Special Issue on the Internet of Things (ISCC 2020)

Journal of Signal Processing Systems -     

A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-Reperfusion

Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection.     

Teaching and Learning Electrochemistry

Numerous conceptual difficulties and misconceptions have been reported in the science and chemistry education literature about electrochemistry concepts such as electrolytes, redox equations, and about electrochemical (galvanic and electrolytic) cells. Other studies have considered teaching approaches aimed at improving learning and at overcoming misconceptions. This paper reviews these studies and considers strategies and approaches for the effective teaching of electrochemistry. Then, the review focuses on problem solving in equilibrium electrochemistry, especially problems involving the Nernst equation. The main findings of four studies with Greek university chemistry students, dealing with: (i) algorithmic problem‐solving ability; (ii) practice on problem solving; (iii) the effect of the format of a problem; and (iv) the construction and evaluation of a website devoted to electrochemistry problem solving, are reported.     

AssetCollector: a system for managing collections of cultural assets

In this paper, AssetCollector is presented, which is a system for managing collections of cultural assets. AssetCollector covers the needs of collection curators towards defining, populating and searching a collection in a flexible way, while supporting them in generating reports based on the collection’s assets and reusing them in order to build web sites and CD-ROMs. In order to support the above functionality, the system provides the content structuring subsystem, the content input subsystem, the search subsystem and the report subsystem. The use of the subsystems is straightforward and requires no technical skills from the curators. AssetCollector has been successfully applied for organizing various collections of cultural assets in Greece, such as archaeological sites, museums and published books. In the future, an evaluation procedure is planned in order to further refine the use of the system according to the targeted users’ needs. Furthermore, more import and export facilities will be provided, which will make the system compliant with widely accepted standards.     

A quantitative analysis of the European Automakers’ voluntary commitment to reduce CO2 emissions from new passenger cars based on independent experimental data

The reduction of CO₂ emissions and fuel consumption from road transportation constitutes an important pillar of the European Union strategy for implementing the Kyoto Protocol. The commitment to reduce passenger car average CO₂ emissions at 140 g/km in 2008 signed by European car manufacturers and the European Commission is up to now the most important initiative towards limiting CO₂ emissions from road transportation and particularly from passenger cars. Until today, annual reports show the manufacturers’ efforts in limiting CO₂ emissions is within the intermediate target set by the commitment and these results are incorporated in emissions estimations and scientific studies. This paper analyses the origin of the progress achieved so far in CO₂ emissions and attempts an assessment of the commitment using independent experimental emission data. Additionally, the applicability of the commitment-monitoring data into policy and decision-making tools is being examined. The results indicate that a significant part of the reductions achieved so far is due to a market shift towards diesel vehicle sales and that no reduction factors should be applied yet in CO₂ emissions estimation models.     

Performance analysis of IEEE 802.11 ad hoc networks in the presence of exposed terminals

The paper evaluates the performance effects of exposed terminals in IEEE 802.11 ad hoc networks in finite load conditions. It derives analytical models for the estimation of channel utilization and media access delay for IEEE 802.11 ad hoc networks in finite load conditions with and without exposed terminals. The simulation results show that the analytical estimated channel utilization and media access delay metrics are fairly accurate.     

中国充电器标准化带来的变化

中国政府率先开始了手机充电器的标准化,其目的是让用户更换手机终端后可继续使用原来的充电器,从而通过削减废弃物达到保护环境的目的。     

Exergoeconomic analysis and evaluation of energy-conversion plants—I. A new general methodology

A new approach to the combination of exergetic and economic analysis (exergoeconomic analysis) for the investigation of energy-conversion processes is presented. This approach allows the monetary evaluation of costs caused by irreversibilities (exergy losses), as well as comparisons between these costs and the investment and operating costs for each component of a plant. The present analysis permits identification and evaluation of inefficiencies in an energy-conversion plant and of opportunities for improvement.     

New amino acid polymorphism, Ala/Val4058, in exon 45 of the polycystic kidney disease 1 gene: evolution of alleles

The PKD1 gene, which is responsible for the most common form of autosomal dominant polycystic kidney disease, has recently been cloned and sequenced. Many disease-causing mutations have been characterized in this gene, most of them resulting in premature protein termination. However, mutation analysis not routinely implemented for family investigations in a clinical setting, because of the large size and complexity of the gene. Instead, genetic linkage analysis using highly polymorphic CA dinucleotide repeats that map around the gene is still the method of choice. Recently, a few intragenic polymorphisms have been described that are also useful for linkage studies. Here, a new diallelic polymorphism is described for amino acid residue 4058, Ala/Val4058, with allelic frequencies of 0.88 and 0.12, respectively, and a heterozygosity of 0.23, in the Greek and Greek-Cypriot populations. Interestingly, this polymorphism and Ala4091-A/G, which has previously been described in Caucasians, were not detected in DNA from 44 Japanese samples tested. This is particularly important when allelic frequencies in a particular population are used for linkage analysis of families of different ethnic origin. Also, observation of the two polymorphisms together as haplotypes suggests that the Ala/Val4058 polymorphism occurred more recently than the establishment of the Ala4091-A/G polymorphism, and specifically on the G allele.