全IP电视直播节目的即时回看技术

介绍了已运用于实践中的一种全IP电视直播节目即时回看技术,用户通过遥控器一键实现直播节目的即时回看,极大地提升了用户的观看体验。通过在临汾市智慧广电全IP融合业务网建设实践项目中的使用,技术的先进性和有效性得到实践验证。     

Analysis of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay: A Comparative Study of Four Isolation Kits

There is a lack of reliable biomarkers for disorders of the central nervous system (CNS), and diagnostics still heavily rely on symptoms that are both subjective and difficult to quantify. The cerebrospinal fluid (CSF) is a promising source of biomarkers due to its close connection to the CNS. Extracellular vesicles are actively secreted by cells, and proteomic analysis of CSF extracellular vesicles (EVs) and their molecular composition likely reflects changes in the CNS to a higher extent compared with total CSF, especially in the case of neuroinflammation, which could increase blood–brain barrier permeability and cause an influx of plasma proteins into the CSF. We used proximity extension assay for proteomic analysis due to its high sensitivity. We believe that this methodology could be useful for de novo biomarker discovery for several CNS diseases. We compared four commercially available kits for EV isolation: MagCapture and ExoIntact (based on magnetic beads), EVSecond L70 (size-exclusion chromatography), and exoEasy (membrane affinity). The isolated EVs were characterized by nanoparticle tracking analysis, ELISA (CD63, CD81 and albumin), and proximity extension assay (PEA) using two different panels, each consisting of 92 markers. The exoEasy samples did not pass the built-in quality controls and were excluded from downstream analysis. The number of detectable proteins in the ExoIntact samples was considerably higher (~150% for the cardiovascular III panel and ~320% for the cell regulation panel) compared with other groups. ExoIntact also showed the highest intersample correlation with an average Pearson’s correlation coefficient of 0.991 compared with 0.985 and 0.927 for MagCapture and EVSecond, respectively. The median coefficient of variation was 5%, 8%, and 22% for ExoIntact, MagCapture, and EVSecond, respectively. Comparing total CSF and ExoIntact samples revealed 70 differentially expressed proteins in the cardiovascular III panel and 17 in the cell regulation panel. To our knowledge, this is the first time that CSF EVs were analyzed by PEA. In conclusion, analysis of CSF EVs by PEA is feasible, and different isolation kits give distinct results, with ExoIntact showing the highest number of identified proteins with the lowest variability.     

Weak Delocalization in Graphene on a Ferromagnetic Insulating Film

Graphene has been predicted to develop a magnetic moment by proximity effect when placed on a ferromagnetic film, a promise that could open exciting possibilities in the fields of spintronics and magnetic data recording. In this work, the interplay between the magnetoresistance of graphene and the magnetization of an underlying ferromagnetic insulating film is studied in detail. A clear correlation between both magnitudes is observed but through a careful modeling of the magnetization and the weak localization measurements, that such correspondence can be explained by the effects of the magnetic stray fields arising from the ferromagnetic insulator is found. The results emphasize the complexity arising at the interface between magnetic and 2D materials.     

Photo-crosslinking: An Emerging Chemical Tool for Investigating Molecular Networks in Live Cells

Studying protein–protein interactions (PPIs) is useful for understanding cellular functions and mechanisms. Evaluating these PPIs under conditions as similar as possible to native conditions can be achieved using photo-crosslinking methods because of their on-demand ability to generate reactive species in situ by irradiation with UV light. Various fusion tag, metabolic incorporation, and amber codon suppression approaches using various crosslinkers containing aryl azide, benzophenone, and diazirines have been applied in live cells. Mass spectrometry and immunological techniques are used to identify crosslinked proteins based on their capture transient and context-dependent interactions. Herein we discuss various incorporation methods and crosslinkers that have been used for interactome mapping in live cells.     

工程量清单招投标中成本界定方法之研究

介绍了工程量清单招投标中采用“经评审最低价中标法”的一些做法和特点,认为当前招投标中的“拦路虎”是成本界定问题。通过目前常见几种界定方法之比较,提出清单报价综合评判法是成本界定行之有效的方法,并提出成本界定具体做法以及应注意的问题。     

工程量清单计价模式下施工企业的成本管理

分析了新旧两种工程造价管理模式的区别所在 ,结合对新的造价管理模式内涵的理解 ,从概念分析 ,企业成本管理的变化及思路等方面 ,提出了顺利完成企业成本管理模式转变的措施     

浅谈工程预结算的重要性

介绍了预算、结算的编制内容及编制要求，表明了预结算工作的重要性，阐述了预结算资料的汇总整理方法，以正确反映工程实际造价情况，提高工程项目的利润。     

浅谈推行工程量清单招标的运行环境

从工程造价、工程经济以及招投标管理等方面对推行工程量清单招标的运行环境进行论述 ,指出只有逐步建立和完善各种配套制度 ,才能为工程量清单招标的全面推行创造条件     

2000年以来发布的有关暖通空调专业的部分国家标准和行业标准

列出了2000年以来发布的部分供暖供热、空调制冷、节能、通风除尘、洁净技术、计算机及网络技术、防火排烟、给水排水、减振降噪、环境保护、室内空气品质、材料与设备等标准．以及有关设计、验收规范和一些综合类标准。     

浅谈工程量清单计价

结合工程量清单计价与定额计价法的概念 ,对此两种计价方式的区别作了分析 ,介绍了工程量清单计价这种新型计价方式的主导原则 ,指出工程量清单计价对完善工程造价改革起到了积极的作用。