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1.
Biotechnological hydrogen production is considered as an environmentally sustainable alternative to petrochemical sources or electrolysis. Here, disruption of lactate dehydrogenase (LDH) and metabolic regulation via the small RNA RyhB were adopted to improve hydrogen production in the novel Klebsiella sp. strain FSoil 024. The hydrogen production of FSoil 024-L (Δldh) and FSoil 024-L/R (Δldh/RyhB) from glucose in a 5-L fermenter respectively increased by 40 and 50% compared to the wild type. When glycerol was adopted as a more favorable substrate, FSoil 024-L generated 3.3 L/L of hydrogen after 52 h of fermentation, implying its great potential for the utilization of crude glycerol to produce hydrogen. Overexpression of RyhB downregulated formate biosynthesis in FSoil 024, thereby redirecting NADH toward the hydrogen production pathway. This finding provides new insights into the role of cellular reducing power in hydrogen metabolism and establishes a rationale for improving hydrogen production.  相似文献   
2.
The ability of Clostridium butyricum RAK25832 to use casamino acids as a nitrogen source was investigated. Strain RAK25832 showed the capacity to utilize different types of carbon sources. With glucose as a carbon source (10 g/L), the preferred final concentration of casamino acids was 26.67 g/L, with a cumulative hydrogen production, production rate, and yield of 2505 mL H2/L, 160 mL/h, and 1.81 mol H2/mol glucose, respectively. Eighteen metal elements were screened to identify the most important metals for biohydrogen production, and four elements were optimized. The optimal medium composition was MgCl2·6H2O (0.1 g/L), K2HPO4·3H2O (6.67 g/L), NaHCO3 (2.6 g/L), and FeCl2·4H2O (0.002 g/L). Vitamin supplementation of the medium showed no significant effect on hydrogen production. Under the optimized conditions, cumulative hydrogen production reached 3074 mL H2/L. This is the first study to demonstrate the use of casamino acids as a nitrogen source by C. butyricum.  相似文献   
3.
针对传统双环PID控制三相逆变器系统存在输出电压质量和鲁棒性不够理想,输出电压谐波含有率较高等问题,设计基于神经网络参数自整定的分数阶PIλDμ双环控制器:PIλDμ电流内环PIλDμ电压外环控制、PIλDμ电流内环PID电压外环控制和PID电流内环PIλDμ电压外环控制,并应用于不同的线性及非线性负载,考察其控制性能。仿真研究表明,分数阶PIλDμ电流内环整数阶PID电压外环控制性能最好,其次是整数阶PID电流内环分数阶PIλDμ电压外环控制和分数阶PIλDμ电流内环分数阶PIλDμ电压外环控制,性能最差的是传统的双环PID控制。  相似文献   
4.
In the real decision making, q-rung orthopair fuzzy sets (q-ROFSs) as a novel effective tool can depict and handle uncertain information in a broader perspective. Considering the interrelationships among the criteria, this paper extends Choquet integral to the q-rung orthopair fuzzy environment and further investigates its application in multicriteria two-sided matching decision making. To determine the fuzzy measures used in Choquet integral, we first define a pair of q-rung orthopair fuzzy entropy and cross-entropy. Then, by utilizing λ-fuzzy measure theory, we propose an entropy-based method to calculate the fuzzy measures upon criteria. Furthermore, we discuss q-rung orthopair fuzzy Choquet integral operator and its properties. Thus, with the aid of q-rung orthopair fuzzy Choquet integral, we consider the preference heterogeneity of the matching subjects and further explore the corresponding generalized model and approach for the two-sided matching. Finally, a simulated example of loan market matching is given to illustrate the validity and applicability of our proposed approach.  相似文献   
5.
蒲凌杰    曾繁慧    汪培庄   《智能系统学报》2020,15(3):528-536
目前,基于因素空间理论的背景基提取算法计算过程复杂,初始化必须依赖各因素极值,基点数量提取冗余等原因,未能在应用中取得很好效果。为此,结合内点判别法和知识可继承、可扩展的思想,提出一种计算简单、初始化独立、基点数量小的改进的背景基提取算法。然后,利用改进的背景基提取算法构造出一种全新的数据分类算法-基点分类算法,基点分类算法以提取每一类样本的背景基为预测模型,再通过新定义的λ-背景基,优化预测模型。数值实验表明:基点分类算法原理简单、构造难度小、分类模型泛化能力强,预测能力准确率高,同时严格的模型限定区域又能为识别新类别提供新方法。  相似文献   
6.
λ-Graph systems are labelled Bratteli diagrams with shift operations. They present subshifts. Their matrix presentations are called symbolic matrix systems. We define skew products of λ-graph systems and study extensions of subshifts by finite groups. We prove that two canonical symbolic matrix systems are G-strong shift equivalent if and only if their presented subshifts are G-conjugate.  相似文献   
7.
Recovery of Li+ from geothermal water has been investigated using a combination of adsorptive separation with magnetite and λ-MnO2 adsorbents. The inhibition of Li+ adsorption together with decomposition of λ-MnO2 was observed when geothermal water containing As was fed to the column in which λ-MnO2 was packed. Magnetite (Fe3O4) was therefore used for removal of As from the geothermal water, prior to adsorption of Li+. Using As removal from the geothermal water with magnetite, decomposition of λ-MnO2 was significantly prevented during adsorption of Li+, although a small amount of As still remained.  相似文献   
8.
当前利用CT技术从细观层面研究混凝土的力学特性大都处于定性的观察和统计分析研究阶段,未能利用恰当的数学理论对宝贵的CT试验结果进行定量化分析。本文将基于集合论的破损演化理论引入混凝土CT试验数据研究中,解决利用CT数定量研究混凝土破坏过程的问题;在破损演化理论的基础上利用(λ1~λ2)截理的定义将CT图像分为孔洞裂纹区(P0~0.35)、硬化水泥石区(P0.35~0.7)及骨料区(P0.7~1),实现了对混凝土各组分的定量化分区研究,进而定量地研究了CT试验过程中全域和感兴区域上P0~0.35、P0.35~0.7及P0.7~1随加载的变化过程。研究结果表明利用本方法可较好地描述混凝土的受力破坏过程,能定量地体现试样破坏过程中应变的局部化。  相似文献   
9.
为了克服压电叠堆的迟滞特性,实现压电叠堆的精确控制,建立了压电叠堆控制系统,研究了该系统所用到的神经网络、分数阶微积分等算法。首先,搭建了采集压电叠堆位移数据的硬件系统,并对含有噪声的位移数据进行了滤波处理;利用径向基函数(RBF)神经网络对压电叠堆建模,得到了模型参数。然后,利用RBF神经网络建模得到的Jacobain信息来整定分数阶PI~μD~λ控制器中的参数对压电叠堆进行控制。最后,与RBF整数阶PID对压电叠堆的控制效果进行了对比。结果显示:RBF建模误差仅为位移实测数据的0.22%,RBF神经网络分数阶PIμDλ控制系统输出稳定,很好地跟随了给定。得到的结果表明RBF神经网络分数阶PI~μD~λ控制器控制性能良好,在压电叠堆的控制中比RBF整数阶PID控制器表现得更加稳定、精确。  相似文献   
10.
Based on the active coupled line concept, a novel approach for calculating the noise performance of microwave/mm-wave field-effect transistors (FETs) is proposed. The voltage and current Green's functions are introduced to determine the correlation matrix of the FET. Some results using this approach have been presented for a microwave MESFET.  相似文献   
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