologies, including neurodegenerative diseases and atherosclerosis. The analysis of literature allowed us to conclude that under the influence of chronic stress, metabolism in the human body can be disrupted, mutations of the mitochondrial and nuclear genome and dysfunction of cells and their compartments can occur. As a result of these processes, oxidative, genotoxic, and cellular stress can occur. Therefore, chronic stress can be one of the causes forthe occurrence and development of neurodegenerative diseases and atherosclerosis. In particular, chronic stress can play a large role in the occurrence and development of oxidative, genotoxic, and cellular types of stress.  相似文献   
10.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号