种分Δαk与种分首槽αk的关系

针对烧结法和拜尔法种分槽的 Δαk 变化进行了概述 ,阐述了种分 Δαk 与种分首槽 αk 的关系。     

Trypsin inhibitor activity of soybean as affected by genotype and fertilisation

The presence of protease inhibitors in soybean prohibits the utilisation of the raw beans for food and feed. However, little information is available about environmental influences and the effects of nitrogen and sulphur supply on the antinutritional constituents of soybean. As these factors may influence protease inhibitors, soybean genotypes segregated according to the presence or absence of the Kunitz trypsin inhibitor have been evaluated for trypsin inhibitor activity (TIA) in field trials. TIA was affected significantly by environment (geographical location), fertilisation treatment and genotype. Environmental means of TIA were between 69.5 and 104.8 mg g^?1. Nitrogen application, which caused an increase in seed protein content, resulted in a reduction in TIA by about 15% as compared with the control. Remarkably, simultaneous application of nitrogen and sulphur in the form of ammonium sulphate had a similar reductive effect on TIA to that of nitrogen application alone, although soybean protease inhibitors are rich in sulphur amino acids. Significant genetic variation in TIA was found both within the genotype class with the Kunitz inhibitor present as well as within the class lacking this inhibitor. The results suggest that TIA of soybean may be modified considerably by genetic improvement and appropriate agronomic management. Copyright © 2003 Society of Chemical Industry     

防止减速器“跑,冒,漏”油的几种新方法

介绍了防止减速器“跑，冒，漏”油的几种新方法。     

Allelopathic effects of giant foxtail on germination and radicle elongation of loblolly pine seed

Water extracts of giant foxtail grass inhibited germination and radicle elongation of loblolly pine seed in a laboratory test. Most of the toxic effects came from extracts of dried foxtail tops, with lesser amounts from fresh tops and roots. Soil texture affected the phytotoxicity of the extracts. Eight chemical compounds known to be phytotoxins inSetaria were identified in extracts of foxtail leaves but could not be found in soil samples under the leaves.     

Process optimisation in sunflower oil extraction by supercritical CO2

A continuous process for the extraction of sunflower oil using supercritical CO₂, featuring multiple extractors, one oil separator and three cascaded CO₂ recovery vessels operating at different pressures, was devised and studied. For every single equipment of the plant making up the process a mathematical model was built. Experimental tests—consisting in measurements of oil solubility in supercritical CO₂—were carried out in a laboratory-scale apparatus to characterise the behaviour of sunflower oil in the separation from the supercritical fluid. The mathematical model of the whole process was coded in the commercial gPROMS process modelling environment where both its simulation and optimisation—this latter assuming the overall oil production cost as the objective function—were carried out. The process- and economics-related results are discussed and compared with those obtained with traditional and cold-pressing extraction.     

新生牛肝活性肽的制备及其毒性检测

目的制备新生牛肝活性肽并评价其安全性。方法采用膜法分离制备新生牛肝活性肽。将3批制品于37～40℃,75%相对湿度条件下存放3个月,以多肽含量为指标观察其稳定性,并进行急性毒性试验、小鼠骨髓细胞微核试验、Ames试验、小鼠精子畸形试验和大鼠喂养试验。结果制备的3批新生牛肝活性肽存放3个月后,多肽含量无明显下降,质量稳定。小鼠和大鼠经口灌人大于20.0g/kg体重的新生牛肝活性肽,均无急性毒性。3种致突变试验均未显示出致突变性,大鼠喂养试验各项指标均未见明显毒性。结论新生牛肝活性肽未表现出明显毒性。     

Further characterisation of protease inhibitors from pigeon pea (cajanus cajan (l) millsp) seeds

Cajanus trypsin inhibitor (CTI) and Cajanus trypsin-chymotrypsin inhibitor (CTCI) previously purified from cv TAT-10 were further characterised. The modification of the inhibitors revealed the presence of lysine at the trypsin reactive site in both CTI and CTCI. Modification of tyrosine at the reactive site of CTCI did not abolish chymotrypsin inhibition suggesting the presence of leucine or phenylalanine as reported in other chymotrypsin inhibitors. CTCI did not contain tryptophan. Dissociation constants for the inhibitors with bovine trypsin were in the region of 0.69 nmol (CTCI) and 0.029 nmol (CTI). Although the protease inhibitors lost their inhibitory activity on exposure to 2-mercaptoethanol they remained attached to the enzyme. The inhibitors were not very effective against the protease from Helicoverpa armigera which is a serious field pest of Cajanus. Dissociation constants for the inhibitors with the larval enzyme were in the region of 100 nmol.     

Purification and Characterization of an Endoprotease from Melon Fruit

An endoprotease was purified from melon fruit (Cucumis melo L.) by ammonium sulfate precipitation, gel filtration and ion-exchange chromatography using t-butyloxycarbonyl-Ala-Ala-Pro-Leu p-nitroanilide as a substrate. The molecular weight was estimated as 26,000 and isoelectric point pH 9.5. It preferentially hydrolyzed peptide bonds of the carboxyl terminal sides of Leu, Ala, His, Gin, and Am. Activity was strongly inhibited by diisopropyl phosphofluoridate, indicating the serine protease nature of the enzyme. The migration distance on electrophoresis, molecular weight and substrate specificity differed from cucumisin, a known protease from melon. This unusual protease may have potential for special food treatment applications.     

谷氨酰胺(Gln)活性肽营养液的研究进展

对G1n小肽在氨基酸营养液中的使用以及G1n小肽的稳定性和代谢机制进行了论述。G1n是一种“条件必需氨基酸”，但Cln的水溶液不稳定，因而在氨基酸输液或氨基酸营养被中，用G1n小肽代替G1n单体使用。G1n小肽含有取代的α—NH2，使Cln小肽的稳定性大大增加。