Optimized Biocatalytic Synthesis of 2-Selenopyrimidine Nucleosides by Transglycosylation**

Selenium-modified nucleosides are powerful tools to study the structure and function of nucleic acids and their protein interactions. The widespread application of 2-selenopyrimidine nucleosides is currently limited by low yields in established synthetic routes. Herein, we describe the optimization of the synthesis of 2-Se-uridine and 2-Se-thymidine derivatives by thermostable nucleoside phosphorylases in transglycosylation reactions using natural uridine or thymidine as sugar donors. Reactions were performed at 60 or 80 °C and at pH 9 under hypoxic conditions to improve the solubility and stability of the 2-Se-nucleobases in aqueous media. To optimize the conversion, the reaction equilibria in analytical transglycosylation reactions were studied. The equilibrium constants of phosphorolysis of the 2-Se-pyrimidines were between 5 and 10, and therefore differ by an order of magnitude from the equilibrium constants of any other known case. Hence, the thermodynamic properties of the target nucleosides are inherently unfavorable, and this complicates their synthesis significantly. A tenfold excess of sugar donor was needed to achieve 40−48 % conversion to the target nucleoside. Scale-up of the optimized conditions provided four Se-containing nucleosides in 6–40 % isolated yield, which compares favorably to established chemical routes.     

磨料有序排布电镀砂轮的制备及其把持力研究

为解决电镀砂轮磨削加工中容屑空间不足的问题，采用点胶微粘接的方法制备了磨料有序排布的电镀砂轮，分析了磨料粘接效果和镀层力学性能。通过SEM分析了磨料/镀层/导电胶的结合界面，并进行了干磨削试验。研究结果表明，直径约为磨料粒径40%的胶点可粘接住磨料，单个胶点上粘接多颗磨料的占比小于6%；双脉冲电镀工艺制备的镀层显微硬度大于500HV，表层残余应力小于100MPa，磨料/镀层/导电胶之间的界面贴合紧密，无明显缺陷；砂轮在磨削时没有出现磨料脱落现象。     

Large eddy simulation of flow over square cylinder arrays in an octagonal configuration at subcritical Reynolds numbers

In this study, fluid flow over an array of eight, 0.029 m × 0.029 m, square cross‐section cylinders in an octagonal configuration is studied numerically. The mean force coefficients (drag and lift) and the vortex formation characteristics of the array are calculated numerically by utilizing a three‐dimensional large eddy simulation mathematical model for turbulence. The numerical simulation is performed with commercial software ANSYS Fluent 19R1. To investigate the parametric influences, three spacings between the cylinders (0.07, 0.14, and 0.2 m), two array attack angles (0° and 15°), and two Reynolds numbers (4060 and 45 800) are considered. The results comprise flow patterns and force coefficients' variations with Reynolds numbers. The lift force of the downstream cylinder reaches its maximum at α = 15°, and the drag force of the upstream cylinders finds its peak at α = 0°. It is observed through velocity and viscosity contour plots that vortex formation length near the cylinder increases at higher Reynolds number. Velocity vector plots are also presented to show fluid flow behavior near the cylinder. Furthermore, the predicted mean forces on the cylinders are slightly different for different Reynolds numbers, spacings, and angles of attack.     

高校网络安全建设的探索与思考

The construction of cybersecurity is an important part of the information construction in university. It is an indispensable condition to help the information construction and a strong guarantee to serve the information support of teachers and students in uni- versity. The cybersecurity environment, atmosphere and guarantee ability of university need to be improved. Based on the related work and achievements of cybersecurity construction, some general schemes are put forward to provide reference for cybersecurity construction in university.     

交错巷道巷间围岩承载结构稳定性分析

针对普遍存在的矿井交错巷道围岩体稳定性及支护问题,运用极限平衡分析和弹塑性力学建立交错巷道巷间围岩稳定性分析判别方程,探讨了交错巷道巷间围岩稳定性主要影响因素,并进行了工业性试验。结果表明:交错巷道巷间围岩体受围岩水平应力集中叠加影响,围岩破坏自巷间围岩体上下表面向中部延伸,加之巷间围岩体尺寸有限,一旦破坏贯通将致使巷间围岩承载存在失稳隐患;交错巷道巷间距越小,越近乎平行布置都会加剧巷间围岩破坏且诱发巷间围岩破坏贯通,合理设计断面尺寸及下方巷道合理支护强度可有效控制并维持围岩稳定;现场采用交错巷道联合支护方案后,交错位置上巷道断面完整无较明显变形且下巷道围岩最大变形量小于100 mm,支护效果显著。     

Numerical investigation of hydrogen absorption in a stackable metal hydride reactor utilizing compartmentalization

In this paper, a three-dimensional model for hydrogen absorption in a metal alloy has been developed, validated against the experimental data in the literature, and then applied to a novel design for a hydrogen storage unit. The proposed design is similar to the fuel cell stack, but here the Membrane Electrode Assembly (MEA) has been replaced by a metal hydride (MH) reactor placed between the flow-field plates. These are stacked together to achieve the required amount of hydrogen storage. The flow-field plates have channels engraved on one side for hydrogen supply and on the other, for coolant/heating medium. It is known that the effectiveness of a hydrogen storage unit is directly related to its heat transfer area, and therefore, the choice of its geometry is very important. The larger the size, the more the resistance to heat transfer. Although, the internal tubular heat exchangers have proven to be effective in heat transfer, they pose severe challenges such as cooling/heating medium leakage due to tube erosion, stresses generated, etc. and they displace the active metal hydride from the tank. The present stacked MH reactor configuration helps to overcome these challenges by stacking small MH reactors together and there is no chance of the cooling/heating medium leaking into the metal hydride. Numerical simulations were performed to investigate the effect of coolant flow rate and percentage of flow-field plate rib area exposed to the MH reactor on temperature evolution and the amount of hydrogen stored. Further, a detailed study was carried out to understand the effect of compartmentalization of the MH reactor on temperature distribution. The results revealed that compartmentalization substantially helps to uniformly distribute the temperature in the metal bed, which is very important to maintain uniform utilization of the metal powder. Consequently, the uniform metal powder density for repeated absorption-desorption cycles without significant loss of its hydrogen storage capabilities.     

风洞模型加速度负反馈振动主动控制方法研究

针对尾撑式风洞模型强方向性振动问题,基于异位配置加速度负反馈控制器对风洞模型振动主动控制方法进行研究。首先,通过模态分析得到系统低阶模态振动的强方向性并基于系统特性设计了具有结构耦合性的内嵌压电陶瓷作动器的风洞模型振动主动控制系统。然后,基于异位配置NAF控制器分别设计了针对第二阶模态和前两阶模态的单模态NAF控制算法和双模态NAF控制算法。最后,进行了实验验证,结果表明:双模态NAF控制算法控制效果较好,前两阶模态阻尼比分别提高近13倍和近40倍,镇定时间分别缩短近11s和近26s。     

油田含CO2伴生气变压吸附材料实验研究

变压吸附工艺是国内小规模CO_2驱产出气处置的常用手段,而吸附剂是吸附分离的基础。选用6种沸石类吸附剂,测试了CO_2/CH4单组分吸附平衡等温线,分析了温度对吸附量的影响。优选了两种材料进行了CO_2-CH4混合气体竞争吸附实验,重点分析了温度、压力、气体组分、CO_2含量等因素对吸附剂吸附分离效果的影响,评价了油、水含量对吸附剂的损伤。实验结果表明:吸附剂的分离系数与混合气体中CO_2含量无明显相关性,但吸附体系压力的变化对混合气体分离效果影响较大。在初始气体CO_2摩尔分数50%、温度20℃、压力2.5MPa条件下,优选的吸附剂13X对混合气体的分离系数为25。油水的存在会对吸附剂的分离性能产生较大影响,在温度20℃、压力2.5 MPa、13X吸附剂含水摩尔分数10%及含油摩尔分数10%条件下,吸附总量分别下降32%和78%,分离系数分别下降48%和78%。     

Integrated multi-factory production and distribution scheduling applying vehicle routing approach

This paper introduces a new integrated multi-factory production and distribution scheduling problem in supply chain management. This supply chain consists of a number of factories joined together in a network configuration. The factories produce intermediate or finished products and supply them to other factories or to end customers that are distributed in various geographical zones. The problem consists of finding a production schedule together with a vehicle routing solution simultaneously to minimise the sum of tardiness cost and transportation cost. A mixed-integer programming model is developed to tackle the small-sized problems using CPLEX, optimally. Due to the NP-hardness, to deal with medium- and large-sized instances, this paper develops a novel Improved Imperialist Competitive Algorithm (IICA) employing a local search based on simulated annealing algorithm. Performance of the proposed IICA is compared with the optimal solution and also with four variants of population-based metaheuristics: Imperialist Competitive Algorithm, Genetic Algorithm, Particle Swarm Optimisation (PSO), and Improved PSO. Based on the computational results, it is statistically shown that quality of the IICA’s solutions is the same as optimal ones solving small problems. It also outperforms other algorithms in finding near-optimal solutions dealing with medium and large instances in a reasonably short running time.