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1.
Mytimycins are cysteine-rich antimicrobial peptides that show antifungal properties. These peptides are part of the immune network that constitutes the defense system of the Mediterranean mussel (Mytilus galloprovincialis). The immune system of mussels has been increasingly studied in the last decade due to its great efficiency, since these molluscs, particularly resistant to adverse conditions and pathogens, are present all over the world, being considered as an invasive species. The recent sequencing of the mussel genome has greatly simplified the genetic study of some of its immune genes. In the present work, we describe a total of 106 different mytimycin variants in 16 individual mussel genomes. The 13 highly supported mytimycin clusters (A–M) identified with phylogenetic inference were found to be subject to the presence/absence variation, a widespread phenomenon in mussels. We also identified a block of conserved residues evolving under purifying selection, which may indicate the “functional core” of the mature peptide, and a conserved set of 10 invariable plus 6 accessory cysteines which constitute a plastic disulfide array. Finally, we extended the taxonomic range of distribution of mytimycins among Mytilida, identifying novel sequences in M. coruscus, M. californianus, P. viridis, L. fortunei, M. philippinarum, M. modiolus, and P. purpuratus.  相似文献   
2.
钟何  赵羽习  孟涛 《建筑材料学报》2022,25(11):1160-1167
为了提高贝壳废弃物的消纳量,实现资源循环利用,以贻贝粗骨料替代天然粗骨料,制备了贻贝粗骨料取代率为15%、30%、45%、60%、100%的贻贝粗骨料混凝土,并研究了其宏微观性能.结果表明:贻贝粗骨料中具有较高含量的无机钙,但作为天然粗骨料的替代材料,由于吸水率为2.4%、表观密度为2 402 kg/m3、压碎指标为68.2%,均超出规范要求,贻贝粗骨料物理性能弱于天然粗骨料;随着贻贝粗骨料取代率的提高,贻贝粗骨料混凝土的力学性能降低;贻贝粗骨料与砂浆界面过渡区的缺陷区域是混凝土性能劣化的主要原因;配合比设计得出,当贻贝粗骨料取代率为30%时,可以制备强度等级为C30的贻贝粗骨料混凝土.  相似文献   
3.
Dreissenid mussel veligers compose a substantial component of pelagic biomass in the Great Lakes, yet their dynamics are poorly understood. To evaluate seasonal, spatial, and inter-annual variation in veliger density, we used a 64-μm mesh plankton net (2008, 2013–2016) and a 153-μm mesh plankton net (2007–2016) to collect dreissenid veligers at nearshore (15–25?m depth), transitional (45?m) and offshore (93–110?m) sites in southeast Lake Michigan during March–December. We also evaluated trends in density of recently settled mussels relative to veliger abundance and the density of the standing stock of adult mussels. Veliger density peaked during both summer and fall at all sites, but peak densities in summer were generally higher nearshore, whereas peak densities in the fall were generally higher offshore. The density of veligers in the 153-μm net was overall 28% of that in the 64-μm net, but there was high variability in this comparison among months. Smaller veligers were much more abundant in the 64-μm net, but there was little difference in the size distribution and abundance between nets for the 210–300?μm size classes. Thus, the 153-μm net could still be a useful tool for assessing density trends of larger veligers just prior to their settlement. Newly settled mussels (≤2?mm) were most abundant in summer or fall at the nearshore and offshore sites but were nearly absent at the transitional site despite the high density of veligers there. Factors other than veliger density must play an important role in mussel recruitment.  相似文献   
4.
仿贻贝类含儿茶酚改性聚乙烯醇黏附凝胶的合成   总被引:1,自引:0,他引:1  
将儿茶酚(catechol)接枝到聚乙烯醇(PVA)侧基上,得到改性聚乙烯醇(m-PVA),以模拟贻贝类分泌的黏附蛋白液。通过(UV-spectra)、(FT-IR)及(H1NMR)证实了m-PVA的大分子结构,并计算出接枝率大约为10%。此外,通过热失重分析(TG)表征了mPVA的热稳定性能。对于模拟贻贝类黏附凝胶的形成机理,通过UV-spectra研究了m-PVA在不同p H值条件下对三价铁离子(Fe3+)的络合作用及所形成的黏附凝胶,以及在高碘酸钠(Na IO4)的氧化下,通过化学交联得到的具有黏附性质的凝胶。通过本文的初步研究,最终合成出具有一定黏附性质的m-PVA凝胶,在一定程度上对贻贝类黏附凝胶进行了仿生。  相似文献   
5.
Wearable and implantable bioelectronics are receiving a great deal of attention because they offer huge promise in personalized healthcare. Currently available bioelectronics generally rely on external aids to form an attachment to the human body, which leads to unstable performance in practical applications. Self‐adhesive bioelectronics are highly desirable for ameliorating these concerns by offering reliable and conformal contact with tissue, and stability and fidelity in the signal detection. However, achieving adequate and long‐term self‐adhesion to soft and wet biological tissues has been a daunting challenge. Recently, mussel‐inspired hydrogels have emerged as promising candidates for the design of self‐adhesive bioelectronics. In addition to self‐adhesiveness, the mussel‐inspired chemistry offers a unique pathway for integrating multiple functional properties to all‐in‐one bioelectronic devices, which have great implications for healthcare applications. In this report, the recent progress in the area of mussel‐inspired self‐adhesive bioelectronics is highlighted by specifically discussing: 1) adhesion mechanism of mussels, 2) mussel‐inspired hydrogels with long‐term and repeatable adhesion, 3) the recent advance in development of hydrogel bioelectronics by reconciling self‐adhesiveness and additional properties including conductivity, toughness, transparency, self‐healing, antibacterial properties, and tolerance to extreme environment, and 4) the challenges and prospects for the future design of the mussel‐inspired self‐adhesive bioelectronics.  相似文献   
6.
While nutrient loading has affected all levels of Lake Winnipeg’s ecology, its greatest influence has likely been on the microbial community. In addition to eutrophication, zebra mussels (Dreissena polymorpha) have recently invaded the ecosystem and threaten food web dynamics. Their filter-feeding predation and association with bacteria, specifically phototrophs, was investigated. A sampling trip to Lake Winnipeg in October 2017, focused on the isolation, enumeration, and identification of aerobic anoxygenic phototrophs in littoral water, sediment, and tissues of mussels. Gimli, Patricia, and Grand beaches, separated by >15 km across the South Basin, had similar bacterial counts when cultivated on rich organic, BG-11, purple non-sulphur, and K2TeO3-supplemented media. Culture-based enumeration on rich organic medium revealed 1.74% of heterotrophs from littoral waters were aerobic anoxygenic phototrophs, and represented 13.98% within sediments. In contrast, 0.48, 1.15, and 0.16% of cultured heterotrophs were aerobic anoxygenic phototrophs within zebra mussel gill, gut, and gonadal tissues, respectively. High-throughput sequencing of bacterial 16S V4 rDNA maintained similar trends in respective bivalve organs, where 0.22, 1.13, and 0.20% of total 16S genes belonged to these phototrophs. Several Sphingomonadaceae isolates were recovered from gut tissues, all with filamentous morphology large enough for predation. Bioaccumulation of metals was also studied in D. polymorpha. All tested associated aerobic anoxygenic phototrophs were capable of resisting the metalloid oxide tellurite. The consistent distribution of aerobic anoxygenic phototrophs within microbial communities across Lake Winnipeg, and their predominance in the gut tissues of zebra mussels suggested bacterial consumption by this invasive species.  相似文献   
7.
Beginning in 2013, sites at the 128-m bottom depth contour were added to the sampling design of the annual Lake Michigan bottom trawl survey for prey fish, which has been conducted by the U.S. Geological Survey Great Lakes Science Center (GLSC) each fall since 1973, to better assess fish depth distributions in a changing ecosystem. The standard sampling design included bottom depths from 9 to 110 m, although the GLSC also sporadically conducted bottom trawl tows at the 128-m bottom depth contour during 1973–1988. Enactment of this new sampling design in 2013 revealed that mean biomass density of deepwater sculpins (Myoxocephalus thompsonii) at the 128-m depth exceeded the sum of mean biomass densities at shallower depths, indicating that the bulk of the deepwater sculpin population is residing in waters deeper than 110 m. Thus, our findings supported the hypothesis that the depth distribution of the deepwater sculpin population had shifted to deeper waters beginning in 2007, thereby explaining, at least in part, the marked decline in deepwater sculpin abundance since 2006 based on the standard sampling design. In contrast, our results did not support the hypothesis that the slimy sculpin (Cottus cognatus) population had shifted to deeper waters sometime after 2000. A portion of the burbot (Lota lota) population may have also shifted in depth distribution to waters deeper than 110 m after 2007, based on our results. Our findings have served as an impetus to further expand the range of depths sampled in our bottom trawl survey.  相似文献   
8.
Mercury (Hg) is an environmental pollutant that impacts human and ecosystem health. In our previous works, we reported alterations in the properties of Mytilus galloprovincialis protamine-like (PL) proteins after 24 h of exposure to subtoxic doses of toxic metals such as copper and cadmium. The present work aims to assess the effects of 24 h of exposure to 1, 10, and 100 pM HgCl2 on spermatozoa and PL proteins of Mytilus galloprovincialis. Inductively coupled plasma–mass spectrometry indicated accumulation of this metal in the gonads of exposed mussels. Further, RT-qPCR analyses showed altered expression levels of spermatozoa mt10 and hsp70 genes. In Mytilus galloprovincialis, PL proteins represent the major basic component of sperm chromatin. These proteins, following exposure of mussels to HgCl2, appeared, by SDS-PAGE, partly as aggregates and showed a decreased DNA-binding capacity that rendered them unable to prevent DNA damage, in the presence of CuCl2 and H2O2. These results demonstrate that even these doses of HgCl2 exposure could affect the properties of PL proteins and result in adverse effects on the reproductive system of this organism. These analyses could be useful in developing rapid and efficient chromatin-based genotoxicity assays for pollution biomonitoring programs.  相似文献   
9.
The colonization progress of the invasive bivalve dreissenids, the formerly dominant Dreissena polymorpha and the recently (2008) introduced Dreissena rostriformis bugensis was studied between 2009 and 2013 in the largest Central European shallow lake, Lake Balaton, Hungary. The density of dreissenid planktonic veligers, new settlers (post-veligers and early juveniles), and the population structure (density, length frequency, relative abundance) of the two species were monitored on experimentally introduced natural stone substrata, on different time scales. Dreissenids started dynamic settling following a sudden veliger bloom. As substratum saturation progressed, competition between species for places was suggested, which, after two years, led to an increased number of large individuals (> 20 mm) and also recruits of D. r. bugensis. By contrast, the population of D. polymorpha was confined to middle size (11–18 mm) individuals of the first settler generation. On local substrata, where the benthic community was already established, the replacement of D. polymorpha by D. r. bugensis took longer, but it happened in a similar way. The invasion speed of D. r. bugensis in Lake Balaton resembled the speed obtained in other European water bodies where D. r. bugensis, similar to Lake Balaton, was introduced much later than D. polymorpha. However, a longer replacement process was found in North America, where both species invaded new habitats at the same time. This suggests that the speed, and probably the success, of D. r. bugensis invasion depends on new surface availability, and whether the two dreissenid species are introduced together or at different times.  相似文献   
10.
实验研究了嵊泗三个海域的贻贝中大肠杆菌、菌落总数、铅和镉的含量情况,并对贻贝的生长环境的水质进行了检测。结果表明,抽检的贻贝大肠杆菌、菌落总数符合农业部一类贝类生产养殖区标准。其铅、镉含量也符合《食品中污染物限量》(GB 2762-2012)要求。同时,水质样品铅和镉的含量符合《中华人民共和国渔业水质标准(GB 11607-1989)》的限量要求。  相似文献   
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