首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   158199篇
  免费   21033篇
  国内免费   12446篇
电工技术   11756篇
技术理论   14篇
综合类   15277篇
化学工业   13016篇
金属工艺   5172篇
机械仪表   8204篇
建筑科学   7562篇
矿业工程   3909篇
能源动力   1910篇
轻工业   11357篇
水利工程   2704篇
石油天然气   3160篇
武器工业   2176篇
无线电   27505篇
一般工业技术   14167篇
冶金工业   5401篇
原子能技术   1258篇
自动化技术   57130篇
  2024年   246篇
  2023年   3032篇
  2022年   5525篇
  2021年   7120篇
  2020年   6008篇
  2019年   5026篇
  2018年   4774篇
  2017年   5280篇
  2016年   6360篇
  2015年   7822篇
  2014年   11548篇
  2013年   11125篇
  2012年   12056篇
  2011年   11797篇
  2010年   9071篇
  2009年   9432篇
  2008年   9759篇
  2007年   11607篇
  2006年   10520篇
  2005年   9136篇
  2004年   6967篇
  2003年   6080篇
  2002年   4337篇
  2001年   3213篇
  2000年   2520篇
  1999年   1932篇
  1998年   1410篇
  1997年   1161篇
  1996年   1103篇
  1995年   919篇
  1994年   790篇
  1993年   611篇
  1992年   505篇
  1991年   414篇
  1990年   376篇
  1989年   276篇
  1988年   227篇
  1987年   157篇
  1986年   144篇
  1985年   146篇
  1984年   162篇
  1983年   120篇
  1982年   95篇
  1981年   85篇
  1980年   87篇
  1979年   59篇
  1978年   37篇
  1977年   37篇
  1964年   46篇
  1962年   68篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Deposition of amyloid β (Aβ) fibrils in the brain is a key pathologic hallmark of Alzheimer’s disease. A class of polyphenolic biflavonoids is known to have anti-amyloidogenic effects by inhibiting aggregation of Aβ and promoting disaggregation of Aβ fibrils. In the present study, we further sought to investigate the structural basis of the Aβ disaggregating activity of biflavonoids and their interactions at the atomic level. A thioflavin T (ThT) fluorescence assay revealed that amentoflavone-type biflavonoids promote disaggregation of Aβ fibrils with varying potency due to specific structural differences. The computational analysis herein provides the first atomistic details for the mechanism of Aβ disaggregation by biflavonoids. Molecular docking analysis showed that biflavonoids preferentially bind to the aromatic-rich, partially ordered N-termini of Aβ fibril via the π–π interactions. Moreover, docking scores correlate well with the ThT EC50 values. Molecular dynamic simulations revealed that biflavonoids decrease the content of β-sheet in Aβ fibril in a structure-dependent manner. Hydrogen bond analysis further supported that the substitution of hydroxyl groups capable of hydrogen bond formation at two positions on the biflavonoid scaffold leads to significantly disaggregation of Aβ fibrils. Taken together, our data indicate that biflavonoids promote disaggregation of Aβ fibrils due to their ability to disrupt the fibril structure, suggesting biflavonoids as a lead class of compounds to develop a therapeutic agent for Alzheimer’s disease.  相似文献   
2.
This study evaluated the direct effect of a phytochemical, hesperidin, on pre-osteoblast cell function as well as osteogenesis and collagen matrix quality, as there is little known about hesperidin’s influence in mineralized tissue formation and regeneration. Hesperidin was added to a culture of MC3T3-E1 cells at various concentrations. Cell proliferation, viability, osteogenic gene expression and deposited collagen matrix analyses were performed. Treatment with hesperidin showed significant upregulation of osteogenic markers, particularly with lower doses. Mature and compact collagen fibrils in hesperidin-treated cultures were observed by picrosirius red staining (PSR), although a thinner matrix layer was present for the higher dose of hesperidin compared to osteogenic media alone. Fourier-transform infrared spectroscopy indicated a better mineral-to-matrix ratio and matrix distribution in cultures exposed to hesperidin and confirmed less collagen deposited with the 100-µM dose of hesperidin. In vivo, hesperidin combined with a suboptimal dose of bone morphogenetic protein 2 (BMP2) (dose unable to promote healing of a rat mandible critical-sized bone defect) in a collagenous scaffold promoted a well-controlled (not ectopic) pattern of bone formation as compared to a large dose of BMP2 (previously defined as optimal in healing the critical-sized defect, although of ectopic nature). PSR staining of newly formed bone demonstrated that hesperidin can promote maturation of bone organic matrix. Our findings show, for the first time, that hesperidin has a modulatory role in mineralized tissue formation via not only osteoblast cell differentiation but also matrix organization and matrix-to-mineral ratio and could be a potential adjunct in regenerative bone therapies.  相似文献   
3.
4.
针对移动机器人使用超声波传感器检测环境时存在干扰与数据不确定性问题,在分析超声波传感器工作原理和相邻位置检测数据的关联特性后,提出了基于三位置超声波检测的环境轮廓构建方法,利用超声波对室内环境进行建图;再使用改进强跟踪UKF-SLAM将超声波测量数据和移动机器人驱动模型进行滤波融合,得到更准确的位姿信息与地图特征。搭建仿真环境,并通过搭载有超声波传感器的全向轮移动机器人在实验环境内验证。仿真结果表明改进方法与其他算法相比,定位和地图构建的误差降低58.058%。室内实验中,获取环境特征的平均误差降低了50.286 3%,进一步验证了提出算法的可行性与有效性。该方法对机器人同步定位与地图构建有一定参考价值。  相似文献   
5.
磁感应断层成像作为一种新的检测成像技术,在脑出血等疾病的临床诊断和连续检测中具有重要的应用价值。 磁感应 断层成像系统由激励线圈和检测线圈两种线圈组成,其中激励线圈只有一个,而检测线圈在以往的研究中多达十几个甚至几十 个。 由于空间限制以及线圈之间的相互干扰,如何在不损失成像准确度的情况下最大程度减少线圈的个数具有重要的研究意 义。 针对此问题,本文提出了仅使用 5 个检测线圈,分别检测两个相互垂直方向磁场信息的双向检测成像方法。 进行了含有半 径为 15 mm 的单个异质体和半径分别为 7 和 15 mm 的两个异质体的复杂头部模型仿真实验和异质体检测的仿体实验,结果均 表明双向检测成像方式不仅可以抵消检测线圈个数减少带来的影响,而且具有更高的抗噪性能和成像准确度,从而为推动 MIT 的实际应用奠定了技术基础。  相似文献   
6.
为研究攀爬机器人在港口机械金属结构表面的自主移动,提出了一种应用于攀爬机器人的视觉导航定位方法。针对港口机械金属结构复杂,表面存在油污和阴影,解决现有的视觉边缘识别方法对金属结构边缘识别效率低且不连续的问题。首先通过自适应阈值的Canny进行边缘特征提取;之后根据边缘线角度特征建立约束关系,利用Hough变换剔除干扰线,并用最小二乘法拟合最终金属结构边缘;求取结构中心线方程和运动方程并计算偏航角和偏距,实现对机器人的运动控制。以港口机械某一支腿为例进行试验,实验数据表明,该方法可以很好地引导机器人的运动,满足实际应用。  相似文献   
7.
在电力信息化战略的不断深入下,电力巡检机器人日益普及,为完善机器人功能,拓宽其应用前景,提出一种挂空异物智能检测方法。根据光照强度、天气状况等因素,基于不同颜色空间变量值与背景特征关联,合理利用对应滤波方式去除图像内噪声杂质。面向模糊不清的采集图像,依据前景与背景的分割阈值和平均灰度值,改变分段区间与拉伸区间,增强图像对比度。结合输电线路的直线特征与点线对偶特性,以霍夫变换直线提取法为基础识别出输电线路;通过设计的拟合恢复方法,获取完整的输电线路由局部二值模式图像特征点匹配策略,建立矩形兴趣范围,遍历像素点,检测出线路上的挂空异物。实验环节分别通过检测图像与评估指标数据,从主客观角度全面分析了检测效果,经实验结果验证,所提方法具有良好的可行性与适用性,能够较为准确地检测出多种异物。  相似文献   
8.
This paper focuses on the configuration design of flexure hinges with a prescribed compliance matrix and preset rotational center position. A new method for the topology optimization of flexure hinges is proposed based on the adaptive spring model and stress constraint. The hinge optimization model is formulated by maximizing the bending displacement with a spring while optimizing the compliance matrix to a prescribed value. To avoid numerical instability, an artificial spring is used as an auxiliary calculation, and a new strategy is developed for adaptively adjusting the spring stiffness according to the prescribed compliance matrix. The maximum stress of flexure hinge is limited by using a normalized P-norm of the effective von Mises stress, and a position constraint of rotational center is proposed to predetermine the position of the rotational center. In addition, to reduce the error of the stress measurement, a simple but effective filtering method is presented to obtain a complete black-and-white design. Numerical examples are used to verify the proposed method. Topology results show that the obtained flexure hinges have the prescribed compliance matrix and preset rotational center position while also meeting the stress requirements.  相似文献   
9.
The Gli-B1-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1-γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B. It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i, k, m, o), II (Gli-B1n, q, s), III (Gli-B1b), IV (Gli-B1e, f, g), V (Gli-B1h), VI (Gli-B1d) and VII (Gli-B1a). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat.  相似文献   
10.
In this study, we investigate the immunomodulatory effects of a novel antimicrobial peptide, YD1, isolated from Kimchi, in both in vitro and in vivo models. We establish that YD1 exerts its anti-inflammatory effects via up-regulation of the Nrf2 pathway, resulting in the production of HO-1, which suppresses activation of the NF-κB pathway, including the subsequent proinflammatory cytokines IL-1β, IL-6, and TNF-α. We also found that YD1 robustly suppresses nitric oxide (NO) and prostaglandin E2 (PGE2) production by down-regulating the expression of the upstream genes, iNOS and COX-2, acting as a strong antioxidant. Collectively, YD1 exhibits vigorous anti-inflammatory and antioxidant activity, presenting it as an interesting potential therapeutic agent.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号