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Akihiro Hara Mitsuyoshi Ueda Toru Matsui Keizo Furuhashi Naoki Kanayama Atsuo Tanaka 《Journal of Bioscience and Bioengineering》1999,87(6):717
A transformation system using the autonomously replicating plasmid in the n-alkane-assimilating and asporogenic diploid yeast, Candida tropicalis, was developed. For the cloning of a DNA fragment containing a potential autonomously replicating sequence (ARS) from the genomic DNA of C. tropicalis, the ura3 mutant obtained using ethylmethane sulfonate as the host and the URA3 gene amplified by PCR using the C. tropicalis genomic DNA as a selectable marker were prepared. Comparison of ARSs among yeasts revealed that the consensus sequence found in S. cerevisiae was also present in C. tropicalis. The autonomously replicating plasmid containing the putative ARS as the shuttle vector, capable of replicating in both E. coli and C. tropicalis, was first constructed. The transformation system using this plasmid, in addition to the integrative transformation system, will be applicable to genetic studies of C. tropicalis. 相似文献
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地下铲运机依靠车载控制器输出的指令控制行驶方向,实现自主行驶。虽然控制器输出是线性的,但是地下铲运机动作却不是,当输出值过小,地下铲运机不转向,存在转向死区现象。对此开展研究,通过对死区的理论分析,试验测试,优化地下铲运机的控制算法,进而减小地下铲运机在行驶过程中死区的影响,提升自主行驶的控制效果。 相似文献
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Ming Xie 《Computers & Electrical Engineering》1995,21(6):441-452
This paper presents an integrated approach to the problem of AGV guidance using trinocular vision. Although this method uses three cameras, we will not do any processings related to stereo matching nor 3-D reconstruction. Instead, our trinocular vision is divided into two subsystems: monocular vision for path localization and binocular vision for obstacle detection. First, we demonstrate the geometric principle of 2-D vision. Then, we show its application for the task of AGV's path localization as well as the task of AGV's obstacle detection in the framework of trinocular vision. Experimental results with real images prove the usefulness of our method. 相似文献
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设计了具有非接触自主无线充电功能的室内移动机器人系统,采用基于磁耦合谐振原理的无线电能传输技术对机器人进行非接触无线充电。发射线圈埋于室内固定某处,其上方放置扬声器,接收线圈安放在机器人底部。充电步骤为:机器人通过监测电池电压或用户发送手机短信的方式开启充电程序,同时触发能量发射系统语音播放单元,使其播放语音指令呼叫机器人充电;机器人利用DSP实时采集语音信号,并通过声源定位算法判断发射系统方向,利用DSP进行驱动控制,指导其寻找发射源;当机器人进入发射源指定区域范围内,便可实现无线充电。该系统具有自主移动寻源和非接触无线充电的特点,无需人为干预,机器人可在几十秒内快速找到发射源并实现自主无线充电。 相似文献
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基于生物行为模拟的知识处理系统研究 总被引:2,自引:0,他引:2
文章分析了新型知识处理系统所具有的自主性和创新性,提出了基于生物行为模拟的知识处理系统的概念,构建了相应的系统框架模型,并阐述了其功能单元构成和组织体系结构,对系统实现过程中所应面临的技术难题进行了初步探讨。 相似文献
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Sven Müller Thomas Sandal Peter Kamp-Hansen Henrik Dalbge 《Yeast (Chichester, England)》1998,14(14):1267-1283
We have compared expression systems based on autonomously replicating vectors in the yeasts Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Hansenula polymorpha and Yarrowia lipolytica in order to identify a more suitable host organism for use in the expression cloning method (Dalbøge and Heldt-Hansen, 1994) in which S. cerevisiae has traditionally been used. The capacity of the expression systems to secrete active forms of six fungal genes encoding the enzymes galactanase, lipase, polygalacturonase, xylanase and two cellulases was examined, as well as glycosylation pattern, plasmid stability and transformation frequency. All of the examined alternative hosts were able to secrete more active enzyme than S. cerevisiae but the relative expression capacity of the individual hosts varied significantly in a gene-dependent manner. One of the most attractive of the alternative host organisms, Y. lipolytica, yielded an increase which ranged from 4·5 times to more than two orders of magnitude. As the initially employed Y. lipolytica XPR2 promoter is unfit in the context of expression cloning, two novel promoter sequences for highly expressed genes present in only one copy on the genome were isolated. Based on sequence homology, the genes were identified as TEF, encoding translation elongation factor-1α and RPS7, encoding ribosomal protein S7. Using the heterologous cellulase II (celII) and xylanase I (xylI) as reporter genes, the effect of the new promoters was measured in qualitative and quantitative assays. Based on the present tests of the new promoters, Y. lipolytica appears as a highly attractive alternative to S. cerevisiae as a host organism for expression cloning. GenBank Accession Numbers: TEF gene promoter sequence: AF054508; RPS7 gene promoter sequence: AF054509. © 1998 John Wiley & Sons, Ltd. 相似文献
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叶雯 《数字社区&智能家居》2007,1(6):1770-1771
学习活动不是在一个设计好的、以教师为中心的环境里获取信息的过程。当学生对学习产生了浓厚的兴趣,当他们切实参与了各项学习活动时,他们的学习效率就会得到很大提高。对在信息化的教学环境下,高校师生如何充分利用各种资源,进行高效教学活动,创建一种基于多媒体作品制作的教学方式进行了研究。 相似文献
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