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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Peter K. Windsor Stephen P. Plassmeyer Dominic S. Mattock Jonathan C. Bradfield Erika Y. Choi Bill R. Miller III Byung Hee Han 《International journal of molecular sciences》2021,22(6)
Deposition of amyloid β (Aβ) fibrils in the brain is a key pathologic hallmark of Alzheimer’s disease. A class of polyphenolic biflavonoids is known to have anti-amyloidogenic effects by inhibiting aggregation of Aβ and promoting disaggregation of Aβ fibrils. In the present study, we further sought to investigate the structural basis of the Aβ disaggregating activity of biflavonoids and their interactions at the atomic level. A thioflavin T (ThT) fluorescence assay revealed that amentoflavone-type biflavonoids promote disaggregation of Aβ fibrils with varying potency due to specific structural differences. The computational analysis herein provides the first atomistic details for the mechanism of Aβ disaggregation by biflavonoids. Molecular docking analysis showed that biflavonoids preferentially bind to the aromatic-rich, partially ordered N-termini of Aβ fibril via the π–π interactions. Moreover, docking scores correlate well with the ThT EC50 values. Molecular dynamic simulations revealed that biflavonoids decrease the content of β-sheet in Aβ fibril in a structure-dependent manner. Hydrogen bond analysis further supported that the substitution of hydroxyl groups capable of hydrogen bond formation at two positions on the biflavonoid scaffold leads to significantly disaggregation of Aβ fibrils. Taken together, our data indicate that biflavonoids promote disaggregation of Aβ fibrils due to their ability to disrupt the fibril structure, suggesting biflavonoids as a lead class of compounds to develop a therapeutic agent for Alzheimer’s disease. 相似文献
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为研究攀爬机器人在港口机械金属结构表面的自主移动,提出了一种应用于攀爬机器人的视觉导航定位方法。针对港口机械金属结构复杂,表面存在油污和阴影,解决现有的视觉边缘识别方法对金属结构边缘识别效率低且不连续的问题。首先通过自适应阈值的Canny进行边缘特征提取;之后根据边缘线角度特征建立约束关系,利用Hough变换剔除干扰线,并用最小二乘法拟合最终金属结构边缘;求取结构中心线方程和运动方程并计算偏航角和偏距,实现对机器人的运动控制。以港口机械某一支腿为例进行试验,实验数据表明,该方法可以很好地引导机器人的运动,满足实际应用。 相似文献
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针对泵站普遍因存在供需不平衡、管理差等而造成大量能源浪费的问题,对泵站系统扬程、效率偏差情况进行分析,明确了系统供需间的差异情况,利用灰色关联度法,对泵站目标电耗的影响因素进行关联度分析,确定了扬程、效率、流量3个影响因素关联度大小。建立以单位产量电耗最小为目标函数的优化调度模型,并结合泵站实际情况,运用人工蜂群算法(Artificial Bee Colony,ABC)进行寻优计算,得出运行优化方案,得到系统在不同流量、扬程需求下的最佳开机台数及调速比,表明了ABC算法在泵站优化的实用价值。 相似文献
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针对频域法构造小波滤波器方法较少、稳定性无法保证、逼近精度不高等问题,基于天牛须搜索算法提出了一种小波滤波器直接频域逼近方法。首先根据小波滤波器电路的基本要求设计逼近有理式,然后加入电路实现所需的稳定性、正实性和初值为零的约束条件,建立小波滤波器逼近的频域优化模型。再以高斯小波和Marr小波为例,使用天牛须搜索算法求解频域逼近的数学模型得到滤波器有理式参数。在时域和频域上分别将该方法和其他频域方法进行对比,仿真和计算结果表明在相同条件下,频域逼近法具有更优的效果,五阶高斯小波和七阶Marr小波的逼近均方误差仅为3.446 8×10~(-4)和7.346 2×10~(-4)。 相似文献
7.
Eugene Metakovsky Laura Pascual Patrizia Vaccino Viktor Melnik Marta Rodriguez-Quijano Yulia Popovych Sabina Chebotar William John Rogers 《International journal of molecular sciences》2021,22(4)
The Gli-B1-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1-γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B. It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i, k, m, o), II (Gli-B1n, q, s), III (Gli-B1b), IV (Gli-B1e, f, g), V (Gli-B1h), VI (Gli-B1d) and VII (Gli-B1a). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat. 相似文献
8.
Md Saifur Rahman Md Badrul Alam Young Kyun Kim Mst Hur Madina Ismail Fliss Sang Han Lee Jin Cheol Yoo 《International journal of molecular sciences》2021,22(10)
In this study, we investigate the immunomodulatory effects of a novel antimicrobial peptide, YD1, isolated from Kimchi, in both in vitro and in vivo models. We establish that YD1 exerts its anti-inflammatory effects via up-regulation of the Nrf2 pathway, resulting in the production of HO-1, which suppresses activation of the NF-κB pathway, including the subsequent proinflammatory cytokines IL-1β, IL-6, and TNF-α. We also found that YD1 robustly suppresses nitric oxide (NO) and prostaglandin E2 (PGE2) production by down-regulating the expression of the upstream genes, iNOS and COX-2, acting as a strong antioxidant. Collectively, YD1 exhibits vigorous anti-inflammatory and antioxidant activity, presenting it as an interesting potential therapeutic agent. 相似文献
9.
Kyunghee Kim Jisue Kim Hyoungseob Kim Gun Yong Sung 《International journal of molecular sciences》2021,22(4)
Owing to the prohibition of cosmetic animal testing, various attempts have recently been made using skin-on-a-chip (SOC) technology as a replacement for animal testing. Previously, we reported the development of a pumpless SOC capable of drug testing with a simple drive using the principle that the medium flows along the channel by gravity when the chip is tilted using a microfluidic channel. In this study, using pumpless SOC, instead of drug testing at the single-cell level, we evaluated the efficacy of α-lipoic acid (ALA), which is known as an anti-aging substance in skin equivalents, for skin tissue and epidermal structure formation. The expression of proteins and changes in genotyping were compared and evaluated. Hematoxylin and eosin staining for histological analysis showed a difference in the activity of fibroblasts in the dermis layer with respect to the presence or absence of ALA. We observed that the epidermis layer became increasingly prominent as the culture period was extended by treatment with 10 μM ALA. The expression of epidermal structural proteins of filaggrin, involucrin, keratin 10, and collagen IV increased because of the effect of ALA. Changes in the epidermis layer were noticeable after the ALA treatment. As a result of aging, damage to the skin-barrier function and structural integrity is reduced, indicating that ALA has an anti-aging effect. We performed a gene analysis of filaggrin, involucrin, keratin 10, integrin, and collagen I genes in ALA-treated human skin equivalents, which indicated an increase in filaggrin gene expression after ALA treatment. These results indicate that pumpless SOC can be used as an in vitro skin model similar to human skin, protein and gene expression can be analyzed, and it can be used for functional drug tests of cosmetic materials in the future. This technology is expected to contribute to the development of skin disease models. 相似文献
10.
针对目前铝电解行业对于槽似在电阻的采集不够准确并且延时较高的问题,本文提出一种基于卡尔曼滤波的区间式槽似在电阻采集算法。该算法以卡尔曼滤波为基础,用预测值与采样值的均方差表征它们的高斯白噪声功率,使其能够在电阻平稳的状态下有着较强的跟踪性能;再结合一阶惯性滤波的强滤波特性和卡尔曼滤波的强跟踪优势,设置适用的滤波区间,确保组合算法在槽似在电阻波动较大的情况下能够滤除掉噪声的影响,并在电解槽稳定后能对槽似在电阻进行快速收敛跟踪。结果表明:与一阶惯性滤波相比,改进后的卡尔曼滤波在电阻平稳的状态下其均方根误差减少50%,在电解槽反生针振和摆动情况之后的收敛时间减少90%。 相似文献