Multiplex PCR system to track authorized and unauthorized genetically modified soybean events in food and feed

A diverse range of genetic elements has been used to develop genetically modified organisms (GMOs) over the last 18 years. Screening methods that target few elements, such as the Cauliflower Mosaic Virus 35S promoter (P-35S) and Agrobacterium tumefaciens nopaline terminator (T-nos), are not sufficient to screen GMOs. In the present study, a multiplex PCR system for all globally commercialized GM soybean events was developed to easily trace the events. For this purpose, screening elements of 24 GM soybean events were investigated and 9 screening targets were selected and divided into three individual triplex PCR systems: P-35S, ribulose-1,5-bisphosphate carboxylase small subunit promoter of Arabidopsis thaliana, T-nos, T-35S, pea E9 terminator, open reading frame 23 terminator of A. tumefaciens, proteinase inhibitor II terminator of potato, acetohydroxy acid synthase large subunit terminator of A. thaliana, and the revealed 3′ flanking sequences of DP-305423-1. The specificity of the assays was confirmed using thirteen GM soybean events as the respective positive/negative controls. The limit of detection of each multiplex set, as determined using certified reference materials of specific GM events, ranged from 0.03 to 0.5%, depending upon target. Furthermore, 26 food samples that contained soybean ingredients, which were purchased from the USA, China, Japan, and Korea, were analyzed, 17 of which contained one or more GM soybean events. These results suggest that the developed screening method can be used to efficiently track and identify 24 GM soybean events in food and feed.     

一种调整簇阀值的加速聚类分析算法及其应用

针对航运信息中大样本聚类问题,根据k-means聚类过程中大部分簇的调整发生在初始迭代阶段的特性,提出了一种调整簇阀值的加速聚类方法,并对该算法进行实例测试,实验结果证明了该方法在满足原有的聚类精度的基础上,减少了聚类的计算量。本文将该方法应用到船舶航线设计中。     

应用改进频率调谐的海上小目标检测方法

提出了一种新的基于可见光图像的海上小目标检测方法。由于频率调谐方法将图像空间域整体均值与高斯滤波后差分结果,作为显著性度量的标准,因此当图像背景中存在较多杂波干扰时,显著目标检测效果不理想。提出的方法对基于频率调谐的显著性检测方法进行了改进,首先对图像LAB空间中3个特征分量进行分块,在每个分块区域中应用频率调谐显著性检测方法,进而将其结果合并为总显著图,以检测海上小目标。该方法克服了频率调谐方法,当海面背景中存在大量海杂波,无法有效提取小目标的缺陷。实验结果表明了该方法的有效性。     

Rescheduling policies for large-scale task allocation of autonomous straddle carriers under uncertainty at automated container terminals

This paper investigates replanning strategies for container-transportation task allocation of autonomous Straddle Carriers (SC) at automated container terminals. The strategies address the problem of large-scale scheduling in the context of uncertainty (especially uncertainty associated with unexpected events such as the arrival of a new task). Two rescheduling policies–Rescheduling New arrival Jobs (RNJ) policy and Rescheduling Combination of new and unexecuted Jobs (RCJ) policy–are presented and compared for long-term Autonomous SC Scheduling (ASCS) under the uncertainty of new job arrival. The long-term performance of the two rescheduling policies is evaluated using a multi-objective cost function (i.e., the sum of the costs of SC travelling, SC waiting, and delay of finishing high-priority jobs). This evaluation is conducted based on two different ASCS solving algorithms–an exact algorithm (i.e., branch-and-bound with column generation (BBCG) algorithm) and an approximate algorithm (i.e., auction algorithm)–to get the schedule of each short-term planning for the policy. Based on the map of an actual fully-automated container terminal, simulation and comparative results demonstrate the quality advantage of the RCJ policy compared with the RNJ policy for task allocation of autonomous straddle carriers under uncertainty. Long-term testing results also show that although the auction algorithm is much more efficient than the BBCG algorithm for practical applications, it is not effective enough, even when employed by the superior RCJ policy, to achieve high-quality scheduling of autonomous SCs at the container terminals.     

城市交通网络连通可靠度计算研究

考虑路段、交叉口运营状态的随机变化,以饱和度作为交通网络连通可靠度的主要影响因素,完善路段连通可靠度的计算式,给出了交叉口可靠度的衡量指标;结合路段、交叉口可靠度特别是交叉口不同转向的可靠度,设计了一种生成树算法寻找交通网络的不交型通路,进而快速计算交通网络中任一OD对间的连通可靠度。算例表明,交叉口的可靠度对城市连通可靠度有重要影响,提出的算法具有一定的应用价值和借鉴意义。     

2009年度中国股市IPO抑价实证研究

在阐述我国IPO现状及回顾国内外经典册抑价理论的基础上,本文对所选取样本新股的高抑价率影响因素进行了多元回归实证分析,通过分析找出了新股抑价率与影响因素之间的相关关系,最后在结论的基础上提出了政策建议．     

基于流水线装配生产率问题的Spreadsheet仿真模型

在流水线生产中,由于两工序间存储空间有限,前序工人与后续工人之间相互影响.为了研究流水线中存储空间对生产率的影响,提高流水线工人生产率,本文利用Spreadsheet建立仿真模型,通过仿真模拟,研究分析流水线生产中两工序之间没有存储空间时及增加存储空间后对生产效率的影响.为解决流水线装配问题提供一种新的方法.     

用高分子材料修复舰艇大轴

舰艇大轴在海水中严重腐蚀后,采用粘接技术,选用国产高强度金属修补剂,对大轴局部腐蚀部位进行修理,对提高大轴使用寿命,防止大轴再腐蚀起到有效作用.     

高强度低合金铌钒钢板的失效分析

针对高强度低合金铌钒钢板在拉伸试验后出现分层开裂的现象,采用SEM、EDAX进行微观分析,发现试样内部有大量夹杂物存在,这些夹杂物主要含有钙、氧、硫、锰等元素。带有缺陷的钢板若应用到实际工程中,会影响铁塔的寿命。     

改进的遗传算法应用于碎片拼接

碎片拼接实际用途已经越来越广泛,本文讲述传统碎片拼接的方法,引出图形匹配策略,传统的碎片拼接算法大多需要大量繁琐的计算,大大增加了算法的时间复杂度,依据传统算法的不足,改进遗传算法应用于碎片匹配策略,提出可以实现更高效率、更高准确率的碎片拼接算法。