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1.
随着人们日益增长的生活水平,我国电力管理体制改革也在逐步的深化,人们对于电力资源的需求不断提升,电力基建工程建设与供电的质量有待进一步加强。电力施工企业应充分认识到电力工程项目施工过程中安全管理的重要性,明确电力工程项目施工安全给企业带来的安全效益。本文对电力工程基建施工现场所存在的一些实际问题进行分析和研究,并总结施工现场“7s管理”模式的应用前景以及管理方面的要求,希望能够为我国的电力工程基础建设有所帮助。  相似文献   
2.
Spinal muscular atrophy (SMA) is an autosomal recessive hereditary neuromuscular disease. Exon 7 and 8 of survival of motor neuron 1 (SMN1) gene or only exon 7 homology deletion leads to the failure to produce a full-length SMN gene. The copy number of SMN2 gene with high homology of SMN1 affects the degree of disease and was the target gene for targeting therapy, in which splicing silencer in intron 7 was the key to suppress the inclusion of exon 7. In this study, we projected to use CRISPR/Case 9 for the targeted editing of intronic-splicing silencer (ISS) sequence to promote the inclusion of SMN2 exon 7 and increase the production of SMN2 full-length (FL) gene expression. It happens that there was a protospacer adjacent motif (PAM) at one end of the ISS sequence according to the design of sgRNA. The recombinant vector of sgRNA HSMN2 CRISPR/Case 9 was constructed and transfected into HEK293 cells. Sequencing results showed that the ISS sequence could be edited accurately and targeting in the predicted direction, in which deleting small fragments, inserting small amounts and mutation. Quantitative analysis of RT-PCR products by restriction enzyme of DdeI digestion showed that the FL of SMN2 increased by 8% (P < 0.05). In the primary cultured chondrocytes of SMA mice, in which sgRNA HSMN2 CRISPR/Case9 recombinant vector transfection could increase the SMN2 FL gene by 23% (P < 0.05) and significantly improve SMN protein levels (P < 0.05). CRISPR/Case 9 is an effective tool for gene editing and therapy of hereditary diseases, but it is rarely reported in the treatment of SMA diseases. This study shows that CRISPR/Case 9 was first used for the precision target of ISS sequence editing, which can effectively promote the production of SMN2 FL gene expressions, in which there was an important clinical reference value.  相似文献   
3.
大尺寸弹丸喷丸成形是一种针对大型整体壁板塑性成形的有效手段。采用正交试验方法对7B50铝合金平板试件开展大尺寸弹丸喷丸成形工艺试验,研究不同工艺参数下喷丸后试件沿弦向和展向曲率半径的变化规律。结果表明:试件在喷丸成形后呈球面凸起变形,且沿弦向的曲率半径小于沿展向的曲率半径;各因素按对曲率半径的影响程度排序依次为喷丸气压>试件厚度>移动速度;曲率半径随着厚度和移动速度的增加而增加,随着喷丸气压的增加而减小;建立了可以反映曲率半径与3个因素之间定量关系的回归方程,可为今后喷丸成形的变形预测提供参考。  相似文献   
4.
Phosphodiesterase 7 (PDE7) is an enzyme responsible for the degradation of cyclic adenosine monophosphate (cAMP), an important cellular messenger. PDE7’s role in neurotransmission, expression profile in the brain and the druggability of other phosphodiesterases have motivated the search for potent inhibitors to treat neurodegenerative and inflammatory diseases. Different heterocyclic compounds have been described over the years; among them, phenyl-2-thioxo-(1H)-quinazolin-4-one, called S14, has shown very promising results in different in vitro and in vivo studies. Recently, polymeric nanoparticles have been used as new formulations to target specific organs and produce controlled release of certain drugs. In this work, we describe poly(lactic-co-glycolic acid) (PLGA)-based polymeric nanoparticles loaded with S14. Their preparation, optimization, characterization and in vivo drug release profile are here presented as an effort to improve pharmacokinetic properties of this interesting PDE7 inhibitor.  相似文献   
5.
TRPM7 plays an important role in cellular Ca2+, Zn2+ and Mg2+ homeostasis. TRPM7 channels are abundantly expressed in ameloblasts and, in the absence of TRPM7, dental enamel is hypomineralized. The potential role of TRPM7 channels in Ca2+ transport during amelogenesis was investigated in the HAT-7 rat ameloblast cell line. The cells showed strong TRPM7 mRNA and protein expression. Characteristic TRPM7 transmembrane currents were observed, which increased in the absence of intracellular Mg2+ ([Mg2+]i), were reduced by elevated [Mg2+]i, and were inhibited by the TRPM7 inhibitors NS8593 and FTY720. Mibefradil evoked similar currents, which were suppressed by elevated [Mg2+]i, reducing extracellular pH stimulated transmembrane currents, which were inhibited by FTY720. Naltriben and mibefradil both evoked Ca2+ influx, which was further enhanced by the acidic intracellular conditions. The SOCE inhibitor BTP2 blocked Ca2+ entry induced by naltriben but not by mibefradil. Thus, in HAT-7 cells, TRPM7 may serves both as a potential modulator of Orai-dependent Ca2+ uptake and as an independent Ca2+ entry pathway sensitive to pH. Therefore, TRPM7 may contribute directly to transepithelial Ca2+ transport in amelogenesis.  相似文献   
6.
ER7车轮钢经不同工艺热处理后,可获得珠光体片层间距以及铁素体含量不同的显微组织,并对不同工艺处理试样的拉伸性能及-20 ℃冲击性能进行了测试。结果表明,随冷却速度的增大,车轮钢铁素体含量增加,珠光体片间距和珠光体球团尺寸减小。增大冷却速率,会使车轮钢的屈服强度、抗拉强度、伸长率和断面收缩率都随之增加。随着珠光体片间距和晶粒尺寸减小,车轮钢的断裂韧性也相应增大。在850 ℃加热并通过水雾冷却后的车轮钢试样强韧匹配最好,综合力学性能最好。  相似文献   
7.
The recessive form of dystrophic epidermolysis bullosa (RDEB) is a crippling disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Using ectopic expression of hTERT/hTERT + BMI-1 in primary cells, we developed expansible cultures of RDEB fibroblasts and keratinocytes. We showed that they display the properties of their founders, including morphology, contraction ability and expression of the respective specific markers including reduced secretion of type VII collagen (C7). The immortalized keratinocytes retained normal stratification in 3D skin equivalents. The comparison of secreted protein patterns from immortalized RDEB and healthy keratinocytes revealed the differences in the contents of the extracellular matrix that were earlier observed specifically for RDEB. We demonstrated the possibility to reverse the genotype of immortalized cells to the state closer to the progenitors by the Cre-dependent hTERT switch off. Increased β-galactosidase activity and reduced proliferation of fibroblasts were shown after splitting out of transgenes. We anticipate our cell lines to be tractable models for studying RDEB from the level of single-cell changes to the evaluation of 3D skin equivalents. Our approach permits the creation of standardized and expandable models of RDEB that can be compared with the models based on primary cell cultures.  相似文献   
8.
利用万能试验机和Hopkinson拉杆(SHTB)对7A62铝合金进行了准静态和动态拉伸性能测试,研究该合金室温及高温塑性流动应力动态响应特征,结合OM、SEM、TEM、DSC、LFA等测试对该合金的物理性能及原始微观组织进行分析。结果表明:7A62铝合金在大量高密度细小沉淀析出物粒子及亚微米级高熔点平衡析出粒子复合强化作用下,准静态屈服强度可达608 MPa。在室温动态变形过程中,该合金应变率强化效应显著。随着应变率高于684 s^-1时,合金屈服强度对应变率敏感性显著增强。在1100 s^-1、25~500℃条件下,合金表现出温度敏感性的沉淀强化相回溶及动态再结晶的热软化效应,500℃高温动态屈服强度超过200 MPa。在动态力学性能变化规律的基础上,建立了7A62铝合金的Johnson-Cook(J-C)本构模型。  相似文献   
9.
针对高强铝合金航空复杂构件室温成形难问题,通过电阻炉加热及冲压成形试验探究了温热条件对7B04铝合金硬度及成形性能的影响规律,通过差示扫描量热仪及透射电镜探讨其性能变化机理。研究结果表明:7B04铝合金加热后,升温速率越快,材料硬度下降越快;在400 ℃以上炉温条件下加热较长时间,硬度会先降后升;在200~450 ℃温度范围内,7B04板材冲压成形性能随出炉温度提高而提高,加热至400 ℃以上出炉冲压成形效果较好,升温速率的提高可以使7B04铝合金在硬度降幅相同的情况下,达到更高的成形温度,获得更好的成形性能。  相似文献   
10.
鄂尔多斯盆地三叠系延长组7段(长7段)泥页岩发育,针对其内部富集的页岩型页岩油尚未取得勘探进展,仅在少量直井的试油阶段突破出油关,但试采效果整体较差。系统梳理了长7段3亚段(长73亚段)泥页岩的岩石学、地球化学和储层特征,优选页岩型页岩油的富集有利参数,明确了其成藏条件、勘探方向及潜力。相较于暗色泥岩,长73亚段的黑色页岩表现为多类型纹层频繁发育、有机质丰度高、生油母质类型好、主生烃期早、含油率高的特征,是页岩型页岩油勘探的有利岩性。受水平页理缝和陆源长英质、凝灰质等相对高孔高渗纹层的复合影响,黑色页岩的水平渗透率普遍高于垂直渗透率。长73亚段泥页岩的储集空间以刚性矿物支撑的粒间孔、粒内溶蚀孔、黏土矿物晶间孔及黄铁矿晶间孔为主,页理缝和超压微裂缝较发育。"有机质+陆源长英质纹层"、"有机质+凝灰质纹层"二元纹层组合与页理缝构成的复合单元对页岩油赋存最为有利。总有机碳(TOC)含量为4%~14%、镜质体反射率Ro>0.8%、页理及纹层发育、脆性矿物含量高的长英质黑色页岩是长73亚段页岩型页岩油的有利勘探目标,黑色页岩厚度相对较大、有机质丰度适中的马岭—华池深洼区是页岩型页岩油勘探的Ⅰ类有利区。鄂尔多斯盆地长73亚段页岩型页岩油资源丰富,有望成为盆地非常规石油勘探开发的现实性重大战略接替新领域。  相似文献   
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