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1.
Matej Vizovisek Dragana Ristanovic Stefano Menghini Michael G. Christiansen Simone Schuerle 《International journal of molecular sciences》2021,22(5)
In recent decades, dysregulation of proteases and atypical proteolysis have become increasingly recognized as important hallmarks of cancer, driving community-wide efforts to explore the proteolytic landscape of oncologic disease. With more than 100 proteases currently associated with different aspects of cancer development and progression, there is a clear impetus to harness their potential in the context of oncology. Advances in the protease field have yielded technologies enabling sensitive protease detection in various settings, paving the way towards diagnostic profiling of disease-related protease activity patterns. Methods including activity-based probes and substrates, antibodies, and various nanosystems that generate reporter signals, i.e., for PET or MRI, after interaction with the target protease have shown potential for clinical translation. Nevertheless, these technologies are costly, not easily multiplexed, and require advanced imaging technologies. While the current clinical applications of protease-responsive technologies in oncologic settings are still limited, emerging technologies and protease sensors are poised to enable comprehensive exploration of the tumor proteolytic landscape as a diagnostic and therapeutic frontier. This review aims to give an overview of the most relevant classes of proteases as indicators for tumor diagnosis, current approaches to detect and monitor their activity in vivo, and associated therapeutic applications. 相似文献
2.
目的 旅游服务设计发展态势良好,从旅游服务设计的角度进行文献可视化分析,可从中获得当前领域的知识结构、研究热点和发展态势,为未来提供旅游服务设计研究和旅游产业的服务设计参考。方法 从CNKI和Web of Science数据库收集1990—2020年国内外的相关数据,运用CiteSpace对旅游服务设计的文献发表时间、高度被引用的文献、研究热点和研究前沿进行可视化分析,比较国内外研究差异。结论 在1990—2020年国内外旅游服务设计研究皆呈上升趋势。国外的研究热度、被引频次高于国内,以跨学科领域研究尤为突出,研究重点在建筑环境和交通相关策略;而国内研究以产品服务系统为主,偏向于技术的实现方式。综观研究现状和趋势,本文从智慧科技旅游、可持续发展和全域旅游进行深入探讨,提出旅游服务设计新发展趋势。 相似文献
3.
《Journal of dairy science》2022,105(6):5471-5492
The detection of reproductive tract disease (RTD) 3 wk postpartum is important because of its effect on subsequent reproductive outcomes. Numerous methods for the diagnosis of RTD are described, some of which are more practical and instantaneous in terms of diagnosis. Two of these methods involve identification of purulent vaginal discharge (PVD) and evidence of ultrasonographic uterine changes indicative of endometritis (UE). The objectives of our retrospective observational study were (1) to assess the association of PVD or UE score at the prebreeding examination (PBE) with the hazard of pregnancy within the subsequent breeding season; (2) to determine the test sensitivity (Se) and specificity (Sp) at the point of sampling of both tests using a Bayesian latent class model; and (3) to determine the effect of varying positivity thresholds on test accuracy. To achieve these objectives, we analyzed an initial data set of 5,049 PBE from 2,460 spring-calved cows in 8 herds between 2014 and 2018. Each PBE was conducted once between 25 and 86 d in milk. At each PBE, vaginal discharge was obtained with a Metricheck device (Simcro) whereas uterine contents were assessed using transrectal ultrasonography. Purulent vaginal discharge was scored on a scale of 0 to 3 depending on discharge character, and UE was scored on a scale of 0 to 4 depending on the presence and consistency of intraluminal fluid. Cows with scores of ≥2 in either test had received treatment. Fertility data were available from 4,756 PBE after data exclusion. The association between PVD or UE score at the PBE and subsequent hazard of pregnancy was analyzed using a Cox proportional hazards model. Cows with a PVD score of 2 or 3 were less likely to conceive than cows with a PVD score 0 [score 2 hazard ratio (HR) = 0.74; 95% confidence interval (CI): 0.59–0.94; score 3 HR = 0.65; 95% CI: 0.51–0.84]. Cows with a UE score of 1, 2, 3, or 4 were less likely to conceive than cows with a UE score of 0 (score 1 HR = 0.82; 95% CI: 0.73–0.93; score 2 HR = 0.79; 95% CI: 0.62–1.00; score 3 HR = 0.43; 95% CI: 0.43–0.90; score 4 HR = 0.39; 95% CI: 0.26–0.58). To determine the Se and Sp of PVD or UE score for diagnosis of RTD at the time of PBE, a Bayesian latent class model was fitted on 2,460 individual cow PBE. Flat priors were used for the Se and Sp of UE, whereas informative priors were used for PVD Se (mode = 65%, 5th percentile = 45%) and Sp (mode = 90%, 5th percentile = 80%) and RTD prevalence (mode = 20%, 5th percentile = 10%). Posterior estimates (median and 95% Bayesian probability intervals; BPI) were obtained using ‘rjags' (R Studio). The optimal test thresholds (PVD and UE score ≥1) were selected by assessing the effect of different thresholds on test estimates and using a misclassification cost analysis. Based on these, median (95% BPI) Se for PVD and UE score ≥1 were 44% (29–60%) and 67% (33–100%), respectively. Median Sp for PVD and UE score ≥1 were 90% (86–93%) and 91% (86–93%), respectively. Higher scores in both tests were associated with impaired fertility, and UE scoring with a threshold of ≥1 had the highest test Se and Sp estimates although test Se was conditional on days in milk when the PBE occurred. 相似文献
4.
目的 将数字化服务融入老年患者就诊流程,建立适老化就诊等待服务系统,在APP开发设计研究中实现软件流程优化。方法 依据ERG理论对老年患者在就诊等待过程中的需求点进行分类整理和层次划分。调研目标用户将分析结果融入服务设计理论,为指导软件开发所涉及的医院就诊流程、用户需求痛点,提出系统性的解决策略。基于交互设计原则展开APP界面设计。结论 构建了以老年患者为中心的就诊等待服务系统,帮助提升其等待过程中的自我效能。完善了基于产品使用方式层级的适老化就诊等待服务APP设计策略,为适老化、数字化产品研发提供了新思路;最终产出APP设计实例,提高了老年群体社会参与度,鼓励老年患者自主就诊,帮助其更加轻松地享受信息化时代带来的红利。 相似文献
5.
One of the most challenging issues in radio received signal strength (RSS)-based localization systems is the generation and distribution of a radio map with a coordinate system linked with spatial information in a large indoor space. This study proposes a novel spatial-tagged radio-mapping system (SRS) that effectively combines the heterogeneous properties of LiDAR and mobile phones to simultaneously perform both spatial and radio mappings. The SRS consists of synchronization, localization, and map building processes, and enables real-time spatial and radio mapping. In the synchronization process, the distance range, motion data, and radio signals obtained through the LiDAR and mobile phone are collected in nodal units according to the sensing time. In the localization process, a feature variance filter is used to control the number of features generated from LiDAR and estimate the positions at which the nodes are generated in real time according to the motion data and radio signals. In map building, the estimated positions of the nodes are used to extract spatial and radio maps by using a unified location coordinate system. To ensure mobility, the SRS is manufactured in the form of a backpack supporting LiDAR and a mobile phone; the usefulness of the system is experimentally verified. The experiments are performed in a large indoor shopping mall with a complex structure. The experimental results demonstrated that a common coordinate system could be used to build spatial and radio maps with high accuracy and efficiency in real time. In addition, the field applicability of the SRS to location-based services is experimentally verified by applying the constructed radio map to well-known fingerprinting algorithms using the heterogeneous mobile phones. 相似文献
6.
摘 要:核心网业务模型的建立是5G网络容量规划和网络建设的基础,通过现有方法得到的理论业务模型是静态不可变的且与实际网络存在偏离。为了克服现有5G核心网业务模型与现网模型适配性较差以及规划设备无法满足用户实际业务需求的问题,提出了一种长短期记忆(long short-term memory,LSTM)网络与卷积LSTM (convolution LSTM,ConvLSTM)网络双通道融合的 5G 核心网业务模型预测方法。该方法基于人工智能(artificial intelligence,AI)技术以实现高质量的核心网业务模型的智能预测,形成数据反馈闭环,实现网络自优化调整,助力网络智能化建设。 相似文献
7.
在广电总局和省局指示精神的指导下,江苏有线三网融合枢纽中心建成了省中心集中汇聚,分中心独立管控的三级安播监管体系。本文从建设目标,网络结构,系统组成等方面介绍了江苏有线安播监管系统,提出了“播监控三维一体”,“省市县三位一体”的省级全覆盖式安播监管体系解决方案。 相似文献
8.
Fangqing Wei Xiaoqi Zhang Jiayun Song Feng Yang 《International Transactions in Operational Research》2023,30(1):369-393
Evaluating the efficiency of healthcare services accurately can help in analyzing the rationality of inputs and outputs in such services. Considering the consistency and equity of assessment criteria, this study conducts the stochastic multicriteria acceptability analysis (SMAA-2) with a directional distance function to evaluate the efficiency of healthcare services in 31 provincial administrative regions of mainland China, as observed in 2018. We use SMAA-DDF to explore all the projection directions to the efficient frontier instead of a certain projection direction. We measure the maximum and average efficiencies for each of the 31 provincial healthcare services. Our empirical findings show that only seven provinces achieve optimal healthcare service efficiency; the eastern area performed the best, followed by the central, western, and northeast areas. Furthermore, the path along the projection directions is provided to help inefficient provinces improve their efficiency and obtain the best possible positions. 相似文献
9.
Yixuan ZHOU Huajie WANG Yi YU Yihang CHEN Rui KE Min XU Chijie XIAO Zhanhui WANG Jiquan LI Xuru DUAN Minyou YE 《等离子体科学和技术》2021,23(7):75105-52
In this article, we present the optical design of a novel diagnostic on the HL-2 A tokamak, i.e. the20-channel edge Lyman-alpha beam emission spectroscopy, which is a promising solution for edge density turbulence research on tokamaks, as it offers the possibility of density fluctuation measurement with a 3.3 mm spatial resolution while maintains a high temporal resolution of1 μs. The optical path, including the reflective collection optics, the high-dispersion spectrometer, and the linear detector array, is carefully optimized to obtain a good image quality and a high throughput. The maximum root mean square radius of the collection optics is 64 μm.The detected photon flux is estimated to be about 10~(11) photons/s/channel. 相似文献
10.