Sulfate- and pH-driven metabolic flexibility in sugarcane vinasse dark fermentation stimulates biohydrogen evolution,sulfidogenesis or homoacetogenesis

Dark fermentation of sugarcane vinasse can be used as a “cleaning” step to remove sulfate prior to methanogenesis because sulfidogenic conditions can be successfully established in parallel with biohydrogen production. Using a 2² central composite rotational design (CCRD) and response surface methodology (RSM), this study assessed the impacts of bicarbonate and sulfate availability on the establishment of sulfidogenesis in the thermophilic (55 °C) fermentation of vinasse in batch reactors, equally assessing the impacts on biohydrogen evolution. CCRD-RSM results indicated the favoring of biohydrogen production at the lowest sulfate and bicarbonate concentrations, whilst the opposite was observed for sulfidogenesis. Glycerol, lactate, and hydrogen were the preferential electron donors utilized by sulfate-reducing bacteria (SRB), whilst ethanol was markedly consumed only at high sulfate concentrations. SRB were inhibited by sodium when dosing excess NaHCO₃ and Na₂SO₄. Complementary tests revealed maximum biohydrogen production (2.40 mmol) out of the CCRD, at pH exceeding 7.5 with no interference of sulfidogenesis. Non-efficient biohydrogen production was observed at low pH (<5.0; ～1.90 mmol) because the uptake of lactate was inhibited. Meanwhile, homoacetogenesis was established under intermediate pH range (5.5–6.5), as revealed by the accumulation of acetate (up to 2.5 g L^?1). 16S rRNA gene amplicon sequencing further revealed the genera Thermoanaerobacterium/Pseudoclostridium, Desulfotomaculum/Desulfohalotomaculum and Sporomusaceae/Moorella as the main biohydrogen-producing, sulfate-removing and biohydrogen-consuming (homoacetogens) microbial groups, respectively. Hence, using a single inoculum source, vinasse may provide a butyrate-rich (along with biohydrogen-rich biogas) or a sulfate-free and acetate-rich fermented effluent, depending mainly on proper pH control.     

毫米波三维异质异构集成技术的进展与应用

三维异质异构集成技术是实现电子信息系统向着微型化、高效能、高整合、低功耗及低成本方向发展的最重要方法,也是决定信息化平台中微电子和微纳系统领域未来发展的一项核心高技术。文章详细介绍了毫米波频段三维异质异构集成技术的优势、近年来的发展趋势以及面临的挑战。利用硅基MEMS 光敏复合薄膜多层布线工艺可实现异质芯片的低损耗互连,同时三维集成高性能封装滤波器、高辐射效率封装天线等无源元件,还能很好地处理布线间的电磁兼容和芯片间的屏蔽问题。最后介绍了一款新型毫米波三维异质异构集成雷达及其在远距离生命体征探测方面的应用。     

Single Cell Bioprinting with Ultrashort Laser Pulses

Tissue engineering requires the precise positioning of mammalian cells and biomaterials on substrate surfaces or in preprocessed scaffolds. Although the development of 2D and 3D bioprinting technologies has made substantial progress in recent years, precise, cell-friendly, easy to use, and fast technologies for selecting and positioning mammalian cells with single cell precision are still in need. A new laser-based bioprinting approach is therefore presented, which allows the selection of individual cells from complex cell mixtures based on morphology or fluorescence and their transfer onto a 2D target substrate or a preprocessed 3D scaffold with single cell precision and high cell viability (93–99% cell survival, depending on cell type and substrate). In addition to precise cell positioning, this approach can also be used for the generation of 3D structures by transferring and depositing multiple hydrogel droplets. By further automating and combining this approach with other 3D printing technologies, such as two-photon stereolithography, it has a high potential of becoming a fast and versatile technology for the 2D and 3D bioprinting of mammalian cells with single cell resolution.     

Implication of Size-Controlled Graphite Nanosheets as Building Blocks for Thermal Conductive Three-Dimensional Framework Architecture of Nanocarbons

Preparation of three-dimensional (3D) networks has received significant attention as an effective approach for applications involving transport phenomena, such as thermal management materials, and several nanomaterials have been examined as potential building blocks of 3D networks for the improvement of heat conduction in polymer nanocomposites. For that purpose, nanocarbons such as graphene and graphite nanoplatelets have been spotlighted as suitable filler materials because of their excellent thermal conductivities (ca. 10²–10³ W·(m·K)^?1 along their lateral axes) and morphological merits. However, the implications of morphological features such as the lateral length and thickness of graphene or graphene-like materials have not yet been identified. In this study, a controlled dissociation of bulk graphite to graphite nanosheets (GNSs) using a low-cost, ecofriendly bead mill process was extensively examined and, when configured in a 3D framework architecture formation, the size-controlled GNSs demonstrated that the thermal conductivities of a 3D interconnected framework of GNSs and the corresponding polymer nanocomposite were intimately correlated with the size of the GNSs, thus demonstrating the successful preparation of an efficient thermal management material without highly sophisticated efforts. The capability of controlling the lateral size and thickness of the GNSs as well as the use of a 3D interconnected framework architecture should greatly assist the commercialization of high-quality graphene-based thermal management materials in a scalable production process.     

基于数字单元法的三维正交织物微观几何结构建模

为了反映织物内部纱线的空间构型和微观几何结构,针对在织物建模过程中,因忽略纤维间的相互作用而引起的纱线截面形状变化的问题,基于数字单元法理论,提出了一种计算纤维间摩擦力的方法。通过纱线纤维化离散,用数值模拟和仿真方法模拟三维正交织物成型过程,建立了5种精度递进的微观几何结构数值模型。5种模型中的每根纱线分别由4、7、12、19和37根数字纤维表征。研究结果表明:随着纱线纤维化离散程度的提高,仿真时间延长,织物厚度减小,纤维体积分数增大,节点平均作用力下降速度变缓,势能变小;当每根纱线由19根数字纤维组成时,所建织物的微观几何结构数值模型与真实织物样本在显微镜下的内部切片图像较为吻合。     

抽水蓄能电站施工现场安全文明施工三维策划应用研究

基于电网安全对稳定经济运行的重要作用,国家大力推进抽水蓄能电站的建设,"十三五"期间,将进入抽水蓄能电站建设高峰期,抽水蓄能电站施工现场点多面广,基建安全形势严峻,施工现场的安全是工程管理的核心因素[1].为了提升现场安全文明施工管理水平,其核心在于施工现场安全文明施工策划,策划的水平和可操作性决定了建筑施工过程中的安全管理的成效,而行之有效的策划应用模式成为关键.本文结合山东文登抽水蓄能电站安全文明施工三维策划管理及三维策划系统应用,解决了现有安全文明施工策划、现场安全布置、安全设施标准化等环节的局限性及不足,实现了策划的高效快捷、效果直观真切,探索出了安全文明施工策划应用的新途径.     

一种重载汽车轮毂总成自动点焊机器人的研制

针对手工焊接汽车轮毂总成效率低下、焊接质量不稳定等问题及一种重载汽车轮毂总成的结构特点及焊接要求，设计了一种直角坐标型自动点焊机器人。阐述了自动点焊机器人本体的方案设计、控制系统应用等过程，并利用现场试验与工业性试验对自动点焊机器人的性能等进行了验证，为重载汽车轮毂总成的自动焊接提供了一种可供借鉴的方法。      

基于三维投影矩阵的生产流水线对接系统平台构建

针对工作台扭摆会导致机械加工对接产生误差,提出基于三维投影矩阵构建生产流水线对接系统平台,以降低对接误差。三维运动平台采用脉冲信号控制的步进电机进行驱动,达到机床限位控制;通过控制激光器开关和控制激光器的输出功率实现系统的激光控制;通过多普勒频差测量位移技术,采用交流前置放大器替换常规直流放大器等,获取精准实时测量数据;根据三维立体视觉校对原理提取对接区域图像特征点,依据特征点校验机械加工平台三维对接图像时,采用Powell方法求解三维阶段投影矩阵对接目标函数最小值,实现机械加工的高精准度对接。结果表明:机械加工对接的最大相对误差为1.50%,精准度高,进行机械加工耗时18～23min,花费时间较短。     

基于UG的采煤机防爆盖板三维标注及数控工艺研究

随着三维CAD技术的发展,产品制造信息在三维模型和二维图纸之间的反复传递,严重影响了产品制造信息的正确、快速传递。基于三维产品制造信息技术,以采煤机防爆盖板为例,在UG NX10.0中建立了防爆盖板的三维模型并进行了三维标注,设计了加工工艺路线,规划了数控加工刀具轨迹,进行了加工仿真,验证了数控加工的正确性,在此基础上完成了采煤机防爆盖板的三维工艺式设计。该研究对基于模型定义的数字化设计与制造研究,实现制造业信息化具有重要意义。     

基于改进蚁群算法的三维路径规划

针对移动机器人在海水环境中的三维路径规划问题容易陷入局部最优和收敛慢等瑕疵，根据三维环境全局信息来改进蚁群算法以提高实时性和收敛速度。改进蚁群算法的启发函数，采用局部信息和全局信息结合动态地改进信息素更新方式，以及根据三维空间中路径的平坦程度和光滑度二阶微分分别增加了一阶微分和二阶微分来再次修改信息素更新规则。仿真对比实验结果显示改进后的蚁群算法克服了收敛速度慢、容易陷入局部最优的缺点。