Image sequence-based risk behavior detection of power operation inspection personnel

A novel image sequence-based risk behavior detection method to achieve high-precision risk behavior detection for power maintenance personnel is proposed in this paper. In this method, the original image sequence data is first separated from the foreground and background. Then, the free anchor frame detection method is used in the foreground image to detect the personnel and correct their direction. Finally, human posture nodes are extracted from each frame of the image sequence, which are then used to identify the abnormal behavior of the human. Simulation experiment results demonstrate that the proposed algorithm has significant advantages in terms of the accuracy of human posture node detection and risk behavior identification.     

基于改进残差特征蒸馏的轻量级超分辨率网络

基于深度学习的图像超分辨率算法通常采用递归的方式或参数共享的策略来减少网络参数，这将增加网络的深度，使得运行网络花费大量的时间，从而很难将模型部署到现实生活中。为了解决上述问题，本文设计一种轻量级超分辨率网络，对中间特征的关联性及重要性进行学习，且在重建部分结合高分辨率图像的特征信息。首先，引入层间注意力模块，通过考虑层与层之间的相关性，自适应地分配重要层次特征的权重。其次，使用增强重建模块提取高分辨率图像中更精细的特征信息，以此得到更加清晰的重建图片。通过大量的对比实验表明，本文设计的网络与其他轻量级模型相比，有更小的网络参数量，并且在重建精度和视觉效果上都有一定的提升。     

Cloning,sequencing and structural analysis of membrane-bound polyphenol oxidase from Granny Smith apples (Malus × domestica Borkh)

The gene sequence coding for the membrane-bound polyphenol oxidase (mPPO) with a length of 1761 bp was cloned by PCR method and shown to contain one highly conserved sequence encoding a di-copper-binding region. The predicted three-dimensional structure of mPPO indicated that the active site was located near two copper ions and composed of a typical bundle of four α-helices. Each of the two catalytic copper ions was coordinated with three histidine residues in the hydrophobic pocket, yielding His 180, His 201, His 210, His 332, His 336 and His 366. Docking studies showed that 4-methylcatechol and chlorogenic acid have different binding models due to different ligand sizes and binding sites in the active centre, and it was found that the smaller compound exhibited a higher affinity for mPPO. Molecular dynamic simulation results indicated that Phe 353 is important in controlling enzymatic activity through influencing substrate coordination in the active site.     

超高效液相色谱⁃四极杆⁃飞行时间质谱对含PET食品接触材料中可迁移非挥发性物质的筛查研究

采用超高效液相色谱⁃四极杆⁃飞行时间高分辨质谱（UPLC⁃Q⁃TOF）对4类不同类型的含聚对苯二甲酸乙二醇酯（PET）材质的食品接触材料在4 %乙酸和50 %乙醇模拟物中的迁移出的非挥发性未知物进行筛查解析。结果表明，产品在4 %乙酸模拟物的迁移风险远小于50 %乙醇模拟物，主要迁移物质为聚合单体形成的寡聚物，抗氧剂、润滑剂、胶黏剂等加工助剂以及生产加工、迁移过程中形成的非有意添加物（NIAS）物质；纯PET材质的产品迁移物质较少，多层复合材料迁移物质较多。复合材质的产品中，PET材质可能在生产时添加了己二酸、癸二酸、新戊二醇等物质，进行了改性处理；此外，部分迁移物质会与模拟物中的乙醇发生反应，生成新的NIAS物质。     

Lactones in the Synthesis of Prostaglandins and Prostaglandin Analogs

In the total stereo-controlled synthesis of natural prostaglandins (PGs) and their structural analogs, a vast class of compounds and drugs, known as the lactones, are encountered in a few key steps to build the final molecule, as: δ-lactones, γ-lactones, and 1,9-, 1,11-, and 1,15-macrolactones. After the synthesis of 1,9-PGF_2α and 1,15-PGF_2α lactones, many 1,15-lactones of E₂, E₃, F₂, F₃, A₂, and A₃ were found in the marine mollusc Tethys fimbria and the quest for understanding their biological role stimulated the research on their synthesis. Then 1,9-, 1,11-, and 1,15-PG lactones of the drugs were synthesized as an alternative to the corresponding esters, and the first part of the paper describes the methods used for their synthesis. The efficient Corey procedure for the synthesis of prostaglandins uses the key δ-lactone and γ-lactone intermediates with three or four stereocenters on the cyclopentane fragment to link the PG side chains. The paper describes the most used procedures for the synthesis of the milestone δ-Corey-lactones and γ-Corey-lactones, their improvements, and some new promising methods, such as interesting, new stereo-controlled and catalyzed enantioselective reactions, and methods based on the chemical/enzymatic resolution of the compounds in different steps of the sequences. The many uses of δ-lactones not only for the synthesis of γ-lactones, but also for obtaining 9β-halogen-PGs and halogen-substituted cyclopentane intermediates, as synthons for new 9β-PG analogs and future applications, are also discussed.     

Q值正演模拟约束下的零偏移距VSP资料Q值估算

随着油气勘探目的层逐步走向深部,球面扩散、透射损失、环境噪声等使得VSP资料的初至子波发生改变,导致常规的Q值估算方法质量难以得到保证。为此,利用塔里木盆地托甫台地区TP327井零偏移距VSP资料,综合考虑透射损失和球面扩散的影响,提出一种Q值正演模拟约束下的零偏移距VSP资料Q值估算方法。该方法通过研究区的纵波速度、横波速度和密度建立层状地质模型,利用射线追踪的方法,用大地吸收介质模型的Q值正演模拟结果,对频谱比法和经验公式估算的初始Q值进行修正,使得Q值正演模拟结果与实际地震波的衰减记录在频谱特征上吻合,从而得到更为精确的Q模型。TP327井Q值估算结果表明,该方法与李庆忠经验公式得到的结果在浅层比较接近,但在中层和深层,两者差异较大;Q值正演模拟结果约束的子波与实际VSP资料子波的频谱比较吻合,证实用Q值正演模拟约束得到的层间Q值更为可靠。利用Q值正演模拟约束方法得到的Q模型对研究区地震资料进行反Q滤波能显著提高地震资料分辨率,有助于提高碳酸盐岩地层中缝洞的识别精度。     

Compilation of Modern Technologies To Map Genome-Wide Cytosine Modifications in DNA

Over the past few decades, various DNA modification detection methods have been developed; many of the high-resolution methods are based on bisulfite treatment, which leads to DNA degradation, to a degree. Thus, novel bisulfite-free approaches have been developed in recent years and shown to be useful for epigenome analysis in otherwise difficult-to-handle, but important, DNA samples, such as hmC-seal and hmC-CATCH. Herein, an overview of advances in the development of epigenome sequencing methods for these important DNA modifications is provided.     

Viscosity reduction of acidic heavy oil through emulsification: effects of salinity and pH

In this research, sodium dodecyl sulfonate (SDS) was selected as emulsifying viscosity reducer through a great number of laboratory experiments and then the effects of salinity and pH on viscosity reduction effects were evaluated. The result showed that higher salinity would result in emulsion viscosity enhancement. Compared with neutral pH conditions (pH =7), both acidic and basic conditions would promote emulsion viscosity reduction. When pH >7, generated O/W emulsions were much more stable than all other emulsions. Detailed compositional characterization of petroleum acid through a high resolution mass spectrometer (FT-ICR MS) proved that carboxylic acid and phenols inside petroleum acid would react with OH^- at basic conditions and produce some natural surfactants. Theses natural surfactants could work synergistically with SDS and further improve emulsion stability.     

基于食品组学技术的干邑白兰地真假鉴别研究

目的建立一种基于食品组学技术对干邑白兰地真假鉴别的方法。方法白兰地样品过0.45μm滤膜,在高分辨质谱Full MS/dd MS2扫描模式下采集数据,采用Compound Discoverer 3.0对样品数据进行主成分分析(principal component analysis, PCA)和差异分析,并通过mzCloud网络数据对干邑白兰地中的化合物检索鉴定。结果 PCA分析显示真假白兰地和同一品牌不同等级的白兰地均可以实现明显区分。根据差异化合物的母离子的精确质量数、母离子同位素组成及二级质谱信息鉴定出19种化合物,这些化合物在真假酒样中含量不同。结论该方法简单、快速、准确,为干邑白兰地的真假鉴别及组分鉴定提供参考。     

Effect of ZnO/K2O ratio on the crystallization sequence and microstructure of lithium disilicate glass-ceramics

The effect of ZnO/K₂O (Z/K) ratio on the crystallization sequence and microstructure of lithium disilicate (Li₂Si₂O₅: LS2) glass-ceramics was carefully investigated for the SiO₂-Li₂O-K₂O-ZnO-P₂O₅ system. The Z/K ratios of precursor glasses were varied from 0 to 3.5 while the nucleating agent of P₂O₅ and glass modifiers of ZnO plus K₂O were fixed to have 1.5 and 4.5 mol% relative to LS2, respectively. For the samples prepared by two-stage heat treatments of 500 °C for 1 h and 800 °C for 2 h in air, the LS2 nucleation rate was increased with increasing the Z/K ratio due to the variation in crystallization sequence from type II (Li₂SiO₃: LS) to type I (LS + LS2) in addition to an amorphous phase separation in base glass. Consequently, with increasing the Z/K ratio, the LS2 crystalline phase within the glass matrix continuously changed from larger acicular ones to smaller equiaxed ones.