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排序方式: 共有437条查询结果,搜索用时 15 毫秒
1.
Johannes Klopf Christine Brostjan Wolf Eilenberg Christoph Neumayer 《International journal of molecular sciences》2021,22(2)
Neutrophils are primary effector cells of innate immunity and fight infection by phagocytosis and degranulation. Activated neutrophils also release neutrophil extracellular traps (NETs) in response to a variety of stimuli. These NETs are net-like complexes composed of cell-free DNA, histones and neutrophil granule proteins. Besides the evolutionarily conserved mechanism to capture and eliminate pathogens, NETs are also associated with pathophysiological processes of various diseases. Here, we elucidate the mechanisms of NET formation and their different implications in disease. We focused on autoinflammatory and cardiovascular disorders as the leading cause of death. Neutrophil extracellular traps are not only present in various cardiovascular diseases but play an essential role in atherosclerotic plaque formation, arterial and venous thrombosis, as well as in the development and progression of abdominal aortic aneurysms. Furthermore, NETosis can be considered as a source of autoantigens and maintains an inflammatory milieu promoting autoimmune diseases. Indeed, there is further need for research into the balance between NET induction, inhibition, and degradation in order to pharmacologically target NETs and their compounds without impairing the patient’s immune defense. This review may be of interest to both basic scientists and clinicians to stimulate translational research and innovative clinical approaches. 相似文献
2.
Magdalena Ostafin Olga Ciepiela Micha Pruchniak Magorzata Wachowska Edyta Uliska Piotr Mrwka Eliza Godkowska-Mrwka Urszula Demkow 《International journal of molecular sciences》2021,22(2)
Acute leukemias, the most common cancers in children, are characterized by excessive proliferation of malignant progenitor cells. As a consequence of impaired blood cell production, leukemia patients are susceptible to infectious complications—a major cause of non-relapse mortality. Neutrophil extracellular traps (NETs) are involved in various pathologies, from autoimmunity to cancer. Although aberrant NETs formation may be partially responsible for immune defects observed in acute leukemia, still little is known on the NET release in the course of leukemia. Here, we present the first comprehensive evaluation of NETs formation by neutrophils isolated from children with acute leukemia in different stages of the disease and treatment stimulated in vitro with phorbol 12-myristate 13-acetate (PMA), N-formyl-methionyl-leucyl-phenylalanine (fMLP), and calcium ionophore (CI). NETs release was measured using quantitative fluorescent method and visualized microscopically. In this setting, NETs release was significantly impaired in leukemic children both at the diagnosis and during the treatment, and full restoration of neutrophil function was achieved only after successful completion of the leukemia treatment. We suggest that neutrophil function impairment may result from both disease- and treatment-related factors. In this context, deficient innate immune response observed in acute leukemia patients may be present regardless of neutrophil count and contribute to secondary immunodeficiency observed in this population. 相似文献
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4.
泌阳凹陷已进入高成熟勘探阶段,开发整体进入特高含水期,可供稳产提效的挖潜空间有限。但泌阳凹陷为富油凹陷,其断层众多砂体变化频繁,形成了许多小型甚至微型的岩性圈闭及断块圈闭,这为油气挖潜提供了地质基础。采用录、测井资料与动态资料相结合,为发现小圈闭油气层提供线索,并进一步研究了小圈闭含油性评价方法,其中包括发现以往各项资料间矛盾层位、应用录井资料与动态资料重新建立测井解释评价标准、判断油藏边界可靠性、圈闭类比发现新的含油圈闭等,分别用于对老区重新进行解释评价,获得了较好的油气挖潜成效。 相似文献
5.
《等离子体科学和技术》2020,22(4):44003
The DC electrical resistivity-temperature characteristic is an important property for insulating materials to operate at a high stress level. In order to improve the DC electrical resistivity at elevated temperature in a targeted way, a positive temperature coefficient(PTC) material(Ba Ti O3-based compound(BT60)) was selected as the filler in this paper, whose electrical resistivity has a PTC effect when the temperature exceeds its Curie temperature. The BT60 was treated with hydrogen peroxide and(3-Aminopropyl) triethoxysilane. Epoxy composites with different loadings of BT60 fillers(0 wt%, 0.5 wt%, and 2 wt% of epoxy) were prepared, denoted as EP-0, EP-0.5, and EP-2. It was shown that BT60 was able to maintain the DC breakdown strength when its loading was less than 2 wt% of epoxy. As the temperature exceeds 60 °C,BT60 will compensate for the negative temperature coefficient effect of epoxy resin to some extent. The electrical resistivity of EP-2 was improved by 55% compared with that of neat epoxy at 90 °C. It was found that the potential barrier at the grain boundary of BT60 and the deep traps in the interface between BT60 and the epoxy resin hinder the migration of carriers and thus increase the electrical resistivity of epoxy composite. 相似文献
6.
高强度不锈钢因其优异的综合性能及成熟的生产工艺,已成为航空、航天、海洋、石化工程等高端制造业领域的重要材料。系统回溯高强度不锈钢的发展及应用历程,总结此类钢的强韧化机理及最新研究进展,并详细梳理了影响该钢的氢陷阱行为及氢脆抗力的主要因素。结合现有研究成果,提出了采用多种类纳米级第二相颗粒复合析出强化突破高强度不锈钢强韧性匹配极限的思路;通过调控钢中析出相及逆转变奥氏体的交互析出行为,提高后者的机械、化学稳定性,使其作为钢中裂纹及可扩散氢的双重“陷阱”,从而提高钢的裂纹及氢脆抗力。最后指出未来新型高强度不锈钢的研发须重点关注以材料基因算法、人工神经网络、机器学习为代表的“人工智能化”合金设计理念。 相似文献
7.
The distribution of Trogoderma granarium Everts has expanded steadily in recent years. This is a very destructive coleopteran species with many economic implications for the trade of grains in countries where it is present. Therefore, it is important to gather data that can confirm or reject the pest’s establishment in a particular country. Spain has been cited as a location where the species is established; however, the present study demonstrates that no actual data support its stablishment in Spain. We surveyed fifteen storage premises (mills and warehouses for animal feed, wheat, and rice) distributed along the Iberian Peninsula over two years. We used traps baited with Trogoderma spp. sex pheromone to conduct the survey. Collected samples were morphologically and molecularly identified, since the morphological identification of this species is difficult. Although we found populations of T. inclusum in most of the sampled premises, and even T. variabile in one of them, no T. granarium was found in any of them. Abundant samples of other coleopterans were captured in the traps, including Oryzaephilus surinamensis, Tribolium spp., Sitophilus spp., Cryptolestes ferrugineus, Lasioderma serricorne and Rhyzopertha dominica. In conclusion, no T. granarium was found in our samplings in Spain. 相似文献
8.
An improved analytical model for the current-voltage (I-V) characteristics of the 4H-SiC metal semiconductor field effect transistor (MESFET) on a high purity semi-insulating (HPSI) substrate with trapping and thermal effects is presented. The 4H-SiC MESFET structure includes a stack of HPSI substrates and a uniformly doped channel layer. The trapping effects include both the effect of multiple deep-level traps in the substrate and surface traps between the gate to source/drain. The self-heating effects are also incorporated to obtain the accurate and realistic nature of the analytical model. The importance of the proposed model is emphasised through the inclusion of the recent and exact nature of the traps in the 4H-SiC HPSI substrate responsible for substrate compensation. The analytical model is used to exhibit DC I-V characteristics of the device with and without trapping and thermal effects. From the results, the current degradation is observed due to the surface and substrate trapping effects and the negative conductance introduced by the self-heating effect at a high drain voltage. The calculated results are compared with reported experimental and two-dimensional simulations (Silvaco®-TCAD). The proposed model also illustrates the effectiveness of the gate-source distance scaling effect compared to the gate-drain scaling effect in optimizing 4H-SiC MESFET performance. Results demonstrate that the proposed I-V model of 4H-SiC MESFET is suitable for realizing SiC based monolithic circuits (MMICs) on HPSI substrates. 相似文献
9.
In the present study, we evaluated the application of mating disruption (MD) for stored-product Pyralidae in a large storage facility of amylaceous products in central Greece. Dispensers were placed in late 2010 until fall 2015, and the stored-product Pyralidae populations were monitored by using pheromone-baited sticky traps and Petri dishes containing semolina were used as oviposition traps. The infesting species that was found was the Mediterranean flour moth, E. kuehniella (Zeller). After the application of MD, the numbers of adult males found on the pheromone-baited traps were reduced in the MD-treated facility, as compared with the untreated (UTC), no MD, facility. Similarly, the numbers of larvae in the oviposition traps were also reduced, as compared with the UTC facility. During the successive years of MD deployment, there was a noticeable decrease in the number of Ephestia kuehniella male adults in the MD-treated facility, but the high density areas, and the infestation foci, were different among years. Hence, in 2014, the majority of the male adults were found at the areas where some years earlier, during the beginning of MD, there were no individuals, or their numbers were low. This suggests that, despite the effectiveness of the method, may be a spatial displacement to other areas. This fact could eventually alter the monitoring protocol, as new infestation foci may occur. 相似文献
10.
Hui Zhang Gang Zhao Yusi Guo Harish Menghwar Yingyu Chen Huanchun Chen Aizhen Guo 《International journal of molecular sciences》2016,17(5)
This study aimed to determine the activity of one Mycoplasma bovis nuclease encoded by MBOV_RS02825 and its association with cytotoxicity. The bioinformatics analysis predicted that it encodes a Ca2+-dependent nuclease based on existence of enzymatic sites in a TNASE_3 domain derived from a Staphylococcus aureus thermonuclease (SNc). We cloned and purified the recombinant MbovNase (rMbovNase), and demonstrated its nuclease activity by digesting bovine macrophage linear DNA and RNA, and closed circular plasmid DNA in the presence of 10 mM Ca2+ at 22–65 °C. In addition, this MbovNase was localized in membrane and rMbovNase able to degrade DNA matrix of neutrophil extracellular traps (NETs). When incubated with macrophages, rMbovNase bound to and invaded the cells localizing to both the cytoplasm and nuclei. These cells experienced apoptosis and the viability was significantly reduced. The apoptosis was confirmed by activated expression of phosphorylated NF-κB p65 and Bax, and inhibition of Iκβα and Bcl-2. In contrast, rMbovNaseΔ181–342 without TNASE_3 domain exhibited deficiency in all the biological functions. Furthermore, rMbovNase was also demonstrated to be secreted. In conclusion, it is a first report that MbovNase is an active nuclease, both secretory and membrane protein with ability to degrade NETs and induce apoptosis. 相似文献