Rosenbrock算法在分析化学中的应用

本文介绍了Rosenbrock算法在分析化学中的若干应用。分别讨论了数学模型,并报告了应用实例。     

牛血清白蛋白三元体系结合反应的研究

利用牛血清白蛋白(BSA)对稀土铽离子(Tb3+)的荧光敏化增强作用即以Tb3+为荧光探针,分别研究了柠檬酸(Cit),草酸(H2C2O4)和蛋氨酸(Met)与Tb3+、BSA三元体系的络合反应情况。结果表明:Cit,H2C2O4均与Tb3+、BSA生成了三元配合物,而Met则未发生络合反应。     

三维荧光结合交替三线性方法测定中药升麻和人体血浆中升麻素的含量

本文利用化学计量学交替三线性分解(ATLD)方法解析三维荧光数据,在有未知干扰组分共存的情况下,对中药升麻和人体血浆中升麻素的含量和回收率进行了测定,利用核一致诊断法判断得到的组分数是2。中药升麻和血浆体系的背景与升麻素有严重的重叠,用ATLD方法得到的解析光谱与真实光谱几乎完全重合,定量解析结果也令人满意。测定结果显示,标准曲线法和二阶标准加入法得到的中药升麻中升麻素的含量分别为0.061±0.003%和0.055±0.002%,回收率为89.8±4.7%,人体血浆中升麻素的回收率分别为102.7±1.6%。实验结果表明,此法可用于干扰组分共存下中药升麻和人体血浆中升麻素的快速准确定量分析。     

光度法测定有机试剂逐级电离常数的数学理论与计算方法

本文研究了光度法测定有机试剂逐级电离常数的理论模型的数学性质，提出了处理实验数据的一种快速算法，从理论上分析了计算方法的收敛性，并给出了应用实例。     

Determination of Composition and Stability Constant of Praseodymium(Pr~(3+))Complex with Tribromoarsenazo(TBA)by Dual-Series Linear Regression Method

A new method,dual-series linear regression method,has been used to study the complexationequilibrium of praseodymium(Pr~(3+))with tribromoarsenazo(TBA)without knowing the accurate concentra-tion of the complexing agent TBA.In 1.2 mol/L HCl solution, Pr~(3+)reacts with TBA and forms 1:3 com-plex,the conditional stability constant(lgβ_3)of the complex determined is 15.47,and its molarabsorptivity(ε_3~(630))is 1.48×10~5 L·mol~(-1)·cm~(-1).     

Determination of Compositions and Stability Constants of Holmium and Yttrium Complexes with Tribromoarsenazo by Linear Regression Method

According to the appearing of isosbestic point in the absorption spectra of Ho/Y-Tribromoarsenazo(TBA)systems,the complexation reaction is considered to be M nL=ML_n.A method has been proposed basedon it for calculating the mole fraction of free complexing agent in the solutions from spectral data.and two linearregression formula have been introduced to determine the composition,the molar absorptivity,the conditionalstability constant of the complex and the concentration of the complexing agent.This method has been used in Ho-TBA and Y-TBA systems.Ho~(3 )and Y~(3 )react with TBA and form 1:2 complexes in HCl-NaAc buffer solution at pH 3.80.Their molar absorptivities determined are 1.03×10~8 and1.10×10~8 cm~2·mol~(-1),and the conditional stability constants(logβ_2)are 11.37 and 11.15 respectively.Afterconsidering the pH effect in TBA complexing,their stability constants(log β_2~(ahs))are 43.23 and 43.01.respectively.The new method is adaptable to such systems where the accurate concentration of the complexingagent can not be known conveniently.     

牛血清白蛋白三元体系结合反应的研究

利用牛血清白蛋白（BSA）对稀土铽离子（Tb3＋）的荧光敏化增强作用即以Tb3＋为荧光探针,分别研究了柠檬酸（Cit）,草酸（H2C2O4）和蛋氨酸（Met）与Tb3＋、BSA三元体系的络合反应情况.结果表明：Cit,H2C2O4均与Tb3＋、BSA生成了三元配合物,而Met则未发生络合反应.     

Rosenbrock算法在三溴偶氮胂逐级电离常数测定中的应用

推导了光度法测定有机试剂的逐级电离常数及各型体的摩尔吸光系数的计算通式。选用Rosenbrock 算法处理实验数据。在20℃,离子强度 I=0.4的条件下,测得三溴偶氮胂的六级电离常数(pk_2)分别为:0.60、1.56、2.81、6.59、9.50和11.26。