Morphological changes induced by colchicine in the chick optic cup in early stages of development. A stereological study

In this study chick embryo optic cups at HH stage 13 of development were analyzed under normal conditions and after inoculation with colchicine for 1, 2, 4, and 8 h. Several changes were seen after these periods of treatment: 1) modifications of the structure, with thicker regions in the cup and a general decrease in the total volume according to the duration of exposure to the drug (about 4 times less than normal, 5,035 x 10(3) microns 3 vs 1,334 x 10(3) microns 3 after 8 h of treatment); 2) enlargement of the ventricular cavity and its closure, due to failure of approximation of retinal and pigmentary layers; 3) failure of lens development, with delay and impairment of pit formation and deformation of all structures; lens volume was less than normal (about 4 times less, 2,148 x 10(3) microns 3 vs 658 x 10(3) microns 3 after 8 h of treatment); 4) a general segregation of the cells making up the structure, principally in the more active proliferating zones. The local alterations found are described.     

Syndecan-1 Depletion Has a Differential Impact on Hyaluronic Acid Metabolism and Tumor Cell Behavior in Luminal and Triple-Negative Breast Cancer Cells

Glycosaminoglycans (GAGs) and proteoglycans (PGs) are major components of the glycocalyx. The secreted GAG and CD44 ligand hyaluronic acid (HA), and the cell surface PG syndecan-1 (Sdc-1) modulate the expression and activity of cytokines, chemokines, growth factors, and adhesion molecules, acting as critical regulators of tumor cell behavior. Here, we studied the effect of Sdc-1 siRNA depletion and HA treatment on hallmark processes of cancer in breast cancer cell lines of different levels of aggressiveness. We analyzed HA synthesis, and parameters relevant to tumor progression, including the stem cell phenotype, Wnt signaling constituents, cell cycle progression and apoptosis, and angiogenic markers in luminal MCF-7 and triple-negative MDA-MB-231 cells. Sdc-1 knockdown enhanced HAS-2 synthesis and HA binding in MCF-7, but not in MDA-MB-231 cells. Sdc-1-depleted MDA-MB-231 cells showed a reduced CD24-/CD44+ population. Furthermore, Sdc-1 depletion was associated with survival signals in both cell lines, affecting cell cycle progression and apoptosis evasion. These changes were linked to the altered expression of KLF4, MSI2, and miR-10b and differential changes in Erk, Akt, and PTEN signaling. We conclude that Sdc-1 knockdown differentially affects HA metabolism in luminal and triple-negative breast cancer model cell lines and impacts the stem phenotype, cell survival, and angiogenic factors.     

基于BP神经网络的模糊PID风量控制

针对煤矿主风机通风系统多变量、非线性、时变滞后性等问题,提出一种基于BP神经网络的模糊PID算法。该算法综合神经网络、模糊控制与PID调节的各自优点,既具有神经网络的自学习和自适应能力,又具有模糊控制的非线性控制作用,同时兼备PID调节的广泛性。仿真结果表明,该算法的响应速度、稳态精度均优于传统的PID调节,取得比较理想的控制效果。     

Impact of cycling temperatures on Fusarium verticillioides and Fusarium graminearum growth and mycotoxins production in soybean

BACKGROUND: Fusarium graminearum and F. verticillioides are two very important mycotoxigenic species as they cause diverse diseases in crops. The effects of constant and cycling temperatures on growth and mycotoxin production of these species were studied on soybean based medium and on irradiated soya beans. RESULTS: F. graminearum grew better when was incubated at 15, 20 and 15–20 °C (isothermal or cycling temperature) during 21 days of incubation. Maximum levels of zearalenone and deoxynivalenol (39.25 and 1040.4 µg g^?1, respectively) were detected on soya beans after 15 days of incubation and the optimal temperature for mycotoxin production was 15 °C for zearalenone and 20 °C for deoxynivalenol. F. verticillioides grew better at 25 °C in culture medium and at 15/20 °C and 15/25 °C on soybean seeds. Fumonisin B₁ was produced only in culture medium, and the maximum level (7.38 µg g^?1) was found at 15 °C after 7 days of incubation. CONCLUSION: When growth and mycotoxin production under cycling temperatures were predicted from the results under constant conditions, observed values were different from calculated for both species and substrate medium. Therefore, care should be taken if data at constant temperature conditions are to be extrapolated to real field conditions. Copyright © 2012 Society of Chemical Industry     

Separation of Acylglycerides Obtained by Enzymatic Esterification Using Solvent Extraction

New avenues to add value to glycerol are currently being explored. One of them is the synthesis of structured lipids through glycerol esterification. In this work we have analyzed the recovery and purification of dicaprin obtained by esterification of glycerol with capric acid (C) in heptane, mediated by Lipozyme RM IM. This is an intermediate step to obtain lipids MLM. In the first stage, the diglyceride synthesis MGM (being G a central HC–OH) was carried out. When M = C, the diglyceride is CGC. Recovery of the diglyceride CGC is required to carry out the esterification of the sn-2 position with palmitic acid (P), thus obtaining the triglyceride CPC. Different solvents were evaluated using Ecofac 1.0 (a molecular design software solvent) through a theoretical approach to explore the best solvents for the acylglycerides separation. Then, the performance of the selected solvents to separate dicaprin from mono and tricaprin was experimentally studied in a liquid–liquid extraction process. Previously, the remaining fatty acid had been neutralized. With liquid–liquid extraction in three simple steps, using ethanol/water, 94 % of the dicaprin obtained by enzymatic esterification was recovered with a purity of 89 % (wt%). It was also possible to obtain dicaprin with a purity of 97 % but with a yield of 56 %.     

Framework of emergence: from chain of value to value constellation

ABSTRACT

For academic design programmes and research councils, finding mechanisms to measure impact and valuate co-design research projects is very difficult. The metric parameters – number of attendees, volume, shape, length – have shown to be insufficient. The subject matter, the subjects and matters, do not easily fit within these parameters, which fail to translate the value of a ‘constellation of affections’ that emerge throughout co-design processes. Emphasis on quantifiable impact derives from particular ways of understanding production, reproduction, and dissemination of knowledge. This paper understands academic design programmes as a technology to discuss axiology in co-design practices. This paper re-configures a techno-logic of value; from chain of value to value constellation, intersecting a co-design case study with readings of Simondon’s philosophy of technology and Barad’s agential realism. Ageing Playfully was a knowledge exchange project from The Creative Exchange programme, that involved people living with dementia and their caregivers co-designing interventions to boost mobility and well-being. At the core, this paper, explores an axiological move that informs a framework of emergence, a way of thinking in methods to account for the value of co-design as site of emergence.     

Experiments with micro-fin tube in single phase

This work shows heat transfer and friction characteristics for water single-phase flow in micro-fin tubes. The analysis of thermal and hydraulic behavior from a laminar to a turbulent flow was carried out in an experimental setup with a 9.52 mm diameter micro-fin tube. The tube was wrapped up with an electrical resistance tape to supply a constant heat flux to its surface. Different operational conditions were considered in the heating tests. The inlet and outlet temperatures, differential wall temperatures along the tube, pressure drop and flow rate were measured. The relationships of heat flux and flow rate with heat transfer coefficient and pressure drop were analyzed. Under the same conditions, comparative experiments with an internally smooth tube were conducted. The micro-fin tube provides higher heat transfer performance than the smooth tube (in turbulent flow h_micro-fin/h_smooth=2.9). In spite of the increase in pressure drop (Δp_micro-fin/Δp_smooth=1.7) the heat transfer results were significantly higher (about 80%). This shows the advantages of this enhanced configuration in thermal performance related to conventional tubes. The smooth tube results were validated by the comparison with the Dittus–Boelter and Gnielinski correlations. For the micro-fin tube an empirical correlation to the heat transfer coefficient adjusted from the set of measured data is proposed. The values obtained are in conformity with experimental results.