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The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and growth rates were set at (0.15, 0.2, 0.25h-1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase spectively. The model predictions are in good agreement with the experimental data. 相似文献
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漆树漆汁道的超微结构研究 总被引:2,自引:0,他引:2
<正> 生漆是一种优良的天然涂料,是漆树代谢过程的次生产物,由漆汁道分泌和贮芷。近年来,国内外对于漆树及其同科植物漆汁道的显微结构方面已有许多研究报道,但对我国盛产生漆的漆树(Rhus verniciflua)漆汁道的超显微结构至今尚未见报道。为此,我们以漆树树皮为材料,对其次生韧皮部中漆汁道的超微结构进行了观察,并进一步探讨生漆的形成与漆汁道细胞超微结构的关系。现将研究结果报道于后。 相似文献
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漆树花器官中漆汁道的分布和发育 总被引:2,自引:0,他引:2
本文报道了漆树花器官中漆汁道的发生和发育方式。研究结果表明,萼片、花瓣和子房中漆汁道是以裂生方式产生的。但发育到一定阶段,漆汁道腔周围的分泌细胞有与漆汁道中分泌物相似的染色反应。随后,这些细胞脱入腔道,逐渐解体。与此同时,暴露在腔道周围的薄壁细胞逐渐变成分泌细胞。因此,在漆树花器官(雄蕊除外)中,漆汁道的发育是裂溶生的。 相似文献
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The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and Yx/s were determined as 1.75h, 0.65h^-1 and 0.51g·g^-1, respectively. In the fed-batch culture, different specific growth rates were set at (0.15, 0.2, 0.25h^-1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase were obtained. The result shows that the concentrations of cell and product can reach 77.5g·L^-1 and 10.2g·L^-1 respectively. The modal predictions are in good agreement with the experimental data. 相似文献
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漆树乳汁道的结构规律及其应用 总被引:3,自引:3,他引:0
生漆是一种优良的天然涂料,由漆树的乳汁道分泌和贮存。本文研究了漆树乳汁道的显微和超微结构,分布和发育,并将乳斗道的结构规律应用于改进割漆技术的选育漆树优良品种。在漆树中,乳汁道是一种裂生的分泌道,其分泌细胞的外面又有2-3层鞘细胞所围绕,它们主要分布在漆树各个器官的韧皮部中。根据对漆对乳汁道发育不同时期分泌细胞的电子显微镜观察,发现在乳汁道分泌细胞中含有大量的质体、内质网和嗜饿物质。在乳汁道发育过 相似文献