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1.
目的 构建一种基于时间分辨荧光纳米微球的赭曲霉毒素A (ochratoxin A, OTA)侧流层析试纸条。方法 基于免疫层析原理, 以时间分辨荧光纳米微球为信号探针, 降低非特异性荧光的干扰, 提高检测灵敏度, 并通过优化样品提取液和样品稀释液, 进一步提高现场检测OTA的灵敏度和准确性。结果 OTA在1~50 μg/kg范围内, T线和C线荧光强度的比值与OTA浓度的对数值具有良好的线性关系, 相关系数r2为0.9981~0.9998。不同基质中OTA的检出限(limit of detection, LOD)和定量限(limit of quantitation, LOQ)分别为0.401 μg/kg~0.614 μg/kg和0.970 μg/kg~1.617 μg/kg, 加标回收率为89.53%~118.37%, 相对标准偏差(relative standard deviations, RSDs)小于12% (n=3), 且与呕吐毒素、伏马菌素B1、黄曲霉毒素B1、玉米赤霉烯酮和T-2毒素的交叉反应率均小于5%, 特异性良好。基于荧光定量快速检测技术平台OTA侧流层析试纸条可在8 min内快速准确地定量检测出待测样本中OTA的含量。结论 本研究所制备的时间分辨荧光侧流层析试纸条可实现玉米、小麦和饲料中OTA的快速定量检测, 并具有成本低、灵敏度高、操作简便、准确高、重复性好、特异性好的优点, 可满足国内外OTA检测的技术要求, 为真菌毒素快检技术的发展提供技术支撑。  相似文献   
2.
目标检测技术应用广泛,现有的基于计算机视觉的目标检测方法由于目标遮挡、光照强弱等因素难以适应复杂场景的需求。而人脑的高级认知能力和快速感知能力在处理复杂情况时具有一定的优势。基于事件相关电位(Event-Related Potentials,ERP)的脑机接口(Brain Computer Interface,BCI)系统与特定事件相关,可检测独立于自发脑电的高级认知活动,是当前人工智能领域的研究热点之一。针对基于ERP信号的目标检测各个环节进行了研究现状的全面归纳,梳理了以快速串行视觉呈现(Rapid Serial Visual Presentation task,RSVP)为主的实验范式,包括呈现模式、目标视场角、目标复杂度等设计因素。总结了脑电信号分析中的预处理方法、特征提取和特征分类算法,介绍了其在人脸识别、军事作战、医学分析等领域中的初步应用。探讨了目前研究中存在的问题和挑战并展望未来的研究方向与应用前景。  相似文献   
3.
熔融沉积成型一般以热塑性材料为打印原料,由熔融状态下的熔丝堆积冷却成特定形状。然而熔丝的有效堆积是以下方存在实体模型为基础的,因此需要对熔丝堆积过程中悬空的部分添加支撑结构。首先分析了基于面片倾角的待支撑区域选择方法;然后在切片轮廓的基础上,利用切片分组的思想将三维布尔运算简化处理,降低算法复杂程度,设计了一种新的树状支撑生成算法;根据试验分析支撑参数并封装于算法之中,利用树状支撑算法与其它两种算法进行比较,试验结果表明该算法能有效对悬空区域进行支撑并节省了打印材料和打印时间。  相似文献   
4.
《Ceramics International》2022,48(11):15462-15469
Due to its unique artistic value, mosaic ceramics are widely used in construction-related fields. To meet the artist's demand for high-quality mosaic ceramic to create artistic works, it is necessary to meet the needs for efficient screening of mosaic ceramic tiles. Different from the ordinary large-target ceramics, mosaic ceramics exhibit characteristics of small tile sizes, a variety of colors, large demand for quantities, and easy reflection on the surface. Common manual detection methods show problems of low efficiency or accuracy, easy to fatigue, and many others. To solve these problems, this paper proposes a new detection method to identify surface defects of mosaic ceramic tiles and designs a detection system platform to achieve rapid detection. The experiment proves that the detection system has a detection rate of 93.99% for small defects on the surface of mosaic ceramic tiles, and the detection time of a single mosaic ceramic tile is less than 0.06 s. The detection method can quickly and accurately screen out high-quality, defect-free mosaic ceramic tiles, which can effectively improve the quality and artistic value of mosaic ceramic art creation.  相似文献   
5.
The development of emotional lexicons can be seen as a time- and resource-consuming activity. Several rapid methods have been developed for time-restricted studies, but the suitability of these methodologies in the development of emotional lexicons compared to conventional ones has not been investigated.The aim of this study was to test the suitability of a rapid method, as it is the Sorting Task, for emotional lexicon development compared to a conventional procedure. For this purpose, two different approaches (conventional vs rapid-method) were tested to evaluate wines.222 consumers participated in the lexicon development procedure. A common phase of generation and filtering of the terms was carried out for the development of both lexicons. Then, two approaches were used, one applying the procedures proposed in the literature (conventional, herein as Lexicon I) and the other applying Sorting Task (rapid-method, herein as Lexicon II). To test both lexicons, consumer studies (n = 185) were conducted using a set of seven commercial wines.Both methods were helpful in discriminating emotional responses. Lexicon I reported a wine effect for 10 of the 13 emotional categories, while the Lexicon II discriminated the samples in all 15 categories. Although multifactorial analysis showed a high agreement between configurations (RV = 0.985), the emotional map of both lexicons did not follow the theoretical circumplex model of emotions. SADNESS and NOSTALGIA feelings were related to the second axis.In conclusion, both methods provided similar maps of wines, but Lexicon II was more efficient in terms of time and resources during the lexicon development.  相似文献   
6.
Many places experience extreme temperatures below −30 °C, which is a great challenge for the fuel cell vehicle (FCV). The aim of this study is to optimize the strategy to achieve rapid cold start-up of the 30-cell stack at different temperature conditions. The test shows that the stack rapidly starts within 30 s at an ambient temperature of −20 °C. Turning on the coolant at −25 °C show stability of the cell voltage at both ends due to the end-plate heating, however, voltage of intermediate cells fluctuates sharply, and successful start-up is completed after 60 s. The cold start strategy changes to load-voltage cooperative control mode when the ambient temperature reduced to −30 °C, the voltage of multiple cells in the middle of the stack fluctuate more drastic, and start-up takes 113 s. The performance and consistency of the stack did not decay after 20 cold start-up experiments, which indicates that our control strategies effectively avoided irreversible damage to the stack caused by freeze-thaw process.  相似文献   
7.
唐鍠  王振华 《今日消防》2021,6(7):20-22
执勤车辆是消防装备的核心,具有基础性、平台性、战略性的重要地位,当前人员装备投送方式正由摩托化向机械化转变,同时又向信息化数字化智能化方向发展,所以车辆配备关系到消防救援队伍能否加快转型升级成为有效生成战斗力.文章简要分析了当前消防救援队伍执勤车辆配备的现状发展趋势,结合当今技术发展,论述执勤消防车辆配备可能存在的机遇和挑战.  相似文献   
8.
Si-doped β-Ga2O3 was generally activated by high-temperature annealing (over 600?°C) due to its strong bonding energy. Considering the electronic applications using β-Ga2O3 such as various power devices with low power consumption, it is strongly required to decrease the device process temperature including the impurity activation process. In this article, in order to decrease the impurity activation process temperature, we proposed the rapid thermal annealing (RTA) treatment to activate the Si atoms in the β-Ga2O3 films since RTA treatment can give the high thermal energy to specimen in a short time and investigated the influence of RTA treatment with various temperatures on conductivity activation energy, and structural properties of Si-doped β-Ga2O3 film. Si-doped β-Ga2O3 films were hetero-epitaxially grown on c-plane sapphire substrate by pulsed laser deposition method. Crystallinity, surface roughness, and electrical properties of specimens were investigated by changing the RTA temperatures. Crystallinity and surface roughness of Si-doped β-Ga2O3 films were not significantly influenced by RTA treatment at temperatures range of 100–700?°C. Conductivity activation energy of specimens with RTA treatment was about 50–100?meV and did not depend on RTA temperatures. As a result, even Si-doped β-Ga2O3 film with RTA treatment at 100?°C showed a relatively good conductivity. Based on the experimental results in this study, it can be said that RTA treatment is useful method to decrease the temperature of activation process for Si-doped β-Ga2O3 thin films without serious structural degradations.  相似文献   
9.
目前,对于四川盆地下志留统龙马溪组结核体发育特征及其对黑色页岩段沉积环境指示意义的研究甚少。为此,以该盆地川南—川东坳陷及周缘的露头和钻井资料为基础,通过分析自然伽马、岩石矿物、薄片、地球化学等资料,并结合围岩岩相、地球化学、电性等地质特征的研究结果,探究龙马溪组结核体的发育特征,揭示其对沉积环境的指示意义。研究结果表明:①龙马溪组结核体产出层位与岩相特征区域差异大,结核体层在川南—川东南坳陷区发育于Coronograptus cyphus、Demirastrites triangulatus笔石带中,以钙质硅质混合页岩相、钙质黏土质混合页岩相、黏土质硅质混合页岩相和钙质页岩相为主,在川东—鄂西坳陷区发育于Demirastrites triangulatus、Lituigrapatus convolutus笔石带中,以黏土质硅质混合页岩相、硅质页岩相和钙质硅质混合页岩相为主;②结核体层自然伽马曲线呈低谷响应特征,核体中心富集钙质或硅质,边缘富集黏土质和有机质;③结核体层多赋存于TOC介于0.8%~2.4%、黏土含量超过40%、脆性指数低于50%的碳质页岩和黏土质页岩中,与优质页岩不共生;④鲁丹阶结核体层可作为川南坳陷腹部Coronograptus cyphus带中段对比标志层,埃隆阶结核体层不能单独作为区域对比标志层;⑤结核体主要形成于同沉积—早期成岩阶段,发育于前陆挠曲初期和前陆挠曲发展期,是前陆期深水—半深水陆棚相快速沉积的产物,结核体层的空间分布特征反映出前陆挠曲坳陷自南向北迁移。结论认为,结核体层与笔石、斑脱岩密集段相结合,对于笔石带分层和优质页岩分布研究具有重要的参考价值。  相似文献   
10.
我国的海洋天然气水合物(以下简称水合物)资源主要分布在沿海大陆架水深介于300~3 000 m的深水区,具有弱胶结、非成岩的特征,为了测试和研究该类型水合物必须原位、大量、快速制备样品,而目前常用的搅拌法、喷淋法、鼓泡法等制备技术却存在着生成速度慢、储气密度低等问题。为此,自主设计、研制了1 062 L非成岩水合物快速制备釜,针对我国南海非成岩水合物的物性特征,开展了搅拌法、鼓泡法、喷淋法单一制备方法及“三合一”法(上述3种方法相结合)水合物制备实验,测试了实验过程中温度、压力、电阻率及反应时间等数据,分析比较了几种不同制备方法的水合物生成情况与制备效率。实验结果表明:①搅拌法、鼓泡法、喷淋法制备水合物过程中,生成的水合物均缓慢增加并逐渐铺满整个液面;②搅拌法制备过程中可以观测到明显的诱导期,而喷淋法、鼓泡法及“三合一”法却无明显的诱导期;③单一制备方法及“三合一”法制备水合物过程中,电阻率均随反应时间的增加而增加,其变化趋势亦与水合物制备速率基本一致;④“三合一”法的制备周期明显短于单一制备方法(搅拌法、喷淋法、鼓泡法单一方法的制备时间分别约为“三合一”法的5.14倍、3.59倍、3.16倍)。结论认为,所研制的1 062 L水合物快速制备釜能够实现海洋非成岩水合物样品的原位、快速制备;较之于单一制备方法,“三合一”法大大提高了水合物的制备效率。  相似文献   
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