Spontaneous Cell Detachment and Reattachment in Cancer Cell Lines: An In Vitro Model of Metastasis and Malignancy

There is an unmet need for simplified in vitro models of malignancy and metastasis that facilitate fast, affordable and scalable gene and compound analysis. “Adherent” cancer cell lines frequently release “free-floating” cells into suspension that are viable and can reattach. This, in a simplistic way, mimics the metastatic process. We compared the gene expression profiles of naturally co-existing populations of floating and adherent cells in SW620 (colon), C33a (cervix) and HeLa (cervix) cancer cells. We found that 1227, 1367 and 1333 genes were at least 2-fold differentially expressed in the respective cell lines, of which 122 were shared among the three cell lines. As proof of principle, we focused on the anti-metastatic gene NM23-H1, which was downregulated both at the RNA and protein level in the floating cell populations of all three cell lines. Knockdown of NM23-H1 significantly increased the number of floating (and viable) cells, whereas overexpression of NM23-H1 significantly reduced the proportion of floating cells. Other potential regulators of these cellular states were identified through pathway analysis, including hypoxia, mTOR (mechanistic target of rapamycin), cell adhesion and cell polarity signal transduction pathways. Hypoxia, a condition linked to malignancy and metastasis, reduced NM23-H1 expression and significantly increased the number of free-floating cells. Inhibition of mTOR or Rho-associated protein kinase (ROCK) significantly increased cell death specifically in the floating and not the adherent cell population. In conclusion, our study suggests that dynamic subpopulations of free-floating and adherent cells is a useful model to screen and identify genes, drugs and pathways that regulate the process of cancer metastasis, such as cell detachment and anoikis.     

热效应影响下浮环轴承润滑静特性研究

建立计入热效应的浮环轴承流体动压润滑模型,利用数值差分法联立求解雷诺方程,进而求解浮环轴承端泄流量;通过与实验数据的对比,验证所建模型的正确性;考虑浮环受热膨胀变形的影响,研究供油温度对摩擦功耗、温升、浮动环转速、端泄流量等浮环轴承润滑静特性参数的影响。结果表明:提高润滑油供油温度会明显提升浮动环旋转速度,增大轴承内间隙的供油量,减小内外油膜摩擦功耗和端泄温升;端泄温升随轴颈转速的升高而增大,温升随轴颈转速变化的幅度在采用较低温度的润滑油时表现得更为剧烈。     

雷达发射机浮动板调制器与故障检测电路的设计

提出了一种改进的浮动板调制器和对正负偏电压的故障检测电路,利用MOSFET寄生电容特性,通过固定脉宽窄脉冲控制调制脉宽,通过增加负偏MOSFET提高输出负偏电压。对故障检测电路进行仿真实验,通过模拟故障,验证故障检测报警信号发生时间均在微秒级别。完成样机的搭建,利用多重方式解决高压绝缘问题,并对其加电进行试验验证,分析实验波形,证明此电路满足设计要求并且具有可行性。     

极端海况下浮式防波堤的水动力性能分析

基于三维势流理论,以消波性能形式较好的双圆筒浮式防波堤为研究对象,通过有限元软件Workbench建立不规则波作用下浮式防波堤的湿表面模型,利用水动力分析软件AQWA对浮式防波堤进行频域水动力性能分析和时域全耦合分析,重点研究浮式防波堤在极端海况下的响应幅值算子、运动响应和系泊缆张力,为相关实际工程提供参考。     

6 MW单柱型浮式风力机系泊链预张力对运动响应特性的影响

以6 MW海上单柱浮式风力机为研究对象,运用时域耦合分析方法探讨系泊链水平预张力对浮式风力机系统整体运动响应特性的影响。数值研究结果表明:增加系泊链水平预张力能有效改善浮式风力机的纵荡和垂荡运动,对浮式风力机纵摇和横摇运动的影响有限。根据系泊链预张力对浮式风力机整体运动的影响特性优化系泊系统,优化后的浮式风力机具备良好的总体运动响应,该系泊系统能保证浮式风力机的安全。所得结论对浮式风力机系泊系统设计具备一定参考意义。     

Review of Antiswing Control of Shipboard Cranes

Shipboard cranes are extensively utilized in numerous fields such as cargo transferring and offshore engineering. The control of shipboard cranes, especially the antiswing control of payloads, has attracted much research attention due to their typical underactuation characteristics and complicated dynamics. Through comparisons of the traditional land-fixed cranes, a brief review on modeling and dynamics analysis is presented to illustrate the tremendous challenges and difficulties in controller design for shipboard cranes. A comprehensive review and brief analysis of shipboard crane control strategies are further presented. Some future research directions are also put forward for reference. It is expected that the paper will be useful for improving existing control schemes and generating novel control approaches for shipboard crane systems.      

基于CATIA的桥式起重机司机室人机工程设计

为改善桥式起重机司机室作业环境,提高作业舒适度,对桥式起重机司机室的布局及各尺寸结合人机工程学相关理论进行了分析,并给出了尺寸建议,最后以人机工程虚拟仿真软件CATIA进行验证,分析了司机的视野范围、操作姿势舒适度和手部可达区域。结果表明结合人机工程学相关理论所设计的司机室符合人体舒适度要求,设计中加入了视频监控装置,可降低职业病发生的可能性,司机室尺寸优化结果可为起重机司机室设计提供重要的数据参考。     

波浪荷载作用下海上新型潜式浮式风力机运动特性研究

以适用于中等水深(50~200 m)的新型潜式浮式风力机为研究对象,该风力机基础综合Spar式、半潜式及张力腿(TLP)3类浮式风力机基础的优点,运行时基础主体淹没在水下,具有较小的水线面(如同Spar平台),受波浪影响较小;平台通过张紧式系泊线与海床相连(如同TLP平台),具有良好的垂荡和摇摆运动特性;拖航状态下,浮式平台处于半潜状态,水线面面积大(如同半潜式平台),具有良好的浮稳性。通过分析不同波况下的潜式浮式风力机耦合动力响应得到潜式浮式基础的横荡、纵荡、垂荡及纵摇运动响应,以及发电功率、叶片根部弯矩、塔筒顶部和底部弯矩、锚链张力时程曲线。研究结果表明:波浪对于结构的纵摇运动的影响最为明显,对发电功率、叶片根部弯矩和塔筒顶部弯矩影响较小,对塔筒底部弯矩和系泊线张力影响较大。     

Numerical modeling of the hydraulic blade pitch actuator in a spar‐type floating wind turbine considering fault conditions and their effects on global dynamic responses

This paper deals with numerical modeling of the hydraulic blade pitch actuator and its effect on the dynamic responses of a floating spar‐type wind turbine under valve fault conditions. A spar‐type floating wind turbine concept is modeled and simulated using an aero‐hydro‐servo‐elastic simulation tool (Simo‐Riflex [SR]). Because the blade pitch system has the highest failure rate, a numerical model of the hydraulic blade pitch actuator with/without valve faults is developed and linked to SR to study the effects of faults on global responses of the spar‐type floating wind turbine for different faults, fault magnitudes, and environmental conditions. The consequence of valve faults in the pitch actuator is that the blade cannot be pitched to the desired angle, so there may be a delay in the response due to excessive friction and the wrong voltage, or slit lock may cause runaway blade pitch. A short circuit may cause the blade to get stuck at a particular pitch angle. These faults contribute to rotor imbalance, which result in different effects on the turbine structure and the platform motions. The proposed method for combining global and hydraulic actuator models is demonstrated in case studies with stochastic wind and wave conditions and different types of valve faults.     

起重机自重应力对安全评估中应力测试实验结果的影响

应力测试是起重机安全评估中必须进行的一项检测。目前在安全评估工作中常采用应变电测法来采集起重机工作过程中的应力时程数据,由于该方法只能采集到结构由于载荷变化产生的应力,而无法采集到结构因自重产生的应力,导致应力测试无法获取结构的完整应力。利用起重机金属结构自重应力在总应力中占有的权重,分析其对应力测试实验结果的影响大小,试验结果表明在危险截面自重应力大小占总应力大小可达60%,此时不考虑自重应力进行结构安全性的判定是不全面的。可考虑利用仿真技术获取自重应力[1,2],从而使得安全评估应力测试实验结论更加准确。