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1.
Sobha Karuthedom George Lucia Laukov Ren Weiss Vladislav Semak Birgit Fendl Victor U. Weiss Stephanie Steinberger Günter Allmaier Carla Tripisciano Viktoria Weber 《International journal of molecular sciences》2021,22(8)
Growing interest in extracellular vesicles (EVs) has prompted the advancements of protocols for improved EV characterization. As a high-throughput, multi-parameter, and single particle technique, flow cytometry is widely used for EV characterization. The comparison of data on EV concentration, however, is hindered by the lack of standardization between different protocols and instruments. Here, we quantified EV counts of platelet-derived EVs, using two flow cytometers (Gallios and CytoFLEX LX) and nanoparticle tracking analysis (NTA). Phosphatidylserine-exposing EVs were identified by labelling with lactadherin (LA). Calibration with silica-based fluorescent beads showed detection limits of 300 nm and 150 nm for Gallios and CytoFLEX LX, respectively. Accordingly, CytoFLEX LX yielded 40-fold higher EV counts and 13-fold higher counts of LA+CD41+ EVs compared to Gallios. NTA in fluorescence mode (F-NTA) demonstrated that only 9.5% of all vesicles detected in scatter mode exposed phosphatidylserine, resulting in good agreement of LA+ EVs for CytoFLEX LX and F-NTA. Since certain functional characteristics, such as the exposure of pro-coagulant phosphatidylserine, are not equally displayed across the entire EV size range, our study highlights the necessity of indicating the size range of EVs detected with a given approach along with the EV concentration to support the comparability between different studies. 相似文献
2.
Tracking-by-detection (TBD) is a significant framework for visual object tracking. However, current trackers are usually updated online based on random sampling with a probability distribution. The performance of the learning-based TBD trackers is limited by the lack of discriminative features, especially when the background is full of semantic distractors. We propose an attention-driven data augmentation method, in which a residual attention mechanism is integrated into the TBD tracking network as supplementary references to identify discriminative image features. A mask generating network is used to simulate changes in target appearances to obtain positive samples, where attention information and image features are combined to identify discriminative features. In addition, we propose a method for mining hard negative samples, which searches for semantic distractors with the response of the attention module. The experiments on the OTB2015, UAV123, and LaSOT benchmarks show that this method achieves competitive performance in terms of accuracy and robustness. 相似文献
3.
气藏平均地层压力跟踪计算新方法 总被引:1,自引:0,他引:1
平均地层压力是产能评价和动态分析的基础,准确、快速获取平均地层压力对高效开发气藏意义重大。基于地层压力随时间变化的规律,分析了平均地层压力的变化规律。研究结果表明:平均地层压力等效点仅随时间发生改变,平均地层压力的下降速率等于或者近似等于井底流压的下降速率。从封闭弹性驱动气藏的物质平衡方程出发,考虑偏差系数和井底流压随平均地层压力的变化,推导建立了平均地层压力跟踪计算新方法,根据生产数据可迭代计算平均地层压力。方法验证结果显示,采气速度和采出程度共同影响模型的计算结果。应用实例表明,跟踪计算法与压力恢复试井和物质平衡法之间的相对误差均较小,满足工程计算精度要求,且跟踪计算法不需依托生产测试数据,节约了测试费用,避免了测试占产。 相似文献
4.
目的 针对“新国潮”这一新兴的设计现象进行受众关注度研究,旨在剖析“新国潮”设计风格,发现影响“新国潮”受众关注度的设计要素,并展开实验分析,为设计领域的实证研究提供方法层面上的启示,为“新国潮”风格的推广提供设计策略。方法 在从字体、符号和色彩三方面解构“新国潮”设计要素的基础上,通过因素分析方法确定实验数据的信度、效度,运用眼动实验系统和方差分析法从无意注意和有意注意两个阶段实证研究“新国潮”设计要素对受众关注度的影响,并对原因展开分析。结果 在无意注意阶段,书法体、有色彩渐变、高纯度色彩、非象征符号能够获得较高关注度;在有意注意阶段,受众对宋体、有色彩渐变、高纯度色彩、象征符号的关注度更高。结论 研究在提高受众对“新国潮”画面的关注度、发现关注度影响机理和构建设计要素有效表达方面具有重要意义。 相似文献
5.
This paper aims to provide a review of the conceptual design and theoretical framework of the main control schemes proposed in the literature for unmanned underwater vehicles (UUVs). Additionally, the objective of the paper is not only to present an overview of the recent control architectures validated on UUVs but also to give detailed experimental-based comparative studies of the proposed control schemes. To this end, the main control schemes, including proportional–integral–derivative (PID) based, sliding mode control (SMC) based, adaptive based, observation-based, model predictive control (MPC) based, combined control techniques, are revisited in order to consolidate the principal efforts made in the last two decades by the automatic control community in the field. Besides implementing some key tracking control schemes from the classification mentioned above on Leonard UUV, several real-time experimental scenarios are tested, under different operating conditions, to evaluate and compare the efficiency of the selected tracking control schemes. Furthermore, we point out potential investigation gaps and future research trends at the end of this survey. 相似文献
6.
《International Journal of Hydrogen Energy》2022,47(92):39239-39254
The energy management and trajectory tracking control are crucial to realize long-endurance autonomous flight for hybrid electric UAVs. This study aims to comprehensively consider energy management and trajectory tracking for hybrid electric fixed wing UAVs with photovoltaic panel/fuel cell/battery. A double-layer fuzzy adaptive nonlinear model predictive control method (DFNMPC) is proposed. Separated by the surplus demand power, energy management and trajectory tracking problem are decoupled into the high-layer fuzzy adaptive nonlinear model predictive controll problem (H-FNMPC) and low-layer fuzzy adaptive nonlinear model predictive controll problem (L-FNMPC). H-FNMPC solves the trajectory tracking and navigation control probelm for the greatest benefit of solar energy. L-FNMPC solves the power allocation problem of hybrid energy system for minimum equivalent hydrogen consumption. A fuzzy adaptive prediction horizon adjustment method based on UAV maneuvering degree is proposed to effectively improve proposed method adaptability to different mission profiles. Analogously, a fuzzy adaptive equivalent hydrogen consumption factor adjustment method in L-FNMPC is proposed to ensure the flexible utilization of battery. In addition, an equivalent hydrogen flow rate calculation method based on the real-time current ratio is proposed for PV/FC/Battery hybrid energy system. Numerical simulation results including a spiral trajectory tracking and a quadrilateral trajectory tracking, demonstrate that DFNMPC can simultaneously handle energy management and trajectory tracking problem for hybrid electric UAVs. Compared to hierarchical fuzzy state machine strategy, DFNMPC can save 13.3% hydrogen for the spiral trajectory tracking, and 56.9% for the quadrilateral trajectory tracking. It indicates that the energy efficiency can be improved from both levels of energy management and flight motion. The proposed method prospected for exploring high-energy-efficiency autonomous flight of hybrid electric UAVs in the future. 相似文献
7.
《Contact lens & anterior eye》2022,45(5):101497
PurposeThe assessment of symptoms of dry eye disease using a questionnaire is an effective and simple method of quantifying symptoms. The aim of this study was to translate the SPEED questionnaire and adapt it for the Italian language and verify the main psychometric performance of the translated version, including repeatability and agreement.MethodsThe SPEED questionnaire was translated into Italian following a standard methodology. The resulting questionnaire was administered to 206 adult participants in order to perform a validation analysis. A subgroup of 82 participants was retested after one week to give a repeatability and agreement assessment.ResultsInternal consistency showed an alpha of 0.852 (95% CI 0.818–0.881) and no unnecessary items. The factor analysis showed a saturation for three main factors related to (i) Dryness and Soreness, (ii) Fatigue, and (iii) Burning. Repeatability was high, with a CCC of 0.896 (95% CI 0.844–0.931). Agreement analysis showed no significant bias between sessions and an interval of agreement of 5 points for SPEED score.ConclusionThe translation and adaptation of the SPEED questionnaire for the Italian language have demonstrated good psychometric properties for the translated questionnaire, confirming and expanding the original psychometric characteristics. Consequently, the SPEED questionnaire could be used to measure the presence of symptoms of dry eye quantitatively. 相似文献
8.
为了缩小增强后环焊缝图像质量主客观评价的差距,提出了一种基于人眼视觉的无参考质量评价指标。通过分析人眼的视觉特性,建立图像模糊评价模型,设定主观评价方法;主客观评价值进行曲线拟合,经验证,评价结果与主观评价结果具有线性拟合关系,评价结果接近人眼最佳视觉,验证了评价指标的可行性。 相似文献
9.
Liyan Wen Gang Tao Bin Jiang 《International Journal of Adaptive Control and Signal Processing》2021,35(3):373-400
In this article, adaptive compensation designs are developed for nonlinear systems with uncertainties from the system functions and persistent actuator failures of characterizations that (i) some unknown system inputs are stuck at some unknown fixed or varying values at unknown time instants and (ii) the failure pattern always switches from one to another and the switching does not stop. Such a controlled plant is described by an uncertain time-varying nonlinear system, and some robust adaptive feedback linearization based failure compensation results are studied for closed-loop system stabilization and bounded output tracking for some specific conditions. To improve the tracking performance in the presence of persistent actuator failures, a new adaptive control scheme is developed, using the failure indicator function which contains the failure pattern and failure time in the formulation. Detailed stability and tracking performance are shown. Simulation results are shown to verify the effectiveness of the proposed adaptive actuator failure compensation method. 相似文献
10.
《Mechatronics》2021
Producing a stable and agile bionic eye for visual image acquisition in robotics is a challenging task. In this paper, we design a bionic eye with mirror-symmetric distribution and cross-connection of flexible ropes. This mechanism is based on oculomotor law and the physiological structure of the extraocular muscles (EOMs). Specifically, the basic structural parameters are determined by Listing’s law, and the unique connection of the flexible ropes can realize the functions of the recti and oblique muscles. Furthermore, to mimic the trochlea structure, a pulley mechanism is constructed to permit the free movement of the flexible ropes. Through simulation and physical experiments, it is demonstrated that the bionic eye mechanism can move with agility under the structural parameters. The experimental results indicate that the proposed bionic eye mechanism has a superior motion accuracy of 2.798 mm, which is 6.7% of the maximum motion distance, and the repeatable accuracy of the mechanism can up to 0.210 mm. 相似文献