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1.
Autofocusing for tissue microscopy   总被引:11,自引:0,他引:11  
This paper describes the implementation of autofocusing for tissue microscopy. We first investigate the suitability of several criterion functions for the evaluation of image sharpness. Since tissue sections are invariably stained, we also discuss the selection of the colour component on which autofocusing will be performed. In tissue microscopy, where a section generally comprises multiple layers, it is often not possible to obtain an image that is well focused over the field of view because of the limited depth of field of the objective. We describe focus enhancement algorithms, closely related to the autofocus system, which may be employed to obtain an entirely sharp image.  相似文献
2.
计算机三维物体实体组建方法的研究   总被引:3,自引:0,他引:3  
在计算机绘图和三维图像显示,例如激光扫描共焦显微图像、CT图像、MRI图像等的绘制和显示中,三维物体的组建是必不可少的。目前,三维物体的组建都是基于二维物体组建的基础之上。二维物体组建是基于连通性进行二维连通区域检出的,因而三维物体的组建速度比较慢。为此,文章提出一种新方法:围线积分法区域标号和链接表法三维叠片,用于三维图像中三维物体实体的组建。这种方法的基本原理是在三维二值图像中,先采用围线积分法区域标号,组建二维物体切片,再采用链接表法三维叠片,由二维物体切片组建三维实体。这种方法的优点是组建速度比较快。  相似文献
3.
STM三维图象处理在微机上的实现   总被引:1,自引:0,他引:1  
本文介绍了可在微机上运行的扫描隧道显微镜三维图象处理程序。程序运用平移、比例、旋转、透视等几何变换将样品表面形貌以适当的位置、大小和角度显示在二维屏幕上,经消隐和明暗处理加强图象的立体感、真实感和清晰度,通过颜色模型转换将图象以灰度或混合过渡颜色绘制在高分辨显示终端上,实现 STM 数据的三维立体显示,进一步加强仪器的数据处理和显示功能。  相似文献
4.
A color object recognition scheme: application to cellular sorting   总被引:1,自引:0,他引:1  
This paper presents a color object recognition scheme which proceeds in three sequential steps: segmentation, features extraction and classification. We mainly focus on the first and the third steps here. A color watershed using global and local criteria is first described. A color contrast value is defined to select the best color space for segmenting color objects. Then, an architecture of binary neural networks is described. Its properties relies on the simplification of the recognition problem, leading to a noticeable increase in the classification rate. We conclude with the abilities of such a recognition scheme and present an automated cell sorting system. Received: 15 June 2001 / Accepted: 1 July 2002 Published online: 23 April 2003 Correspondence to: O. Lezoray (e-mail: Olivier.Lezoray@info.unicaen.fr)  相似文献
5.
亚微米光纤生物化学传感器   总被引:1,自引:0,他引:1  
概述了新型业微米光纤生物化学传感器的原理、研制方法和应用现状,并对其发展进行了展望。  相似文献
6.
Live cell imaging: a computational perspective   总被引:1,自引:0,他引:1  
One of the primary challenges in understanding complex living systems requires a good understanding of the interactions between cellular and molecular functional units. Live cell imaging is the process of non-invasively analyzing dynamic processes in living cells using state-of-the-art microscopy and computer vision techniques. Live cell imaging research provides exciting and novel insights into cell biology. In this paper, we present an overview of live cell imaging research and detail the role of computational image processing in live cell imaging.
Sameer Singh (Corresponding author)Email:
  相似文献
7.
Manipulating DNA molecules in nanofluidic channels   总被引:1,自引:0,他引:1  
Nanofluidic channels of ∼40×60 nm (width × depth) were fabricated with focused ion beam (FIB) milling instrument on a silicon nitride (Si3N4) film. Stained λ-phage DNA molecules were driven into these open channels by capillary force and observed with fluorescence microscopy. The movements of DNA molecule in these channels were discussed. These sub-100 nm scale channels may be useful in studying single biomacromolecules.  相似文献
8.
Particle linear image velocimetry (PLIV), a novel velocity measurement method for microfluidic characterization, is reported. The method records a series of one-dimensional images that represent the trace of particles in the flow across a one-dimensional imager. Linear imaging results in a faster frame rate than planar imaging, allowing observations at larger magnifications, or the measurement of faster flow in real-time than comparable techniques. In contrast to particle image velocimetry (PIV), PLIV captures fast flow dynamics and does not require high-speed cameras or shutters. Furthermore, PLIV is adaptable to multiple linear imager formats and, as one example, can use laser scanning confocal microscopes (LSCMs) that acquire images slowly, but with high optical sectioning resolutions. A higher spatial resolution can be obtained for flows where the in-plane velocity gradient in the direction of the optical path (z direction) is important. The PLIV algorithm is fully described and its utility is demonstrated by the measurement of: a steady Poiseuille flow with 1-m spatial resolution in a microfluidic environment; dynamic measurement of transient flows with 250-ms temporal resolution; and the simultaneous calculation of particle dimension statistics.  相似文献
9.
Grading of astrocytomas is an important task for treatment planning; however, it suffers from significantly great inter-observer variability. Computer-assisted diagnosis systems have been propose to assist towards minimizing subjectivity, however, these systems present either moderate accuracy or utilize specialized staining protocols and grading systems that are difficult to apply in daily clinical practice. The present study proposes a robust mathematical formulation by integrating state-of-art technologies (support vector machines and least squares mapping) in a cascade classification scheme for separating low from high and grade III from grade IV astrocytic tumours. Results have indicated that low from high-grade tumours can be correctly separated with a certainty as high as 97.3%, whereas grade III from grade IV tumours with 97.8%. The overall performance was 95.2%. These high rates have been a result of applying the least squares mapping technique to features prior to classification. A significant byproduct of least squares mapping is that the number of support vectors of the SVM classifiers dropped dramatically from about 80% when no mapping was used to less than 5% when mapping was used. The latter is a clear indication that the SVM classifier has a greater potential to generalize well to new data. In this way, digital image analysis systems for automated grading of astrocytomas are brought closer to clinical practice.  相似文献
10.
调整EM算法结合加权小波在COSM中的应用   总被引:1,自引:0,他引:1       下载免费PDF全文
用基于深度变化成像模型的调整EM算法进行三维显微图像复原,不能更好地复原图像细节,而且耗时长。为提高图像的复原质量,缩短时间,提出把调整EM算法与加权小波相结合的算法。该算法先对加权小波系数进行调整,再用调整EM算法进行迭代复原。实验表明复原效果得到改善,并减少了迭代次数,效率明显提高。  相似文献
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