The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8

Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. WE derived from monounsaturated long-chain acyl moieties are of industrial interest due to their very good lubrication properties. Whereas WE were obtained in the past from spermaceti organs of the sperm whale, industrial WE are nowadays mostly produced chemically from fossil fuels. In order to produce WE more sustainably, attempts to produce industrial WE in transgenic plants are steadily increasing. To achieve this, different combinations of WE producing enzymes are expressed in developing Arabidopsis thaliana or Camelina sativa seeds. Here we report the identification and characterization of a fifth wax synthase from the organism Marinobacter aquaeolei VT8, MaWSD5. It belongs to the class of bifunctional wax synthase/acyl-CoA:diacylglycerol O-acyltransferases (WSD). The protein was purified to homogeneity. In vivo and in vitro substrate analyses revealed that MaWSD5 is able to synthesize WE but no triacylglycerols. The protein produces WE from saturated and monounsaturated mid- and long-chain substrates. Arabidopsis thaliana seeds expressing a fatty acid reductase from Marinobacter aquaeolei VT8 and MaWSD5 produce WE. Main WE synthesized are 20:1/18:1 and 20:1/20:1. This makes MaWSD5 a suitable candidate for industrial WE production in planta.     

Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe

Storage lipids, triacylglycerols (TAG), and steryl esters (SE), are predominant constituents of lipid droplets (LD) in fungi. In several yeast species, metabolism of TAG and SE is linked to various cellular processes, including cell division, sporulation, apoptosis, response to stress, and lipotoxicity. In addition, TAG are an important source for the generation of value-added lipids for industrial and biomedical applications. The fission yeast Schizosaccharomyces pombe is a widely used unicellular eukaryotic model organism. It is a powerful tractable system used to study various aspects of eukaryotic cellular and molecular biology. However, the knowledge of S. pombe neutral lipids metabolism is quite limited. In this review, we summarize and discuss the current knowledge of the homeostasis of storage lipids and of the role of LD in the fission yeast S. pombe with the aim to stimulate research of lipid metabolism and its connection with other essential cellular processes. We also discuss the advantages and disadvantages of fission yeast in lipid biotechnology and recent achievements in the use of S. pombe in the biotechnological production of valuable lipid compounds.     

采煤机的故障分析与诊断及其发展趋势

分析了采煤机的常见故障,一般出现在机械系统和液压系统;给出了现在常用的故障诊断方法,如温度监测诊断、铁谱分析法、故障诊断专家系统、基于人工神经网络的采煤机故障诊断系统,并指出了它们各自的局限性,再进一步预测了人工神经网络和专家系统相结合的智能故障诊断方法是将来的发展趋势。     

Interaction between the mixing and displacement modes in a naturally ventilated enclosure

Experiments are carried out to study for the first time interactive phenomena in buoyancy-induced natural ventilation in a full-scale enclosure with upper and lower openings on one of the sidewalls. The interaction between the mixing and the displacement ventilation modes is revealed by opening the lower vent to different heights while the upper vent is kept fully open. Both the transient process and steady state interaction are explored. Measurements include temperature differences between inside and outside and air velocity through the upper opening. The level of the neutral plane at the upper vent, defined here as the plane separating between inflow and outflow, decreases with R^*$R^{*}$, the ratio between the opening heights (and areas) of the lower and upper vents. Experiments show that when 0*<0.27$0<R^{*}<0.27$ the mixing and displacement modes interact through a new combined ventilation mode. For 0.53*?1$0.53<R^{*}?1$, the displacement mode dominates whereas in the intermediate range, 0.27?R^*?0.53$0.27?R^{*}?0.53$, either the combined or the pure displacement mode takes place. The experiments are in qualitative agreement with a previous theoretical model.     

Degradation of emergent contaminants by UV,UV/H2O2 and neutral photo-Fenton at pilot scale in a domestic wastewater treatment plant

This study focuses on the removal of 22 selected micropollutants in an effluent from a municipal wastewater treatment plant (MWTP) at pilot scale. A reactor of 37 L with five low pressure mercury lamps emitting at 254 nm (UV₂₅₄) was used. The 22 micropollutants include 15 pharmaceuticals, 2 X-Ray contrast medias, 1 corrosion inhibitor and 4 biocides/pesticides. Five of these 22 compounds were used as indicative substances as proposed by the Swiss Federal Office for the Environment (FOEN) (carbamazepine, diclofenac, sulfamethoxazole, benzotriazole and mecoprop).     

Two industrial examples of coupling experiments and simulations for increasing quality and yield of distilled beverages

The aim of this study was to check the ability of ProSim^® software to model both continuous and batch distillations of two specific industrial units in order to obtain both a better understanding of the behaviour of aroma volatile components and a tool to optimise the still's operation. Simulations of multistage continuous distillation to produce neutral spirit from raw alcohol and of batch distillation to produce bitter orange distillate from bitter orange peels macerate were carried out with ProSimPlus and BatchColumn software. Simulations were compared with distillations performed in two industrial plants. For each case, the industrial plants were studied to determine all the operating parameters and the behaviour of certain compounds selected for their high concentration or quality impact. Then, the NRTL and Henry's law thermodynamic models were chosen. Simulation results of particular compositions of the selected compounds in the different extractions were analysed and compared with experimental measurements. Simulations represented faithfully the behaviour of compounds in the industrial plants. Therefore, it was possible for two totally separated cases to illustrate the interest of simulation software; for neutral spirit production to determine new operation set points in order to maximise productivity and improve quality for neutral spirit production and for bitter orange distillate production, to explain the choice of different cuts and the role of the presence of peels during distillation.     

Progress in the design of the ITER Neutral Beam cell Remote Handling System

The ITER Neutral Beam cell will include a suite of Remote Handling equipment for maintenance tasks. This paper summarises the current status and recent developments in the design of the ITER Neutral Beam Remote Handling System. Its concept design was successfully completed in July 2012 by CCFE in the frame of a grant agreement with F4E, in collaboration with the ITER Organisation, including major systems like monorail crane, Beam Line Transporter, beam source equipment, upper port and neutron shield equipment and associated tooling. Research and development activities are now underway on the monorail crane radiation hardened on-board control system and first of a kind remote pipe and lip seal maintenance tooling for the beam line vessel, reported in this paper.     

Water oxidation by a nickel complex: New challenges and an alternative mechanism

Water splitting toward hydrogen production is a promising way for energy storage. The catalytic role of the metal hydroxides formed by the decomposition of molecular catalysts toward oxygen-evolution reaction (OER) is a challenge in water-splitting systems. Recently, [Ni(TMC)(CH₃CN)](NO₃)₂ (TMC = 1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane, complex 1), was reported to be the most efficient nickel-based complex at neutral pH. In this study, the role of the formed Ni hydroxide-like nanosized species in the presence of this complex under oxygen-evolution reaction (OER) is studied by X-ray photoelectron spectroscopy, scanning electron microscopy, energy dispersive spectrometry, and electrochemical method. A new method for detecting trace Ni hydroxide-like nanosized species on the surface of an electrode is also reported, which is promising for the detection of heterogeneous nickel-based catalysts on the surface of an electrode. The experiments suggest that Ni hydroxide-like nanosized species formed during reaction is an alternative catalyst for OER.     

Improved results on delay-interval-dependent robust stability criteria for uncertain neutral-type systems with time-varying delays

In this paper, we consider the problem of delay-interval-dependent robust stability and stabilization of a class of linear uncertain neutral-type systems with time-varying delay. By constructing a candidate Lyapunov–Krasovskii functional (LKF), that takes into account the delay-range information appropriately, less conservative robust stability criteria are proposed in terms of linear matrix inequalities (LMIs) to compute the maximum allowable upper bounds (MAUB) for the delay-interval within which the uncertain neutral-type system under consideration remains asymptotically stable. The verifiable stabilizability conditions and memoryless state feedback control design are stated. Finally, numerical examples are also designated to demonstrate the effectiveness and reduced conservatism of the developed results.