Multiplex PCR system to track authorized and unauthorized genetically modified soybean events in food and feed

A diverse range of genetic elements has been used to develop genetically modified organisms (GMOs) over the last 18 years. Screening methods that target few elements, such as the Cauliflower Mosaic Virus 35S promoter (P-35S) and Agrobacterium tumefaciens nopaline terminator (T-nos), are not sufficient to screen GMOs. In the present study, a multiplex PCR system for all globally commercialized GM soybean events was developed to easily trace the events. For this purpose, screening elements of 24 GM soybean events were investigated and 9 screening targets were selected and divided into three individual triplex PCR systems: P-35S, ribulose-1,5-bisphosphate carboxylase small subunit promoter of Arabidopsis thaliana, T-nos, T-35S, pea E9 terminator, open reading frame 23 terminator of A. tumefaciens, proteinase inhibitor II terminator of potato, acetohydroxy acid synthase large subunit terminator of A. thaliana, and the revealed 3′ flanking sequences of DP-305423-1. The specificity of the assays was confirmed using thirteen GM soybean events as the respective positive/negative controls. The limit of detection of each multiplex set, as determined using certified reference materials of specific GM events, ranged from 0.03 to 0.5%, depending upon target. Furthermore, 26 food samples that contained soybean ingredients, which were purchased from the USA, China, Japan, and Korea, were analyzed, 17 of which contained one or more GM soybean events. These results suggest that the developed screening method can be used to efficiently track and identify 24 GM soybean events in food and feed.     

一种调整簇阀值的加速聚类分析算法及其应用

针对航运信息中大样本聚类问题,根据k-means聚类过程中大部分簇的调整发生在初始迭代阶段的特性,提出了一种调整簇阀值的加速聚类方法,并对该算法进行实例测试,实验结果证明了该方法在满足原有的聚类精度的基础上,减少了聚类的计算量。本文将该方法应用到船舶航线设计中。     

Rescheduling policies for large-scale task allocation of autonomous straddle carriers under uncertainty at automated container terminals

This paper investigates replanning strategies for container-transportation task allocation of autonomous Straddle Carriers (SC) at automated container terminals. The strategies address the problem of large-scale scheduling in the context of uncertainty (especially uncertainty associated with unexpected events such as the arrival of a new task). Two rescheduling policies–Rescheduling New arrival Jobs (RNJ) policy and Rescheduling Combination of new and unexecuted Jobs (RCJ) policy–are presented and compared for long-term Autonomous SC Scheduling (ASCS) under the uncertainty of new job arrival. The long-term performance of the two rescheduling policies is evaluated using a multi-objective cost function (i.e., the sum of the costs of SC travelling, SC waiting, and delay of finishing high-priority jobs). This evaluation is conducted based on two different ASCS solving algorithms–an exact algorithm (i.e., branch-and-bound with column generation (BBCG) algorithm) and an approximate algorithm (i.e., auction algorithm)–to get the schedule of each short-term planning for the policy. Based on the map of an actual fully-automated container terminal, simulation and comparative results demonstrate the quality advantage of the RCJ policy compared with the RNJ policy for task allocation of autonomous straddle carriers under uncertainty. Long-term testing results also show that although the auction algorithm is much more efficient than the BBCG algorithm for practical applications, it is not effective enough, even when employed by the superior RCJ policy, to achieve high-quality scheduling of autonomous SCs at the container terminals.     

基于流水线装配生产率问题的Spreadsheet仿真模型

在流水线生产中,由于两工序间存储空间有限,前序工人与后续工人之间相互影响.为了研究流水线中存储空间对生产率的影响,提高流水线工人生产率,本文利用Spreadsheet建立仿真模型,通过仿真模拟,研究分析流水线生产中两工序之间没有存储空间时及增加存储空间后对生产效率的影响.为解决流水线装配问题提供一种新的方法.     

用高分子材料修复舰艇大轴

舰艇大轴在海水中严重腐蚀后,采用粘接技术,选用国产高强度金属修补剂,对大轴局部腐蚀部位进行修理,对提高大轴使用寿命,防止大轴再腐蚀起到有效作用.     

高强度低合金铌钒钢板的失效分析

针对高强度低合金铌钒钢板在拉伸试验后出现分层开裂的现象,采用SEM、EDAX进行微观分析,发现试样内部有大量夹杂物存在,这些夹杂物主要含有钙、氧、硫、锰等元素。带有缺陷的钢板若应用到实际工程中,会影响铁塔的寿命。     

改进的遗传算法应用于碎片拼接

碎片拼接实际用途已经越来越广泛,本文讲述传统碎片拼接的方法,引出图形匹配策略,传统的碎片拼接算法大多需要大量繁琐的计算,大大增加了算法的时间复杂度,依据传统算法的不足,改进遗传算法应用于碎片匹配策略,提出可以实现更高效率、更高准确率的碎片拼接算法。     

集装箱码头三维仿真组件平台

为直观、形象地研究集装箱码头生产优化问题,采用三维仿真的方法实现集装箱码头物流信息与作业过程的可视化．基于不同业务流程的仿真要求,合理地划分静态布局、装卸设备、装卸对象、码头操作、运动学解算、数据访问、通信接口和图形渲染等组件的粒度;建立层次结构形式的组件平台,体现客观对象的逻辑关系;遵循标准COM＋规范设计岸桥、场桥和集卡等集装箱码头主要三维仿真组件．以上海振华重工（集团）股份有限公司自动化集装箱码头为例验证该组件平台的可操作性,结果表明该方法对集装箱码头装卸工艺的改进和管理水平的提升具有实际指导意义．     

基于NPSO算法求解车间作业调度问题

研究车间生产生产调试系统,使资源达到优化配置,实现了一种基于小生境的粒子群优化算法用于求解车间作业调度问题。通过在粒子群算法中引入共享函数和共享适应度函数分别用来计算粒子间的共享度和粒子的共享适应值,并用粒子的共享适应值来反映其适应能力。粒子的位置越相近,则粒子间的共享度越大,相应粒子的共享适应值则越小。通过设置小生境半径的方式,将整个粒子群分解为多个小生境子种群,并通过设置小生境中的最大粒子个数参数,严格控制各个小生境中的粒子数量,使得所有粒子尽可能地分布到整个搜索空间的不同局部峰值区域,从而有效求得问题的全局最优值。仿真结果表明了算法对经典JSP问题求解的优良性能。     

JSP问题的遗传算法解决途径

针对车床作业调度问题,讨论了应用于车床作业调度的遗传算法设计,给出了主要的编码、解码、以及死锁问题的算法模型。结合应用实例．说明了设计的可行性与有效性。