数字签名技术在医院电子病历中的设计与应用

为推进病历数字化发展,并确保其信息的安全性,将以HIS电子病历系统为基础,采用安信数字签名技术和PKI或PMI系统搭建相信并加以任用的授权服务,经过针对实际的PKC和CA的确认、委托与管控构建整体的数字签名平台,完成电子病历数字签名功能设计,以加强电子文件的完整性、真实性和不可抵赖性。最后以某医院的XML结构化的电子病历系统为基础进行项目实施,完成了医护人员通过HIS的快速身份认证,和准确地数字签名。     

一种标准模型下无证书签名方案的安全性分析与改进

无证书签名具有基于身份密码体制和传统公钥密码体制的优点，可解决复杂的公钥证书管理和密钥托管问题.Wu和Jing提出了一种强不可伪造的无证书签名方案，其安全性不依赖于理想的随机预言机.针对该方案的安全性，提出了两类伪造攻击.分析结果表明，该方案无法实现强不可伪造性，并在"malicious-but-passive"的密钥生成中心攻击下也是不安全的.为了提升该方案的安全性，设计了一个改进的无证书签名方案.在标准模型中证明了改进的方案对于适应性选择消息攻击是强不可伪造的，还能抵抗恶意的密钥生成中心攻击.此外，改进的方案具有较低的计算开销和较短的私钥长度，可应用于区块链、车联网、无线体域网等领域.     

Putative Circulating MicroRNAs Are Able to Identify Patients with Mitral Valve Prolapse and Severe Regurgitation

Mitral valve prolapse (MVP) associated with severe mitral regurgitation is a debilitating disease with no pharmacological therapies available. MicroRNAs (miRNA) represent an emerging class of circulating biomarkers that have never been evaluated in MVP human plasma. Our aim was to identify a possible miRNA signature that is able to discriminate MVP patients from healthy subjects (CTRL) and to shed light on the putative altered molecular pathways in MVP. We evaluated a plasma miRNA profile using Human MicroRNA Card A followed by real-time PCR validations. In addition, to assess the discriminative power of selected miRNAs, we implemented a machine learning analysis. MiRNA profiling and validations revealed that miR-140-3p, 150-5p, 210-3p, 451a, and 487a-3p were significantly upregulated in MVP, while miR-223-3p, 323a-3p, 340-5p, and 361-5p were significantly downregulated in MVP compared to CTRL (p ≤ 0.01). Functional analysis identified several biological processes possible linked to MVP. In addition, machine learning analysis correctly classified MVP patients from CTRL with high accuracy (0.93) and an area under the receiving operator characteristic curve (AUC) of 0.97. To the best of our knowledge, this is the first study performed on human plasma, showing a strong association between miRNAs and MVP. Thus, a circulating molecular signature could be used as a first-line, fast, and cheap screening tool for MVP identification.     

Distributed privacy protection strategy for MEC enhanced wireless body area networks

With the rapid development and widespread application of Wireless Body Area Networks (WBANs), the traditional centralized system architecture cannot handle the massive data generated by the edge devices. Meanwhile, in order to ensure the security of physiological privacy data and the identity privacy of patients, this paper presents a privacy protection strategy for Mobile Edge Computing(MEC) enhanced WBANs, which leverages the blockchain-based decentralized MEC paradigm to support efficient transmission of privacy information with low latency, high reliability within a high-demand data security scenario. On this basis, the Merkle tree optimization model is designed to authenticate nodes and to verify the source of physiological data. Furthermore, a hybrid signature algorithm is devised to guarantee the node anonymity with unforgeability, data integrity and reduced delay. The security performance analysis and simulation results show that our proposed strategy not only reduces the delay, but also secures the privacy and transmission of sensitive WBANs data.     

基于量子秘密共享的盲签名方案

提出了一种基于量子秘密共享的盲签名方案。其中量子秘密共享中用到了Bell纠缠和诱骗光子；盲签名使用的是异或操作和Hash函数。Bell纠缠是纠缠态中最简单的纠缠，而异或操作也是简单易操作；诱骗光子和Hash函数保证了安全性，将这些结合的本方案简单安全易实现，同时还保证了信息的盲性、签名消息的不可否认和不可伪造性。     

<i>In Silico</i> analysis and linking of metabolism-related genes with the immune landscape in head and neck squamous cell carcinoma

Metabolic reprogramming and immunologic suppression are two critical characteristics promoting the progression of head and neck squamous cell carcinoma (HNSCC). The integrative analysis of all the metabolism-related genes (MRGs) in HNSCC is lacking and the interaction between the metabolism and the immune characteristics also requires more exploration to uncover the potential mechanisms. Therefore, this study was designed to establish a prognostic signature based on all the MRGs in HNSCC. Genes of HNSCC samples were available from the TCGA and GEO databases while the MRGs were retrieved from a previous study. Ultimately 4 prognostic MRGs were selected to construct a model possessing robust prognostic value and accuracy in TCGA cohorts. The favorable reproducibility of this model was confirmed in validation cohorts from GEO databases. The risk score calculated by this model was an independent prognostic factor that further classified these HNSCC patients into high-/low-risk groups. GSEA analyses and somatic mutations indicated the low-risk group could activate several anti-tumor pathways and possessed lower TP53 mutation. The results of ESTIMATE, single-sample GSEA, CIBERSORT, and some immune-related molecules analyses suggested the low-risk group exhibited lower metabolic activities and higher immune characteristics. The Spearman correlation test implied most metabolic pathways with tumor-promoting function were negatively correlated with the immune activity, indicating a plausible approach of combining the anti-metabolism and the immunotherapy drugs in the high-risk group to enhance therapeutic effects than applied separately. In conclusion, this prognostic signature linking MRGs with the immune landscape could promote the individualized treatment for HNSCC patients.     

Comparative analysis of inbreeding parameters and runs of homozygosity islands in 2 Italian autochthonous cattle breeds mainly raised in the Parmigiano-Reggiano cheese production region

Reggiana and Modenese are autochthonous cattle breeds, reared in the North of Italy, that can be mainly distinguished for their standard coat color (Reggiana is red, whereas Modenese is white with some pale gray shades). Almost all milk produced by these breeds is transformed into 2 mono-breed branded Parmigiano-Reggiano cheeses, from which farmers receive the economic incomes needed for the sustainable conservation of these animal genetic resources. After the setting up of their herd books in 1960s, these breeds experienced a strong reduction in the population size that was subsequently reverted starting in the 1990s (Reggiana) or more recently (Modenese) reaching at present a total of about 2,800 and 500 registered cows, respectively. Due to the small population size of these breeds, inbreeding is a very important cause of concern for their conservation programs. Inbreeding is traditionally estimated using pedigree data, which are summarized in an inbreeding coefficient calculated at the individual level (F_PED). However, incompleteness of pedigree information and registration errors can affect the effectiveness of conservation strategies. High-throughput SNP genotyping platforms allow investigation of inbreeding using genome information that can overcome the limits of pedigree data. Several approaches have been proposed to estimate genomic inbreeding, with the use of runs of homozygosity (ROH) considered to be the more appropriate. In this study, several pedigree and genomic inbreeding parameters, calculated using the whole herd book populations or considering genotyping information (GeneSeek GGP Bovine 150K) from 1,684 Reggiana cattle and 323 Modenese cattle, were compared. Average inbreeding values per year were used to calculate effective population size. Reggiana breed had generally lower genomic inbreeding values than Modenese breed. The low correlation between pedigree-based and genomic-based parameters (ranging from 0.187 to 0.195 and 0.319 to 0.323 in the Reggiana and Modenese breeds, respectively) reflected the common problems of local populations in which pedigree records are not complete. The high proportion of short ROH over the total number of ROH indicates no major recent inbreeding events in both breeds. ROH islands spread over the genome of the 2 breeds (15 in Reggiana and 14 in Modenese) identified several signatures of selection. Some of these included genes affecting milk production traits, stature, body conformation traits (with a main ROH island in both breeds on BTA6 containing the ABCG2, NCAPG, and LCORL genes) and coat color (on BTA13 in Modenese containing the ASIP gene). In conclusion, this work provides an extensive comparative analysis of pedigree and genomic inbreeding parameters and relevant genomic information that will be useful in the conservation strategies of these 2 iconic local cattle breeds.     

A provable-secure and practical two-party distributed signing protocol for SM2 signature algorithm

Mobile devices are widely used for data access,communications and storage.However,storing a private key for signature and other cryptographic usage on a single mobile device can be challenging,due to its computational limitations.Thus,a number of(t,n)threshold secret sharing schemes designed to minimize private key from leakage have been proposed in the literature.However,existing schemes generally suffer from key reconstruction attack.In this paper,we propose an efficient and secure two-party distributed signing protocol for the SM2 signature algorithm.The latter has been mandated by the Chinese government for all electronic commerce applications.The proposed protocol separates the private key to storage on two devices and can generate a valid signature without the need to reconstruct the entire private key.We prove that our protocol is secure under nonstandard assumption.Then,we implement our protocol using MIRACL Cryptographic SDK to demonstrate that the protocol can be deployed in practice to prevent key disclosure.     

一种云计算数字签名技术的研究与实现

由于云计算具有可靠性高、成本低、性能高等特点,已经成为了新一代信息技术变革的核心。为了能够有效推动互联网安全架构的可持续性发展。本文提出了一种云计算数字签名技术,该技术有机结合了云计算技术与数字签名技术。首先阐述了数字签名系统中应用云计算技术的价值,其次,分析了云计算数字签名技术的实现模型,并且设计了云计算数字签名协议,最后,开展了实例分析,将云计算数字签名技术应用到移动营业厅的业务信息交易中。结果表明：所有业务均可在网上完成,那么既可大大方便用户,又可降低移动营业厅的工作量,还可确保业务信息交易的可靠性、唯一性、真实性、安全性与不可抵赖性。通过结果分析得出结论：云计算数字签名技术既可保障签名文件的完整性与真实性,又可跨平台操作签名文件,还可让数字签名模型利用互联网来对密码运算基础设施进行便捷化、可靠化地访问。     

高效液相色谱-串联质谱法测定婴幼儿配方奶粉中酪蛋白磷酸肽含量

建立一种同位素稀释-高效液相色谱-串联质谱法定量检测婴幼儿配方奶粉中酪蛋白磷酸肽(casein phosphopeptides,CPPs)含量的方法。经Q Exactive Orbitrap扫描,Uniprot蛋白质数据库初步确定CPPs所含肽段,根据质谱响应以及加标回收率等方法学验证实验进一步筛选确定CPPs的特征肽段。样品经过前处理后,采用ACQUITY UPLC BEH 300 C18色谱柱分离,多反应离子监测模式定量测定其中的CPPs特征肽。根据CPPs原料与CPPs特征肽含量的折算系数,从而确定出样品中CPPs的含量。方法学验证结果表明,所建立的方法在10~150 mg/100 g范围内线性关系良好,相关系数大于0.999。在高、中、低3水平加标实验中,回收率在96.2%~100.2%之间,日间重复性在5.3%~7.8%之间。方法定量限为1 mg/100 g,可以满足婴幼儿配方奶粉中不同CPPs含量水平的定量要求。