Review of free software tools for image analysis of fluorescence cell micrographs

An increasing number of free software tools have been made available for the evaluation of fluorescence cell micrographs. The main users are biologists and related life scientists with no or little knowledge of image processing. In this review, we give an overview of available tools and guidelines about which tools the users should use to segment fluorescence micrographs. We selected 15 free tools and divided them into stand‐alone, Matlab‐based, ImageJ‐based, free demo versions of commercial tools and data sharing tools. The review consists of two parts: First, we developed a criteria catalogue and rated the tools regarding structural requirements, functionality (flexibility, segmentation and image processing filters) and usability (documentation, data management, usability and visualization). Second, we performed an image processing case study with four representative fluorescence micrograph segmentation tasks with figure‐ground and cell separation. The tools display a wide range of functionality and usability. In the image processing case study, we were able to perform figure‐ground separation in all micrographs using mainly thresholding. Cell separation was not possible with most of the tools, because cell separation methods are provided only by a subset of the tools and are difficult to parametrize and to use. Most important is that the usability matches the functionality of a tool. To be usable, specialized tools with less functionality need to fulfill less usability criteria, whereas multipurpose tools need a well‐structured menu and intuitive graphical user interface.     

基于老年人偏好的手机图标尺寸可用性设计研究

目的提高老年人对手机界面的用户体验。方法以老年人手机界面中的启动图标为研究对象,根据Android系统中手机界面图标的设计规范,围绕48×48 px,72×72 px,96×96 px 3种常见图标尺寸,采用单因素实验方法对手机图标的可用性进行研究。结论手机图标的设计应满足用户的使用习惯,并应兼顾界面的整体美感,只有这样才能具备良好的可用性。     

Shifting the Design Philosophy of Spoken Natural Language Dialogue: From Invisible to Transparent Systems

Spoken natural language interfaces are characterized by a high degree of opacity. This characteristic leads many new users to converse with the machine on the basis of an inaccurate image of the system, which results in numerous dysfunctions and failures in the human-machine spoken dialogue. The application of the principle of transparency aims to correct this problem by enabling the system to make it “visible” (it can, so to speak, be seen “from the inside”). This report presents a study that compares two versions of the same spoken dialogue system that differ only by some of their dialogue strategies. In particular, one version of this system starts the dialogue with a recall of the main system's function followed by an open-ended prompt, while the other version only produces an open-ended prompt (What can I do for you?). Moreover, one version of this system makes explicit some possible actions in specific dialogue contexts, while the other version leaves these possible actions implicit. These strategies were compared by having a group of 28 users test them in real conditions of use. The results highlight that transparency strategies can help users in formulating simple and acceptable requests, satisfying their goals more easily while provoking less recognition and comprehension errors.     

Measuring website quality: asymmetric effect of user satisfaction

Website quality measurement tools have been largely static and have struggled to determine relevant attributes of user satisfaction. This study compares and contrasts attributes of user satisfaction based on usability guidelines seeking to identify practical easy-to-administer measurement tools. The website users assessed business school homepages according to six criteria and fulfilled a randomly assigned yet typical task. After completing the task, respondents assessed the same six website quality/satisfaction criteria again. The consumer–product relationship seems similar to the link between a user and a website. User satisfaction, just like consumer satisfaction, is asymmetric and non-linear. Content and navigation have been identified as key ingredients when users judged website quality, alerting web designers and website practitioners to focus more closely on those attributes. Similar lessons can be drawn for marketing professionals, who typically supervise or determine the content, structure and other website facets.     

浅谈可用性的GUI设计

GUI设计已成为软件设计中最为重要的部分之一。本文阐述了GUI在设计上的发展及其趋势,并以提升可用性为目的对GUI设计的基本要素进行了分析。     

The importance of task analysis in Usability Context Analysis - designing for fitness for purpose

Usability Context Analysis (UCA) suggests the use of task analysis in order to characterize the user's requirements of a product. This paper shows that a task analysis is a necessary (but not sufficient) part of a usability context analysis. Further, it is argued that it is necessary to carry out the task analysis to sufficient depth to establish fitness for purpose of the product under test. In addition, the analyst should have some knowledge of the application domain so that from various task sequences, that giving the best user-product task match can be used. The paper indicates by using an example of an echosounder that discrepancies of use can be highlighted through this task-based approach to usability context analysis.