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食品中农药残留在人体中的生物累积效应和农药对环境和人类健康所造成的危害逐渐受到人们的重视, 发展高灵敏度、高选择性、简单快速、成本低廉的残留农药检测分析方法势在必行。生物传感器由于具有灵敏度高、简便、快速、成本低廉等优点而成为一种理想的农药检测手段。本文综述了近年来农药残留生物传感器的设计原理及其在食品和环境安全中的应用方面的研究进展, 总结了不同农药残留生物传感器的优缺点及其所面临的挑战和前景展望。  相似文献
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Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella enterica are the most common foodborne bacterial pathogens and are responsible for many outbreaks. Therefore, multiplex detection of these three using a single assay platform is highly desirable. The objective was to develop and optimize a fiber optic sensor for simultaneous detection of these three from food. The streptavidin coated optical waveguides were immobilized with biotinylated polyclonal antibodies and exposed to the bacterial suspensions or enriched food samples for 2 h. Pathogens were detected after reacting with Alexa-Fluor 647-labeled monoclonal antibodies. Ready-to-eat beef, chicken and turkey meats were inoculated with each pathogen (∼100 cfu/25 g), enriched in SEL (Salmonella, E. coli, Listeria), a multipathogen selective enrichment broth for 18 h and tested with the biosensor. The biosensor was able to detect each pathogen, individually or in a mixture with very little cross-reactivity. The limit of detection for the sensor was ∼103 cfu/ml for all three pathogens. Furthermore, the biosensor successfully detected each pathogen, grown in a mixture from enriched meat samples under 24 h. The pathogen presence was further verified by PCR and immunofluorescence assay. The multiplex fiber optic sensor shows promise for detection of the three pathogens if present in the same sample eliminating the use of multiple single pathogen detection platforms.  相似文献
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