The effects of ozone,ultrasound and coating with shellac and lysozyme–chitosan on fresh egg during storage at ambient temperature – part 1: interior quality changes

The objective of this research was to investigate and compare the effect of different treatments including gaseous ozone (6-ppm/4-min), ultrasound (450 W/4 min) and coating of shellac, lysozyme–chitosan (L-C) on fresh eggs internal quality during storage for six weeks at ambient temperature. The internal quality attributes such as weight loss (WL), albumen viscosity, Haugh unit (HU), yolk index, total soluble solids, albumen and yolk's pH were monitored. Control groups had the worst HU (50.04), WL (8.35%), albumen pH (9.27) and viscosity (7.72 mPa·s) values after storage. The best interior qualities were obtained by shellac coating [HU (72.37), WL (1.37) and viscosity (30.10 mPa·s)] and sonication (albumen pH: 8.22). Thus, the shellac-coated eggs maintained all the internal quality parameters at the highest values, while the ozone and ultrasound only helped to keep the internal quality and enhance the shelf life of eggs.     

Salmonella in table eggs from farm to retail – When is cooling required?

Storage time and temperature are known to be important factors in determining Salmonella growth in table eggs. It is assumed that growth of Salmonella within eggs starts when the egg yolk membrane breaks down, allowing Salmonella to enter and grow. The length of time for which the yolk membrane remains intact is time and temperature dependent.The present study addresses the question whether the current legal requirement in Germany to cool table eggs marketed 18 days after laying needs to be updated. Detailed data on the current storage and transport conditions in Germany were collected. On the basis of these data, typical combinations of temperature and time were selected. For these different time-temperature scenarios, the consequences regarding the probability of growth of Salmonella Enteritidis inside the eggs were calculated. A probabilistic model consisting of nine modules reflecting storage/transport stages from farm to retail in Germany was used to calculate whether the yolk membrane remains intact during the individual steps. Growth of Salmonella was simulated without cooling (room temperature) as well as with two different cooling scenarios (4–6 °C and 8–12 °C), which are temperature ranges used in Germany by various retailers. Simulations of these scenarios resulted in relative low numbers of eggs with Salmonella growth until purchase (89 out of 50,000 eggs in the uncooled scenario, 10 out of 50,000 eggs stored at 8–12 °C and 5 out of 50,000 eggs stored at 4–6 °C). These results show that for an average egg trading time of 7.5 days, as was observed for Germany, the probability for the consumer to purchase eggs where no Salmonella growth has started and yolk membrane integrity still exists is high. However, the model supports the necessity for egg cooling after the yolk membrane integrity time is exhausted. Furthermore, the model shows clearly that cooling will extend the time period before the egg yolk membrane breaks down. According to our results, the requirement to cool eggs from the 18th day onwards after laying, as required by legislation in Germany, is reasonable, as it matches the time point of the yolk membrane breakdown for the average egg kept at 18–20 °C. Therefore, continuation of this regulation is strongly supported.     

Survival of tapeworm eggs, free and in proglottids, during simulated sewage treatment processes

The survival of T. saginata eggs in stored sludge, anaerobic and aerobic mesophilic and thermophilic digesters is examined, particularly with reference to the protection afforded to eggs bound within proglottids. Gross survival times paralleled the results of other workers. However, proglottid bound eggs always survived for longer periods than did freshly dissected eggs. The consequences of this for experimentation is discussed. T. saginata eggs were killed in all treatments with anaerobic digestion being more effective than aerobic digestion and lagooning. In all processes the major controlling factor was temperature; at 35°C eggs were killed faster than at 20°C, eggs at 55°C survived for only a few hours whether free or in proglottids.     

HS-SPME结合GC-MS分析煎鸡蛋的挥发性风味成分

采用顶空固相微萃取与GC-MS联用方法对煎鸡蛋中挥发性成分进行提取与分析,考察萃取头、萃取温度和吸附时间对分析结果的影响,得到优化的顶空固相微萃取条件为:黑色萃取头(75μm Carboxen/PDMS),吸附温度75℃,吸附时间70 min。在优化的条件下分析,共鉴定出50种挥发性风味成分,其中,醛类16种(40.588%)、含氮化合物14种(23.639%)、醇类8种(7.156%)、烃类3种(4.800%)、酚类及杂环化合物3种(1.755%)、酮类3种(0.868%)及含硫化合物3种(0.563%)。鉴定出含量较高(相对质量分数大于2.5%)的物质有:2,5-二甲基吡嗪、3-甲基丁醛、2-甲基吡嗪、壬醛、苯甲醛、反,反-2,4-癸二烯醛、辛醛、2-甲基丁醛、反-2-癸烯醛、3,7-二甲基-1,6-辛二烯、1-辛烯-3-醇。     

Determination of pyrethroids in chicken egg samples: development and validation of a confirmatory analytical method by gas chromatography/mass spectrometry

The aim of this study was to develop and validate an efficient analytical method based on gas chromatography/tandem mass spectrometry (GC/MS/MS) for detection and quantification of six pyrethroids residues (Phenothrin, Permethrin, Cyfluthrin, Cypermethrin, Deltamethrin and Fenvalerate) in chicken eggs. The method was based on a preliminary liquid–liquid extraction of albumen‐free yolk samples, followed by a clean‐up by solid‐phase extraction. GC/MS/MS analyses were carried out in the selected reaction monitoring mode. Validation parameters such as specificity, detection capability, decision limit, precision, recovery, stability and ruggedness were determined, resulting in compliance with Decision 2002/657/EC. No complicated apparatus are required; moreover, low volumes of organic solvents and a nonintensive manual labour are required. These low costs and simple procedure, based on rapid and safe operations, may represent a useful tool in the routine analysis of pyrethroids pesticides, in the place of the currently used conventional techniques.     

Pilot survey of hen eggs consumed in the metropolitan area of Rio de Janeiro,Brazil, for polyether ionophores,macrolides and lincosamides residues

A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method, which has recently been developed and validated, was used for the identification and quantification of polyether ionophore, macrolide and lincosamide residues in commercial eggs sold in the metropolitan area of Rio de Janeiro, Brazil. The method was applied to 100 samples and the results showed a high incidence of polyether ionophore residues (25%). Salinomycin was detected in 21% of samples, but only two non-compliant results (5.3 and 53 µg?kg^?1) were found if maximum limits (tolerances) established by European Union were adopted in Brazil and if a method decision limit (CCα) of 3.4?µg?kg^?1 was considered. In 8% of analyzed samples, more than one studied coccidiostat was found. The lincosamide, lincomycin, and the macrolide, tylosin, were detected at trace levels in 4 and 1% of the samples, respectively. Lasalocid, clarithromycin and erythromycin were not found.     

Rapid sample preparation procedure for As speciation in food samples by LC-ICP-MS

This paper describes a rapid method for arsenic (As) speciation by LC-ICP-MS in several types of food samples. Prior to analysis, samples were milled and the As species extracted from biological tissues by sonication in only 2?min with a solution containing MeOH (10%, v/v) plus HNO₃ (2%, v/v). As species were separated by LC using an anion exchange column. Method detection limits for AsB, As³⁺, DMA, MMA and As⁵⁺ were 1.3, 0.9, 0.6, 0.7 and 0.8?ng?g^?1, respectively. Method accuracy and precision were traceable to Certified Reference Materials SRM1577 bovine liver from the National Institute of Standards and Technology, CE278 mussel tissue from the Institute of Reference Materials and Measurements and DOLT-3 dogfish liver tissue and DORM-3 fish protein from the National Research Council of Canada. Finally, the method was applied to speciate As in food samples (egg, fish muscle, beef and chicken) purchased in Brazilian markets.