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1.
Sobha Karuthedom George Lucia Laukov Ren Weiss Vladislav Semak Birgit Fendl Victor U. Weiss Stephanie Steinberger Günter Allmaier Carla Tripisciano Viktoria Weber 《International journal of molecular sciences》2021,22(8)
Growing interest in extracellular vesicles (EVs) has prompted the advancements of protocols for improved EV characterization. As a high-throughput, multi-parameter, and single particle technique, flow cytometry is widely used for EV characterization. The comparison of data on EV concentration, however, is hindered by the lack of standardization between different protocols and instruments. Here, we quantified EV counts of platelet-derived EVs, using two flow cytometers (Gallios and CytoFLEX LX) and nanoparticle tracking analysis (NTA). Phosphatidylserine-exposing EVs were identified by labelling with lactadherin (LA). Calibration with silica-based fluorescent beads showed detection limits of 300 nm and 150 nm for Gallios and CytoFLEX LX, respectively. Accordingly, CytoFLEX LX yielded 40-fold higher EV counts and 13-fold higher counts of LA+CD41+ EVs compared to Gallios. NTA in fluorescence mode (F-NTA) demonstrated that only 9.5% of all vesicles detected in scatter mode exposed phosphatidylserine, resulting in good agreement of LA+ EVs for CytoFLEX LX and F-NTA. Since certain functional characteristics, such as the exposure of pro-coagulant phosphatidylserine, are not equally displayed across the entire EV size range, our study highlights the necessity of indicating the size range of EVs detected with a given approach along with the EV concentration to support the comparability between different studies. 相似文献
2.
Tracking-by-detection (TBD) is a significant framework for visual object tracking. However, current trackers are usually updated online based on random sampling with a probability distribution. The performance of the learning-based TBD trackers is limited by the lack of discriminative features, especially when the background is full of semantic distractors. We propose an attention-driven data augmentation method, in which a residual attention mechanism is integrated into the TBD tracking network as supplementary references to identify discriminative image features. A mask generating network is used to simulate changes in target appearances to obtain positive samples, where attention information and image features are combined to identify discriminative features. In addition, we propose a method for mining hard negative samples, which searches for semantic distractors with the response of the attention module. The experiments on the OTB2015, UAV123, and LaSOT benchmarks show that this method achieves competitive performance in terms of accuracy and robustness. 相似文献
3.
Barbora Peltanova Marketa Liskova Jaromir Gumulec Martina Raudenska Hana Holcova Polanska Tomas Vaculovic David Kalfert Marek Grega Jan Plzak Jan Betka Michal Masarik 《International journal of molecular sciences》2021,22(4)
Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigated the ability of head and neck squamous cell carcinomas (HNSCC) patient-derived CAFs to promote or inhibit the colony-forming ability of HNSCC cells. The effect of cisplatin on this promoting or inhibiting influence was also studied. The subsequent analysis focused on changes in the expression of genes associated with cancer progression. We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different “sensitizing ratio”. Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. On the other hand, CCL2 expression was associated with sensitizing effect. Significantly higher amounts of cisplatin were found in CAFs derived from patients who subsequently experienced a recurrence. In conclusion, our results showed that CAFs could promote and/or inhibit colony-forming capability and cisplatin resistance in HNSCC cells via paracrine effects and subsequent changes in gene expression of cancer-associated genes in cancer cells. 相似文献
4.
针对当前轧钢企业智能工厂及智能物流的建设,介绍了“黑灯库房”在山钢日照公司冷轧厂轧后库和成品库的应用情况;主要对无人行车的硬件组成及关键技术,如防碰撞技术、智能扫描定位系统、吊具防摇摆技术、智能吊具系统等,进行了详细阐述,并对库管系统及其功能进行了介绍。实际应用表明,采用“黑灯库房”不仅能够带来良好的经济效益,而且库存周转更快、更高效,在安全上提供了更强有力的保障,使山钢日照公司智能制造水平有了较大提升,为下一步智能梦工厂的实现打下了良好的基础。 相似文献
5.
从发电原理和平准化度电成本角度来看,光伏是未来最有发展潜力的新能源利用方式。作为光伏发电系统的关键设备之一,至少需要从成本和可靠性两个维度来理解跟踪支架产品的特点。文章根据现有支架行业主流企业的产品特点,对跟踪支架的发展趋势进行预测。 相似文献
6.
气藏平均地层压力跟踪计算新方法 总被引:1,自引:0,他引:1
平均地层压力是产能评价和动态分析的基础,准确、快速获取平均地层压力对高效开发气藏意义重大。基于地层压力随时间变化的规律,分析了平均地层压力的变化规律。研究结果表明:平均地层压力等效点仅随时间发生改变,平均地层压力的下降速率等于或者近似等于井底流压的下降速率。从封闭弹性驱动气藏的物质平衡方程出发,考虑偏差系数和井底流压随平均地层压力的变化,推导建立了平均地层压力跟踪计算新方法,根据生产数据可迭代计算平均地层压力。方法验证结果显示,采气速度和采出程度共同影响模型的计算结果。应用实例表明,跟踪计算法与压力恢复试井和物质平衡法之间的相对误差均较小,满足工程计算精度要求,且跟踪计算法不需依托生产测试数据,节约了测试费用,避免了测试占产。 相似文献
7.
8.
为解决目标快速运动时跟踪算法出现目标丢失和跟踪精度大幅度下降等问题,在现有的Autotrack算法基础上对其进行改进,提出了一种基于快速运动场景下的目标跟踪算法。引入空间正则权重项w对距离目标中心比较远的样本进行相应的惩罚,调整原本的全局响应变化量并将其作为时间正则项。将空间正则项和时间正则项相结合,并引入目标函数中进行优化。在公开数据集OTB-2013(online object tracking:a benchmark)上对改进后的目标跟踪算法进行实验验证和比较。实验结果表明,改进后的目标跟踪算法在目标快速运动场景下的准确率和成功率分别为76.5%和73.1%,在综合评分上的准确率和成功率分别为82.8%和61.1%。 相似文献
9.
为了帮助服装设计师在设计更契合消费者审美需求的服饰图案时提供理论参考和数据支撑,本文采用眼动仪跟踪技术结合Like喜好程度主观测评表及SPSS.22.0数据分析软件,系统研究了同款T恤衫,不同图案色彩、大小、密度、角度及底色等服饰图案变量对消费者喜好程度的制约关系。结果表明:服饰图案整体设计效果中众多变量均会不同程度的影响消费者喜好程度;同时还发现,消费者喜好与消费者面对带有图案服装的关注时间和关注点数量呈正向关系。其结论可为服装设计师设计出更易被消费者认可的服装产品提供理论指导和方法借鉴。 相似文献
10.
This paper aims to provide a review of the conceptual design and theoretical framework of the main control schemes proposed in the literature for unmanned underwater vehicles (UUVs). Additionally, the objective of the paper is not only to present an overview of the recent control architectures validated on UUVs but also to give detailed experimental-based comparative studies of the proposed control schemes. To this end, the main control schemes, including proportional–integral–derivative (PID) based, sliding mode control (SMC) based, adaptive based, observation-based, model predictive control (MPC) based, combined control techniques, are revisited in order to consolidate the principal efforts made in the last two decades by the automatic control community in the field. Besides implementing some key tracking control schemes from the classification mentioned above on Leonard UUV, several real-time experimental scenarios are tested, under different operating conditions, to evaluate and compare the efficiency of the selected tracking control schemes. Furthermore, we point out potential investigation gaps and future research trends at the end of this survey. 相似文献