Influence of pressurised cryogenic nitrogen technology on Arthrospira platensis (spirulina) preservation during storage

In this study, preservation of spirulina using the new pressurised cryogenic nitrogen technology (PCN) was compared to classical methods used in laboratories and industry. Spirulina morphology was better preserved by PCN compared to unpressurised cryogeny and classical freezing at −20 °C that led to cells fragmentation. A 25% loss of Phycocyanin-C content against 60% were measured after 98 days storage for 6-Bar PCN process and frozen samples, respectively. The Total AntiOxidant Power (PAOT Liquid Technology^®) was used for determination of total antioxidant and oxidant power of spirulina extracts. PAOT value of PCN samples was 50% higher than the frozen sample. From ABTS measurements on PCN spirulina fractions sonicated or not, it was suggested that pressurisation at 6 bars allowed a better preservation of free antioxidants (outside the cells) due to replacement of oxygen by nitrogen in the frozen beads. After dehydration, phycocyanin-C content variation during storage at 20 °C and 33% RH showed higher loss for freeze-dried spirulina treated at 0 compared to 6 Bars.     

Assessment of antioxidant activity and phycocyanin release of Spirulina loaded poly(ε-caprolactone) electrospun nanofibers

PCL with biodegradable property and Spirulina with various biological activities offer good alternative ingredients for the fabrication of functional nanofibers in tissue engineering. The aim of the study is to obtain PCL/Spirulina nanofibers with low diameters and to determine their wettability, antioxidant activity, and phycocyanin release to evaluate their potential as bioactive scaffolds in different applications. PCL/acetic acid/pyridine solutions with three different Spirulina concentrations were electrospun and the obtained nanofibers were investigated. The thinnest PCL/Spirulina nanofibers (117.20 nm) were obtained at 1.5% Spirulina concentration. Although PCL nanofibers with 6% Spirulina concentration showed the highest antioxidant activity and amount of phycocyanin released, PCL nanofibers with 3% Spirulina concentration having similar in vitro results showed superiority when considering the diameter and uniformity of the nanofibers and the cost of the material. The PCL/Spirulina nanofibers with small diameters and antioxidant activity could be regarded as potential extracellular matrix material for tissue engineering.     

钝顶螺旋藻藻蓝蛋白的稳定性试验研究

用冻融的方法制备钝顶螺旋藻(Spirulina platensis)藻蓝蛋白并对纯化后的藻蓝蛋白稳定性进行了初步研究。提取后的粗提液经羟基磷灰石柱层析纯化后其纯度(A621／A280)达3.1温度、酸碱度、乙醇对藻蓝蛋白稳定性有较大影响；NaCl、苯甲酸钠、柠檬酸等食品添加剂对其稳定性影响较小。     

基于哨兵3A-OLCI影像的内陆湖泊藻蓝蛋白浓度反演算法研究

蓝藻是内陆富营养水体水华发生的主要优势藻种, 而藻蓝蛋白 (Phycocyanin, PC) 是蓝藻的标志性色素, 因此利用遥感估算水体中藻蓝蛋白浓度从而对蓝藻水华预警具有重要意义。利用太湖、滇池、洪泽湖的实测数据, 构建藻蓝蛋白随机森林遥感估算模型, 并将模型应用到哨兵3A-OLCI影像。通过对随机森林的输入自变量进行重要性分析, 发现第7波段 (620 nm) 、第8波段 (665 nm) 和第9波段 (675 nm) 三个波段对藻蓝蛋白反演的影响程度最大。同时, 反演结果表明, 随机森林反演的藻蓝蛋白浓度平均绝对百分比误差 (MAPE) 为34. 86%, 均方根误差 (RMSE) 为38. 67μg/L, 与Simis等半分析算法和齐琳的PCI (Phycocyanin Index) 指数模型相比, 平均绝对百分比误差 (MAPE) 分别提高了85. 06%和15. 65%, 均方根误差分别提高了26. 08μg/L和19. 86μg/L。利用地面实测数据对同步卫星影像大气校正进行精度评价, 发现MUMM (The Management Uint Mathematical Model) 算法可以用于OLCI影像的大气校正, 尤其在560779 nm处共8个波段的MAPE低于30%, 光谱曲线与实测光谱曲线形状保持一致。结果表明所构建的基于哨兵3A-OLCI影像的藻蓝蛋白随机森林反演模型, 可以成功的应用于我国的内陆富营养化湖泊, 为我国内陆湖泊藻蓝蛋白浓度的遥感反演提供一个新的算法。     

细胞破壁对螺旋藻藻蓝蛋白提取效果的影响

螺旋藻细胞破壁是藻蓝蛋白提取的关键工序。采用溶胀法、超细剪切法、超声波法、反复冻融法、溶胀—超细剪切法和溶胀—超细剪切—超声波法对螺旋藻细胞进行破壁处理;通过对比分析破壁后提取液中的藻蓝蛋白得率来评价破壁方法的优劣。结果表明,溶胀法、超细剪切法、超声波法、反复冻融法、溶胀—超细剪切法、溶胀—超细剪切—超声波法得到的最高藻蓝蛋白得率分别为8.90%,7.38%,8.00%,8.26%,9.22%,8.88%。综合考虑,溶胀—超细剪切法相对其它5种破壁方法而言,其操作简单便捷,破壁效果更加显著,藻蓝蛋白得率高;溶胀—超细剪切法最佳的提取工艺为:溶胀时间12h,剪切时间5min。     

Functionalization of Nanostructured Hematite Thin‐Film Electrodes with the Light‐Harvesting Membrane Protein C‐Phycocyanin Yields an Enhanced Photocurrent

The integration of light‐harvesting proteins and other photosynthetic molecular machinery with semiconductor surfaces plays an important role in improving their performance as solar‐cell materials. Phycocyanin is one such protein that can be employed for this purpose. Phycocyanins have light‐harvesting properties and belong to the phycobilisome protein family. They are present in cyanobacteria, which capture light energy and funnel it to reaction centers during photosynthesis. Here, a way of increasing the photocurrent of hematite by covalent cross‐coupling with phycocyanin is reported. For this, a hematite–phycocyanin integrated system is assembled by consecutive adsorption and cross‐coupling of protein molecules, separated by an agarose layer and a linker molecule, on the top of a mesoporous hematite film. The hematite–phycocyanin assembly shows a two‐fold increased photocurrent in comparison with pristine hematite film. The increase in the photocurrent is attributed to the enhanced light absorption of the hematite film after integration with the protein, as is evident from the UV–vis spectra and from the photocurrent‐action spectrum. The assembly shows long‐term stability and thus constitutes a promising hybrid photoanode for photo‐electrochemical applications.     

两步盐析联合双水相萃取提取纯化蓝藻中藻蓝蛋白

以巢湖新鲜蓝藻为处理对象,采取冻融破壁的方式获取藻蓝蛋白的粗提液,采取两步盐析联合双水相萃取的方法提取纯化藻蓝蛋白。运用0.618法选点实验研究了两步盐析中(NH4)2SO4饱和度对提取纯化影响,构建了聚乙二醇（polyethylene glycol,PEG）-(NH4)2SO4的双水相体系,综合考虑PEG相对分子质量、PEG质量分数、(NH4)2SO4质量分数、pH值和藻蓝蛋白质量浓度对提取纯化藻蓝蛋白的影响。结果表明：室温25 ℃条件下,一步盐析时(NH4)2SO4的最佳饱和度为21%,二步盐析时(NH4)2SO4的最佳饱和度为30%。两步盐析后得到的藻蓝蛋白构建成PEG-(NH4)2SO4的双水相体系后,当PEG相对分子质量1 000、PEG 1 000质量分数10%、(NH4)2SO4质量分数25%时,藻蓝蛋白的纯度可达3.45。     

螺旋藻中藻蓝蛋白的生理功能及其提取纯化研究进展

藻蓝蛋白是螺旋藻的主要组成成分,它的生理功能已经得到多方面的证实.对藻蓝蛋白的结构、抗氧化、抗炎等生理活性及其提取纯化工艺作了较为系统的综述,以期为藻蓝蛋白的进一步研究与开发利用积累完善的资料.