仿真软件在“微机原理与接口技术”教学中的应用

针对＂微机原理与接口技术＂课程内容和特点,介绍了计算机仿真软件：EMU8086、MFS和Proteus ISIS,分别适用于汇编语言程序设计、DOS功能调用和硬件接口技术等不同知识点,并且具有操作简单、界面直观等优点。仿真软件的应用能够改善课堂教学模式,激发学生的学习兴趣,帮助学生更好地掌握理论知识,为实验和实践操作打下良好的基础。     

多频率段物理不可克隆函数

物理不可克隆函数(Physical Unclonable Functions, PUF)是一种用于保护集成电路芯片安全的新方法。传统的基于振荡器的PUF在产生响应过程中振荡器的振荡频率固定不变,因此存在着被攻击的隐患。该文提出一种新的利用多频率段的PUF(Multiple Frequency Slots based PUF, MFS-PUF)来解决这个问题,通过可配置的振荡器,每产生一位响应,振荡器的振荡频率便发生转移。在每一种振荡频率下,由于不可避免地制造差异,振荡器之间的频率会有微小差别,这些略有差异的频率组成了一个频率段(frequency slot),整个系统中则存在着多个频率段。各个频率段之间随机转变,相比于传统的基于振荡器的PUF,系统输入输出响应对(Challenge-Response Pairs, CRPs)的值更大,也更加不可预测,这使得攻击者使用建模攻击的复杂度大大增加,在保证了自身性能的同时增强了本身的安全性。     

多功能结构的支撑技术

多功能结构是航天产品前沿技术。本文首先介绍多功能结构的概念 ,然后讨论多功能结构的支撑技术 ,包括理想材料的分析、设计方法、理想材料零件的设计和制造方法、微电子元件的构型优化以及多功能结构设计方法。针对多功能结构设计 ,提出功能驱动的设计框架 ,及基于博弈论的设计方程 ,并给出一个建立设计方程的实例     

Modeling membrane proteins based on low-resolution electron microscopy maps: a template for the TM domains of the oxalate transporter OxlT

The availability of both EM and high-resolution crystallographic data for several membrane proteins (MPs) permits a detailed evaluation of the ability of molecular modeling techniques to complement EM data in the development of models of MPs. A protocol for this purpose is presented, consisting of (1) identifying transmembrane (TM) domains from sequence; (2) assigning buried and lipid-exposed faces of the TM domains; and (3) assembling the TM domains into a bundle, based on geometric restraints obtained from the EM data. The protocol is validated by predicting the structures of several 7- and 12-TM MPs to within 3-5 A r.m.s.d. from their crystal structures. The protocol is applied to generate a model of the oxalate transporter OxlT, for which a high-resolution structure is not yet available.     

Biochemistry of bacterial multidrug efflux pumps

Bacterial pathogens that are multi-drug resistant compromise the effectiveness of treatment when they are the causative agents of infectious disease. These multi-drug resistance mechanisms allow bacteria to survive in the presence of clinically useful antimicrobial agents, thus reducing the efficacy of chemotherapy towards infectious disease. Importantly, active multi-drug efflux is a major mechanism for bacterial pathogen drug resistance. Therefore, because of their overwhelming presence in bacterial pathogens, these active multi-drug efflux mechanisms remain a major area of intense study, so that ultimately measures may be discovered to inhibit these active multi-drug efflux pumps.     

PZT Based MFS Structure for FeFET

Fabrication and properties of lead zirconate titanate (PZT) thin films have been studied for Metal-Ferroelectric-Semiconductor FET (MFSFET) devices. PZT based MFS capacitors using lead titanate (PT) as seeding layers have been respectively prepared on p-type ?111? and n-type ?100? silicon wafers directly by a sol-gel method. PZT/PT films are final annealed at 650°C for 1 min in oxygen ambient using rapid thermal annealing (RTA). The measured memory windows of the MFS capacitors are about 1.8 V and 5 V under the polarization voltages of ±5 V and ±10 V correspondingly. The MFS structure can be valuable for MFSFET applications.     

基本解方法解水下刚性目标三维Helmholtz外散射问题

运用基本解方法求解水下刚性目标三维Helmholtz外散射问题。研究了源点位置分布和数目对基本解方法计算结果的影响,比较了最小二乘配点法和等额配点法的计算精度。结果表明,当源点构成的形状与目标边界的形状差异大时,计算精度差,增加源点的数量可提高计算精度,运用较少的源点也可获得令人满意的精度,从而提高计算效率,但源点不宜距离目标边界过远;最小二乘配点法的计算精度较等额配点法高些。     

YMnO_3薄膜的铁电性能及其应用

对金属 -铁电体 -半导体场效应晶体管器件而言 ,具有六方晶系结构的稀土锰酸盐 ( Re Mn O3)是性能优良的薄膜材料 ,它们的介电常数低 ,仅仅只有单一的极化轴 ,没有挥发性的元素 Pb、Bi等。本文作者对 Re Mn O3材料的结构特征、制备方法及其铁电性能等进行了介绍 ,并指出存在的困难及其发展方向。     

The mei3 region of the Schizosaccharomyces pombe genome

Expression of the mei3 gene is sufficient to induce meiosis in the fission yeast Schizosaccharomyces pombe. The mei3 gene is located 0.64 Mb from the telomere of the left arm of Sz. pombe chromosome II. We have sequenced and analysed 107 kb of DNA from the mei3 genomic region. The sequence includes 14 known genes (bag1-B, csh3, dps1, gpt1, mei3, mfm3, pac1, prp31, rpl38-1, rpn3, rti1, spa1, spm1 and ubc4) and 26 other open reading frames (ORFs) longer than 100 codons: a density of one protein-coding gene per 2.7 kb. Twenty-one of the 40 ORFs (53%) have introns. In addition there is one lone Tf1 transposon long terminal repeat (LTR), tRNA(Trp) and tRNA(Ser) genes and a 5S rRNA gene. 14 of the novel ORFs show sequence similarities which suggest functions of their products, including a coatomer alpha-subunit, a catechol O-methyltransferase, protein kinase, asparagine synthetase, zinc metalloprotease, acetyltransferase, phosphatidylinositol 4-kinase, inositol polyphosphate phosphatase, GTPase-activating protein, permease, pre-mRNA splicing factor, 20S proteasome component and a thioredoxin-like protein. One predicted protein has similarity to the human Cockayne syndrome protein CSA and one with human GTPase XPA binding protein XAB1. Three ORFs are likely to code for proteins because they have sequence similarity with hypothetical proteins, three encode predicted coiled-coil proteins and four are sequence orphans.     

New Multidrug Efflux Systems in a Microcystin-Degrading Bacterium Blastomonas fulva and Its Genomic Feature

A microcystin-degrading bacterial strain, Blastomonas fulva T2, was isolated from the culture of a microalgae Microcystis. The strain B. fulva T2 is Gram-stain-negative, non-motile, aerobic, non-spore-forming and phototrophic. The cells of B. fulva T2 are able to grow in ranges of temperature from 15 to 37 °C, with a pH of 6 to 8 and a salinity of 0 to 1% NaCl. Here, we sequenced the complete genome of B. fulva T2, aiming to better understand the evolutionary biology and the function of the genus Blastomonas at the molecular level. The complete genome of B. fulva T2 contained a circular chromosome (3,977,381 bp) with 64.3% GC content and a sizable plasmid (145.829 bp) with 60.7% GC content which comprises about 3.5% of the total genetic content. A total of 3842 coding genes, including 46 tRNAs and 6 rRNAs, were predicted in the genome. The genome contains genes for glycolysis, citric acid cycle, Entner–Doudoroff pathways, photoreaction center and bacteriochlorophylla synthesis. A 7.9 K gene cluster containing mlrA, mlrB, mlrC and mlrD_1,2,3,4 of microcystin-degrading enzymes was identified. Notably, eight different efflux pumps categorized into RND, ABC and MFS types have been identified in the genome of strain T2. Our findings should provide new insights of the alternative reaction pathway as well as the enzymes which mediated the degradation of microcystin by bacteria, as well as the evolution, architectures, chemical mechanisms and physiological roles of the new bacterial multidrug efflux system.