Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

“锲而不舍 金石可镂”——上海秀派公司2．45GHz有源RFID标签技术的成功之路

上海秀派公司坚持自己的技术长项——2．45GHz有源RFID,充分发扬该种RFID的特长,记者采访该公司时了解到了开发方面的很多细节、成功案例和实施经验。我们不但非常推崇该公司的“锲而不舍”的意志,而且还意识到如果秀派公司的成功经验一旦辐射到中国的RFID行业中,一定会把这项在国外诞生的技术更好地植根于中国的土壤,使之更加适合中国的实用环境。     

一种动态Q值多标签识别算法的性能分析

EPCglobal组织发布的第二代超高频射频识别空中接口协议EPC-C1G2协议可同时识别多个标签,该协议采用时槽ALOHA算法来解决多标签识别时产生的碰撞问题。文章首先介绍了EPC-C1G2协议多标签碰撞解决所需的参数和命令,指出协议中的Q值决定了解决碰撞时所用的时槽数。时槽数越多,标签越不容易碰撞,但识别时间越长,因此,恰当地选择Q值可以在标签识别时间和识别标签数之间找到最佳点。文中同时给出了一种动态Q值算法,该算法可根据标签碰撞解决的实际情况动态调整Q值,使标签识别更高效。文章最后对这种多标签识别流程的性能进行了仿真分析。     

Secure authentication scheme for passive C1G2 RFID tags

Privacy and security concerns inhibit the fast adaption of RFID technology for many applications. A number of authentication protocols that address these concerns have been proposed but real-world solutions that are secure, maintain low communication cost and can be integrated into the ubiquitous EPCglobal Class 1 Generation 2 tag protocol (C1G2) are still needed and being investigated. We present a novel authentication protocol, which offers a high level of security through the combination of a random key scheme with a strong cryptography. The protocol is applicable to resource, power and computationally constraint platforms such as RFID tags. Our investigation shows that it can provide mutual authentication, untraceability, forward and backward security as well as resistance to replay, denial-ofth-service and man-in-the-middle attacks, while retaining a competitive communication cost. The protocol has been integrated into the EPCglobal C1G2 tag protocol, which assures low implementation cost. We also present a successful implementation of our protocol on real-world components such as the INTEL WISP UHF RFID tag and a C1G2 compliant reader.     

基于反向散射和地面标识的无源RFID标签的位置检测方法

该文提出了一种针对无源RFID标签的位置检测的方法。此方法将反向散射调制与地面标识相结合来估计标签的当前位置,不需要改变当前的RFID标准,利用现有的硬件条件就可以实现。文章首先介绍反向散射调制和地面标示估算标签位置的方法,并提出两种方法混合计算的优化算法流程,另外方法的改进措施也被提了出来。最后通过实验和仿真来说明这种方法的有效性和稳定性。     

RFID技术在智能交通管理中应用

本文主要介绍RFDI技术定义及系统工作原理,并重点关注RFID技术在智能交通领域的实际应用方式。同时RFID技术也成功解决了车辆自动识别和信息采集问题,为智能交通管理和服务系统的建立提供了强大的信息支撑和保障。     

Orientated Immobilization of FAD-Dependent Glucose Dehydrogenase on Electrode by Carbohydrate-Binding Module Fusion for Efficient Glucose Assay

The discovery or engineering of fungus-derived FAD-dependent glucose 1-dehydrogenase (FAD-GDH) is especially important in the fabrication and performance of glucose biosensors. In this study, a novel FAD-GDH gene, phylogenetically distantly with other FAD-GDHs from Aspergillus species, was identified. Additionally, the wild-type GDH enzyme, and its fusion enzyme (GDH-NL-CBM2) with a carbohydrate binding module family 2 (CBM2) tag attached by a natural linker (NL), were successfully heterogeneously expressed. In addition, while the GDH was randomly immobilized on the electrode by conventional methods, the GDH-NL-CBM2 was orientationally immobilized on the nanocellulose-modified electrode by the CBM2 affinity adsorption tag through a simple one-step approach. A comparison of the performance of the two electrodes demonstrated that both electrodes responded linearly to glucose in the range of 0.12 to 40.7 mM with a coefficient of determination R² > 0.999, but the sensitivity of immobilized GDH-NL-CBM2 (2.1362 × 10⁻² A/(M*cm²)) was about 1-fold higher than that of GDH (1.2067 × 10⁻² A/(M*cm²)). Moreover, a lower detection limit (51 µM), better reproducibility (<5%) and stability, and shorter response time (≈18 s) and activation time were observed for the GDH-NL-CBM2-modified electrode. This facile and easy immobilization approach used in the preparation of a GDH biosensor may open up new avenues in the development of high-performance amperometric biosensors.     

基于RFID的虚拟标签算法研究与改进

针对室内定位算法VIRE的定位精度,特别是邻近边界目标的定位精度较低的问题,以RFID标签定位为研究基础,从改变VIRE算法中虚拟标签的插值方式和在边界上加入虚拟标签两个方面进行研究和改进。使用拉格朗日插值代替VIRE算法中的线性插值方式,使虚拟标签的数据更加接近实际环境值;在边界上加入虚拟参考标签能增加已知标签数,防止在计算待定位标签坐标时引入误差标签。实验结果表明,标签定位精度有了明显提高,整体能提高了35%,邻近边界标签定位精度提高较大,可达到50%以上。     

一种基于同态标签的动态云存储数据完整性验证方法

在云存储服务中,为使用户可以随时验证存储在云存储服务器上数据的完整性,提出一种基于同态标签的动态数据完整性验证方法。通过引入同态标签和用户随机选择待检测数据块,可以无限次验证数据是否完好无损,并支持数据动态更新;可信第三方的引入解决了云用户与云存储服务供应商因数据完整性问题产生的纠纷,实现数据完整性的公开验证;然后给出该方法的正确性和安全性分析,以及该方法的性能分析;最后通过实验验证了该方法是高效可行的。     

基于DCT和感知哈希的纹理防伪标签鉴别算法

针对纹理防伪标签在光线不足、视力不佳的情况下难以鉴别的问题,提出了一种基于DCT、感知哈希和视觉特征向量的纹理防伪标签鉴别算法.首先通过DCT和感知哈希提取图像的特征向量;然后建立特征数据库,通过特征向量之间的归一化相关系数(NC)实现纹理防伪标签的自动鉴别.实验结果表明,该方法可以有效抵抗常规攻击和几何攻击,可实现自动鉴别技术.与现有的纹理防伪技术相比,该算法可以减小占据的数据库存储空间,具有更好的实用性.