Isolation and preliminary characterization of Pichia pinus mutants insensitive to glucose repression

A new method for the isolation of glucose repression-insensitive mutants in the methylotrophic yeast Pichia pinus was developed. The method is based on screening of small suspension samples derived from 2-deoxyglucose-resistant colonies for alcohol oxidase activity. Alcohol oxidase activity was evaluated by determination of formaldehyde excreted by cells. Mutants with glucose non-repressible alcohol oxidase and catalase synthesis were obtained. All mutants grew poorly on D -xylose compared to the wild type, whereas growth on L -arabinose was similar to the wild type. Changes in the glucose transport system were suggested to be responsible for altered growth characteristics and defective glucose repression.     

Isolation of single yeast cells by optical trapping

Individual yeast cells can be successfully isolated and recultured on plates with a new isolation method making use of optical trapping with infrared laser light. The cells can be selected on morphological criteria by high resolution microscopy. The isolation device is constructed from two coverslips separated by spacers, in which selected cells are transferred to a plastic capillary, using the optical trap. To test the procedure, selection experiments were done with a mixture of two Saccharomyces cerevisiae strains, distinguishable both in fluorescence microscopy and on agar plates. These experiments showed that only selected cells were isolated, and close to 100% of the isolated stationary-phase cells formed colonies on agar plates, indicating a high recovery. A lower recovery was obtained with exponential-phase cells, possibly because of a higher sensitivity to laser irradiation. Applications for this method may include the isolation of mutants with altered morphology and the isolation of subpopulations of yeast cultures, for their separate investigation or for the initiation of pure cultures.     

An MAS Framework for Speculative Trading Research in Stock Index Futures Market

In this paper, we develop a futures trading simulation system to determine how speculative behavior affects the futures mar ket. A eonfigurable client is designed to simulate traders, and users can define trade strategies using different programming languages. A lightweight server is designed to handle large scale and highly eoncmTent access requests from clients. HBase is chosen as the database to grantee sealability of the system. As HBase only supports single row transaction, a transaction support mechanism is developed to improve data consistency for HBase. The HBase transaction support mecha nism supports multirow and multitaMe by using two phase commit protocol. The experiments indicate that our system shows high efficiency in the face of the large scale and high concurrency access request, and the read/write performance loss of HBase introduced by the transaction support mecha nisms is also acceptable.     

网间安全隔离技术分析研究

随着互联网的普及和电子政务在日常工作、学习和生活的普遍使用,网络信息安全的重要性愈发引人关注,保证网络信息的安全已成为推进社会网络化的重点,网络隔离技术也成为了网络安全技术发展的一个关注点。文中系统地分析了现有的网络隔离技术现状及不足,并提出一种新的基于可信计算技术的网间安全隔离方案。与传统的方案相比,新方案不仅更加简洁、灵活,同时较大提高了系统的安全性。     

临近空间卫星通信天线伺服跟踪的研究

在临近空间飞行器卫星通信系统中,为了隔离天线载体姿态变化对天线指向的扰动,天线伺服控制需采取波束稳定和跟踪措施,典型的步进跟踪、惯导引导跟踪及单脉冲跟踪体制都难以满足飞行器长期滞空的使用要求,需要研究一种新的跟踪方法。通过分析,给出了相控波束倾斜跟踪方案。采用相控技术使波束产生倾斜和圆锥扫描,实现了高精度的圆锥扫描跟踪,具有独立性好、不需校相等优点,能够适应临近空间飞行器卫星通信天线长期滞空的跟踪要求。     

Extension of the bond graph causality inversion method for fault detection and isolation

Controlled systems can be subjected to faults that may affect the performance of the system, and unable its objectives to be achieved. Fault detection and isolation algorithms are then used to study these faults. The bond graph tool can be used for modeling purposes and then its structural, and causal properties can be exploited for automatic generation of analytical redundancy relations (ARRs) through a procedure named causality inversion method, which are used for diagnosis applications. These ARRs are mathematical constraints that are used to verify the coherence between the process measurements and the system model. This paper proposes an extension of the causality inversion method by different versions of the same ARR. The goal is to increase the number of isolable faults. Moreover, structural conditions are given in order to avoid the generation of redundant ARRs. To validate the obtained structural procedure, a fault is imposed in a traction of an omnidirectional mobile robot.     

K波段环形平衡混频器的仿真设计

针对在毫米波段,由于波长较短,对3dB电桥结构来说,每臂太短且太宽,使电桥难于实现;而对环形结构来说,环的特性阻抗高,微带线窄,环的周长大,达到制造误差和设计误差都较小的目标。本文提出了一种K波段环形平衡混频器的设计方法,采用ADS的co-simulation工具对其进行了仿真,重点分析了隔离度、变频损耗和线性度方面的问题。文中给出了仿真测试数据,对数据进行了分析。并根据仿真结果可以得出该混频器具有较好的隔离度,变频损耗和线性度。     

Feedback supported isolation structure for blocking of minority leakage carriers in power integrated circuits

A combination of two conventional junction isolation structures is used to produce a device, which significantly improves the blocking of minority carriers injected into the substrate of a power IC due to switching of an inductive load. Simulation results show that the connection scheme employed greatly enhances the efficiency of the structures. A substrate current reduction of up to four orders of magnitude compared to conventional junction isolation structures is achieved. The significance of doping profiles in the p-sinker region is evaluated.     

RS-422/485隔离技术及应用讨论

概述了RS-422/485串行总线技术标准,并对工业应用现场中可能出现的影响总线误判的因素进行了分析,对目前使用的隔离技术进行对比和总结。最后给出了实际的解决方案。     

中性线的转换对UPS性能的影响和对策

由于低压配电系统接地故障保护（GFP）的需要,市电与备用发电机组电源转换电路必须采用4极自动转换开关（ATS）,而有些4极ATS在转换过程中存在中性线中断现象,严重影响了UPS的正常运行,由此引发的UPS故障和负载停电事件越来越受到关注。文中分析电信和数据中心常用市电和备用发电机组电源转换电路的性能及其对GFP和UPS运行的影响,提出工程实用解决方案。