Labeling and Characterization of Phenol-Containing Glycopeptides Using Chemoselective Probes with Isotope Tags

Secondary metabolites are structurally diverse natural products (NPs) and have been widely used for medical applications. Developing new tools to enrich NPs can be a promising solution to isolate novel NPs from the native and complex samples. Here, we developed native and deuterated chemoselective labeling probes to target phenol-containing glycopeptides by the ene-type labeling used in proteomic research. The clickable azido-linker was included for further biotin functionalization to facilitate the enrichment of labeled substrates. Afterward, our chemoselective method, in conjunction with LC-MS and MSn analysis, was demonstrated in bacterial cultures. A vancomycin-related phenol-containing glycopeptide was labeled and characterized by our labeling strategy, showing its potential in glycopeptide discovery in complex environments.     

OWL‐based acquisition and editing of computer‐interpretable guidelines with the CompGuide editor

Computer‐Interpretable Guidelines (CIGs) are the dominant medium for the delivery of clinical decision support, given the evidence‐based nature of their source material. Therefore, these machine‐readable versions have the ability to improve practitioner performance and conformance to standards, with availability at the point and time of care. The formalisation of Clinical Practice Guideline knowledge in a machine‐readable format is a crucial task to make it suitable for the integration in Clinical Decision Support Systems. However, the current tools for this purpose reveal shortcomings with respect to their ease of use and the support offered during CIG acquisition and editing. In this work, we characterise the current landscape of CIG acquisition tools based on the properties of guideline visualisation, organisation, simplicity, automation, manipulation of knowledge elements, and guideline storage and dissemination. Additionally, we describe the CompGuide Editor, a tool for the acquisition of CIGs in the CompGuide model for Clinical Practice Guidelines that also allows the editing of previously encoded guidelines. The Editor guides the users throughout the process of guideline encoding and does not require proficiency in any programming language. The features of the CIG encoding process are revealed through a comparison with already established tools for CIG acquisition.     

Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

射频识别技术在钢管制造中的应用

介绍钢管管号识别方式,分析射频识别技术在钢管制作流程中的应用。分析认为:利用射频识别技术自动读取钢管管号,可以解决MES人工输入存在的输入错误、滞后等问题;射频识别设备具有远距离快速识别标签的特点,应用在钢管生产流程中可以最大限度地提高生产效率和准确性。射频识别技术应用近5年来,虽存在重复发卡、数据读取错误等问题,但通过技术和工艺调整,目前使用效果良好。     

An Advanced Analysis of Cloud Computing Concepts Based on the Computer Science Ontology

Our primary research hypothesis stands on a simple idea: The evolution of top-rated publications on a particular theme depends heavily on the progress and maturity of related topics. And this even when there are no clear relations or some concepts appear to cease to exist and leave place for newer ones starting many years ago. We implemented our model based on Computer Science Ontology (CSO) and analyzed 44 years of publications. Then we derived the most important concepts related to Cloud Computing (CC) from the scientific collection offered by Clarivate Analytics. Our methodology includes data extraction using advanced web crawling techniques, data preparation, statistical data analysis, and graphical representations. We obtained related concepts after aggregating the scores using the Jaccard coefficient and CSO Ontology. Our article reveals the contribution of Cloud Computing topics in research papers in leading scientific journals and the relationships between the field of Cloud Computing and the interdependent subdivisions identified in the broader framework of Computer Science.     

Advancing responses to climate change through improved interplay between planning theory and practice

ABSTRACT

Many planning agencies worldwide now see climate change response as unavoidable. This paper proposes that a central task for contemporary planning theory is to guide planning practice as it develops multi-dimensional responses. We examine three theoretical constructs: anticipatory governance, legitimacy and social-ecological resilience. We argue that each conceptualises challenges climate change presents to planning practice, while providing theoretically informed options for responses. Building on this, we utilize Friedmann’s [2008. “The Uses of Planning Theory: A Bibliographic Essay.” Journal of Planning Education and Research 28 (2): 247–257. doi:10.1177/0739456X08325220] tasks for planning theory as a framework to assess the utility of planning theories to guide climate change response through practice. Associated issues are discussed, including the influence of translatable planning theories and the value of importing knowledge from other disciplines. The paper concludes that more sophisticated interplay between planning theory and practice may improve planning responses to the climate change threat. The need for planning theory to translate its conceptual discoveries to the domain of practice is key.     

鄂尔多斯盆地地层水的配伍性研究

通过对中国图书馆分类法和中国科学院图书馆图书分类法及测绘学叙词表等相关资料的分析,确定本体中的类和定义类的语义关系,基于Protégé工具构建测绘学科信息公共本体、测绘学科分类—中图法本体及测绘学科分类—科图法本体,在构建的本体基础上基于Oracle 11g语义技术构建测绘学科分类本体库,为进一步语义检索提供基础.     

基于本体的改模方案聚类研究

在分析改模知识的基础上,提出了一种基于本体的聚类分析法来获得典型的改模方案,建立了注塑模改模知识领域本体,确立了用矩阵方法表达改模方案数据的数学模型。通过基于改模知识领域本体之间概念相似度的计算获得改模概念间的语义相似度、用归一化公式计算改模方案间的相似度和簇间相似度,用相似度矩阵表示改模方案间的相似度,并应用凝聚的层次聚类法进行改模方案的聚类,通过改模方案聚类粒度的确定方法获得聚类结果。以企业的改模方案数据为例,验证了该方法的有效性。