南美白对虾肝胰腺蛋白提取方法优化及无水保活环境胁迫对细胞凋亡蛋白的影响

为提高Western Blotting结果的可靠性，以蛋白溶出率和凝胶电泳蛋白条带完整性为指标，比较提取液种类、研磨方式、酶抑制剂种类及其体积分数和提取时间对南美白对虾肝胰腺蛋白提取效果的影响。采用优化后的提取方法获得高质量蛋白样品，并采用Western Blotting法分析无水环境胁迫后南美白对虾肝胰腺组织中细胞凋亡信号通路相关蛋白的表达水平。结果表明：RIPA裂解液作为提取溶剂所得的蛋白溶出率高于水提和磷酸盐缓冲液，电动匀浆和液氮研磨所得蛋白条带更完整，4%蛋白酶磷酸酶混合抑制剂能有效抑制肝胰腺内源酶引起的蛋白降解；采用Western Blotting法分析无水保活期间南美白对虾肝胰腺蛋白，发现低温诱导休眠的同时会引起细胞轻微凋亡，且凋亡水平呈应激时间依赖性增加，环境胁迫解除后有所回调。     

2019年黑龙江省猪肉市场运行情况分析

2018年非洲猪瘟疫情出现以来,全国及黑龙江省生猪生产、猪肉市场受到了较大的影响,生猪、能繁母猪存栏减少,生猪出场价格也经历了明显波动。2019年春节以后,随着省外调出量增加和猪源减少,黑龙江省生猪出场价格进入上升通道,超过了全国平均水平,白条猪肉批发价格和猪肉市场零售价格随之上行。通过对2019年黑龙江省生猪生产环节、屠宰加工环节、猪肉市场零售环节进行调研,对生猪出场价格、白条猪肉批发价格、猪肉零售价格、生猪和能繁母猪产能等指标进行分析,阐述了以生猪和猪肉市场为重点的肉类产销形势,为促进肉类产业高质量发展、提高市场保供能力提供参考。     

Adaptive neuro-fuzzy algorithm applied to predict and control multi-phase flow rates through wellhead chokes

A Takagi-Sugeno adaptive neuro-fuzzy inference system (TSFIS) model is developed and applied to a dataset of wellhead flow-test data for the Resalat oil field located offshore southern Iran, the objective is to assist in the prediction and control of multi-phase flow rates of oil and gas through the wellhead chokes. For this purpose, 182 test data points (Appendix 1) related to the Resalat field are evaluated. In order to predict production flow rate (Q_L) expressed as stock-tank barrels per day (STB/D), this dataset includes four selected input variables: upstream pressure (Pwh); wellhead choke sizes (D64); gas to liquid ratio (GLR); and, base solids and water including some water-soluble oil emulsion (BS&W). The test data points evaluated include a wide range of oil flow rate conditions and values for the four input variables recorded. The TSFIS algorithm applied involves five data processing steps: a) pre-processing, b) fuzzification, c) rules base and adaptive neuro-fuzzy inference engine, d) defuzzification, and e) post-processing of the fuzzy model. The developed TSFIS model for the Resalat oil field database predicted oil flow rate to a high degree of accuracy (root mean square error = 247 STB/D, correlation coefficient = 0.9987), which improves substantially on the commonly used empirical algorithms used for such predictions. TSFIS can potentially be applied in wellhead choke fuzzy controllers to stabilize flow in specific wells based on real-time input data records.     

An engineering method for complex structural optimization involving both size and topology design variables

This work presents an engineering method for optimizing structures made of bars, beams, plates, or a combination of those components. Corresponding problems involve both continuous (size) and discrete (topology) variables. Using a branched multipoint approximate function, which involves such mixed variables, a series of sequential approximate problems are constructed to make the primal problem explicit. To solve the approximate problems, genetic algorithm (GA) is utilized to optimize discrete variables, and when calculating individual fitness values in GA, a second-level approximate problem only involving retained continuous variables is built to optimize continuous variables. The solution to the second-level approximate problem can be easily obtained with dual methods. Structural analyses are only needed before improving the branched approximate functions in the iteration cycles. The method aims at optimal design of discrete structures consisting of bars, beams, plates, or other components. Numerical examples are given to illustrate its effectiveness, including frame topology optimization, layout optimization of stiffeners modeled with beams or shells, concurrent layout optimization of beam and shell components, and an application in a microsatellite structure. Optimization results show that the number of structural analyses is dramatically decreased when compared with pure GA while even comparable to pure sizing optimization.     

环锭纱络筒质量的检测与控制

为探讨环锭纱络筒过程中纱线质量的影响因素,通过设置对比实验的方法测试络筒纱线的毛羽数、条干不匀率、强伸性和卷绕点分布,得到同一纱筒上不同半径纱层的纱线质量数据,并进行研究分析。结果表明:中层筒子纱线的毛羽较少,对应的纱线比较光滑,CV值较低,条干均匀度高;最内层和最外层纱线的强力最大;最外层纱线重叠率最低。生产中可以采取改进络筒工艺参数、选择适宜的槽筒材质等措施,提高卷绕质量及络筒质量。     

Empirical examination of critical failure factors of continuous improvement deployments: stage-wise results and a contingency theory perspective

Organisations implement various Continuous Improvement (CI) practices such as Total Quality Management, Lean, Six Sigma and Lean Six Sigma (LSS) for improving their processes. Drawing from the success and failure stories of these structured CI practices, scholars enumerated Critical Success Factors and Critical Failure Factors (CFFs). This study empirically examines the occurrence of various CFFs across different stages of CI deployment. Further, from a contingency theoretic perspective, this study investigates their associations with contextual variables by collecting survey data from 213 business units from the USA, the UK, China, and India. Principal Component Analysis is used to group CFFs across five CI deployment stages leading to an empirically refined framework for CI. Crosstab analysis using the chi-square likelihood ratio presented associations of CFFs with contextual variables. Findings reveal significant differences in the occurrence of CFFs across countries. There is evidence that LSS is less prone to failures when compared with TQM, Lean and Six Sigma. The occurrence of CFFs has been relatively lower in smaller and medium-sized enterprises operating in the service sector. Findings also reveal that lessons learned from each CI deployment cycle, contribute to organisational learning, and thence, leading to success at the strategic CI level of maturity.     

Generalized F-discrepancy-based point selection strategy for dependent random variables in uncertainty quantification of nonlinear structures

In the performance evaluation of structures under disastrous actions, for example, earthquakes, it is important to take into account the randomness of structural parameters. Generally, these random parameters are treated either as independent or perfectly dependent, but practically they are partly dependent. This article aims at developing a point selection strategy for uncertainty quantification of nonlinear structures involving probabilistically dependent random parameters characterized by copula function. For this purpose, the point selection strategy for structures involving independent basic variables is first revisited. As an improvement, a generalized F-discrepancy diminishing oriented iterative screening algorithm is proposed. Then, combining with the conditional sampling method, a conditional point set rearrangement method and a conditional iterative screening-rearrangement method are proposed for probabilistically dependent variables. These new point selection strategies are readily incorporated into the probability density evolution method for uncertainty quantification of nonlinear structures involving dependent random parameters, which is characterized by copula function. The proposed methods are illustrated by two examples including a shear frame with hysteretic restoring forces and a reinforced concrete frame structure with the damage constitutive model of concrete, where the material parameters are probabilistically dependent. The results demonstrate the effectiveness of the proposed method. Problems to be studied are discussed.     

Sensor network design based on system-wide reliability criteria. Part I: Objectives

Reliability based criteria are quite popular for optimal sensor network design. We present a modified definition of system reliability for sensor network design for two applications: reliable estimation of variables in a steady state linear flow process, and reliable fault detection and diagnosis for any process. Unlike the weakest-link based definition of system reliability in the literature, the proposed definition considers the entire system and is consistent with the reliability concept used in classical reliability literature. For each application, dual approaches for defining system reliability are proposed, and their analogy with the reliability problem in the classical reliability literature is established. Using examples and stochastic simulations, the advantage of using the proposed system reliability in contrast to the existing definition is illustrated. Part II of this series of articles presents methods for efficient generation of the system reliability function and its use in optimization-based approaches for designing optimal sensor networks.     

Benefits of variable structure techniques for parameter estimation in stochastic systems using least squares method and instrumental variables

In this paper, we study the benefits of the equivalent control method, implemented on parameter estimation algorithms like the least squares method and instrumental variables on continuous‐time stochastic systems. Here, we show how the equivalent‐control, adapted to the stochastic case, improves the performance of these estimation algorithms in continuous‐time systems under white noise perturbations. Some numerical examples show the effectiveness of these techniques. Copyright © 2014 John Wiley & Sons, Ltd.