QuEChERS-GC-MS/MS法同时测定刺梨中15种有机氯农药残留

建立刺梨中15种有机氯农药残留的气相色谱-三重四级杆串联质谱（GC-MS/MS）检测法。刺梨样品采用QuEChERS前处理，正己烷溶液进行提取，经乙二胺-N-丙基硅烷（PSA）和C18各50 mg固相分散净化，浓缩定容后用GC-MS/MS法在多反应监测（MRM）模式下进行测定，通过保留时间、特征离子及其相对丰度定性，外标法定量。15种有机氯农药在0.05～1.00 μg/mL质量浓度范围内呈良好的线性关系，相关系数R2均＞0.997。分别向刺梨样品中分别加入标准品25 μg/kg、50 μg/kg、100 μg/kg时，加标平均回收率在81.98%～105.55%之间，相对标准偏差（n=6）均＜10%，检出限（S/N=3）在0.001 2～0.021 2 mg/kg。该方法具有经济、快速、灵敏度高、重现性好等优势，适用于食品中多种农药残留的同时快速测定。     

超高效液相色谱-串联质谱法测定动物源食品中克霉唑的残留量

目的建立QuEChERS提取和净化、超高效液相色谱-串联质谱(ultraperformanceliquid chromatography-tandem mass spectrometry, UPLC-MS/MS)测定猪肉、牛肉、羊肉、鸡肉等畜禽肉和猪肝、猪肾、羊肝、鸡心等畜禽内脏中克霉唑残留的分析方法。方法样品经正己烷饱和的乙腈提取,提取液经乙腈饱和的正己烷液液分配除脂,并经含PSA和C18粉末的净化管进一步净化。采用0.1%甲酸和乙腈作为流动相进行梯度洗脱,多反应监测模式(multiplereactionmonitoring,MRM)测定,同位素内标法定量。结果本方法在7 min内完成分离分析,方法在0.2～20.0μg/L范围内线性关系良好,相关系数r为0.9999。在0.2、2.0和20μg/kg添加水平下的回收率为78.5%～111.9%,相对标准偏差小于11.1%,方法定量限为0.2μg/kg。结论该方法快速、准确、灵敏,适合动物源食品中克霉唑残留量的测定。     

Compilation of Modern Technologies To Map Genome-Wide Cytosine Modifications in DNA

Over the past few decades, various DNA modification detection methods have been developed; many of the high-resolution methods are based on bisulfite treatment, which leads to DNA degradation, to a degree. Thus, novel bisulfite-free approaches have been developed in recent years and shown to be useful for epigenome analysis in otherwise difficult-to-handle, but important, DNA samples, such as hmC-seal and hmC-CATCH. Herein, an overview of advances in the development of epigenome sequencing methods for these important DNA modifications is provided.     

负径向电场对带电粒子俘获率影响的数值模拟

本文采用数值方法求解Grad-Shafranov方程反演EAST典型长脉冲放电实验(炮号33068)的平衡位形和磁场分布，进而结合粒子在托卡马克电磁场中的运动方程，模拟氘离子在负径向电场存在时的运动轨迹，并统计不同负径向电场下的氘离子俘获率。结果表明：随负径向电场的增大，氘离子轨迹由扩张通行轨迹向外翻香蕉轨迹再向内翻香蕉轨迹，最后向压缩通行轨迹演变；氘离子俘获率随负径向电场的增大而减小，氘离子初始速度越小，其变化越大。     

冶金矿物和产品水分测定问题探讨

水分测定是冶金行业中矿产原料和产品分析的重要内容,主要包括交货水分、湿存水、化合水等测定类别。尽管水分测定方法本身相对简单,但其涉及到矿物原料、产品结算交割以及其他化学分析的校正计算,属于重要的基础性测定方法。各类矿物、产品几乎都有相应水分测定的国家标准、行业标准,部分还是从国外ISO标准体系中采标而来。文章系统介绍并比较各类矿物原料、产品中交货水分、明水、湿存水、化合水等类别的测定方法。由于ISO标准体系中湿存水含义和国内标准体系湿存水含义有较大差异,其测定操作和用途也有不同,使用空气平衡样品和密封原始样品将会得到数据差异较大的湿存水数据,两种湿存水含义需要明确区分。湿存水对化学称样量、灼减量、化合水的测定有影响,需要用湿存水进行水分校正,才能得到更精确的结果。     

The influence of inner material with different average thermal conductivity on the performance of whole insulation system for liquid hydrogen on orbit storage

At present, several composite insulation systems were proposed that can be used for passive insulation systems, including foam-variable density multilayer insulation (VDMLI), aerogel-VDMLI and hollow glass microspheres (HGMs)-VDMLI. The passive insulation systems with different inner material (IM) showed different performances. However, the relationship between the average thermal conductivity of IM and the insulation performance of the whole system has rarely been investigated. It is of great significance for efficient configuration and matching of the passive insulation system. In this paper, a series of average thermal conductivity of IM were assumed to predict the insulation performance of the whole system at 20 K–300 K and high vacuum. In order to further illustrate the relationship between IM and MLI/VDMLI, the foam was replaced by the HGMs as 5 mm a unit forming a series of HGMs-foam-MLI/VDMLI insulation systems. The performance of the systems was investigated. After the foam was completely replaced by the HGMs, the performance of MLI and VDMLI systems was improved 33% and 13%, respectively. Moreover, each mode of heat transfer including solid conduction, radiation, and gas conduction for foam-MLI/VDMLI and HGMs-MLI/VDMLI insulation systems were calculated and analyzed.