Synthesis,characterisation and flame,thermal and electrical properties of poly (<Emphasis Type="Italic">n</Emphasis>-butyl methacrylate)/titanium dioxide nanocomposites

Nanocomposites based on poly (n-butyl methacrylate) (PBMA) with various concentrations of titanium dioxide (TiO₂) nanoparticles were synthesised by in situ free radical polymerisation method. The formation of nanocomposite was characterised by FTIR, UV, XRD, DSC, TGA, impedance analyser and flame retardancy measurements. FTIR and UV spectrum ascertained the intermolecular interaction between nanoparticles and the polymer chain. The XRD studies indicated that the amorphous region of PBMA decreased with the increase in content of metal oxide nanoparticles. The SEM revealed the uniform dispersion of nanoparticles in the polymer composite. The DSC and TGA studies showed that the glass transition temperature and thermal stability of the nanocomposites were increased with the increase in the concentration of nanoparticles. The conductivity and dielectric properties of nanocomposites were higher than pure PBMA and the maximum electrical property was observed for the sample with 7 wt% TiO₂. As the concentration of nanoparticles increased above 7 wt%, the electrical property of nanocomposite was decreased owing to the agglomeration of nanoparticles in the polymer. Nanoparticles could impart better flame retardancy to PBMA/TiO₂ composite and the flame resistance of the materials improved with the addition of nanoparticles in the polymer matrix.     

An evaluation of the effectiveness of forward facing child restraint systems

The objective of this study was to determine the effectiveness of forward facing child restraint systems (FFCRS) in preventing serious injury and hospitalization to children 12-47 months of age as compared with similar age children in seat belts. Data were obtained from a cross-sectional study of children aged 12-47 months in crashes of insured vehicles in 15 states, with data collected via insurance claims records and a telephone survey. Effectiveness estimates were limited to those children between 12 and 47 months of age seated in the back row(s) of vehicles, restrained in FFCRS, regardless of misuse, or seat belts of all types and usage. Completed survey information was obtained on 1207 children, representing 12632 children in 11619 crashes between 1 December 1998 and 31 May 2002. Serious injuries occurred to 0.47% of all 12-47-month olds studied, including 1.72% of those in seat belts and 0.39% of those in child restraint systems. The risk of serious injury was 78% lower for children in FFCRS than in seat belts (odds ratio (OR) = 0.22, 95% confidence interval (CI) = 0.11-0.45, P = 0.001). The risk of hospitalization was 79% lower for children in FFCRS than in seat belts (OR = 0.21, 95% CI = 0.09-050, P = 0.001). There was no difference between the restraint types in preventing minor injuries. As compared with seat belts, CRS are very highly effective in preventing serious injuries and hospitalization, respectively. This effectiveness estimate is substantially higher than older estimates, demonstrating the benefits of current CRS designs. These results provide those educating parents and caregivers population-based data on the importance of child restraint use.     

T-cell epitope analysis using subtracted expression libraries (TEASEL): application to a 38-kDA autoantigen recognized by T cells from an insulin-dependent diabetic patient

Studies on circulating T cells and antibodies in newly diagnosed type 1 diabetic patients and rodent models of autoimmune diabetes suggest that beta-cell membrane proteins of 38 kDa may be important molecular targets of autoimmune attack. Biochemical approaches to the isolation and identification of the 38-kDa autoantigen have been hampered by the restricted availability of islet tissue and the low abundance of the protein. A procedure of epitope analysis for CD4+ T cells using subtracted expression libraries (TEASEL) was developed and used to clone a 70-amino acid pancreatic beta-cell peptide incorporating an epitope recognized by a 38-kDa-reactive CD4+ T-cell clone (1C6) isolated from a human diabetic patient. The minimal epitope was mapped to a 10-amino acid synthetic peptide containing a DR1 consensus binding motif. Data base searches did not reveal the identity of the protein, though a weak homology to the bacterial superantigens SEA (Streptococcus pyogenes exotoxin A) and SEB (Staphylococcus aureus enterotoxin B) (23% identity) was evident. The TEASEL procedure might be used to identify epitopes of other autoantigens recognized by CD4+ T cells in diabetes as well as be more generally applicable to the study low-abundance autoantigens in other tissue-specific autoimmune diseases.     

Short-term physical limitations in children following motor vehicle crashes

This study describes frequency of injury and short-term physical limitation among child occupants ≤15 years in motor vehicle crashes and examines the association between age, restraint use, seating position, and type of crash on the presence of physical limitations. Conducted from 1/1/2005–11/30/2007, as part of a child-specific crash surveillance system in 15 U.S. states; data were collected using claims records and parent/driver telephone surveys. Respondents were asked whether children sustained physical limitations from the crash and the duration limitations persisted. Overall, 3.3% had ≥1 physical limitations. Limitations increased with age, from 0.7% for children ≤3 years to 7.6% for adolescents 13–15 years (p < 0.001). Among children with AIS ≥2 injuries, the proportion with physical limitations ranged from 58% to 91% depending on injury diagnosis. Among children with whiplash, 47% resulted in physical limitations. Suboptimally restrained children were nearly twice as likely to have a limitation compared to optimally restrained children. After adjusting for driver characteristics and vehicle type, child's age, restraint use, and type of initial impact were independently associated with the presence of physical limitations. Our results show the importance of assessing children for physical limitations following motor vehicle crashes. We also observed that children with whiplash were at risk for physical limitations.     

Seat belt use among 13-15 year olds in primary and secondary enforcement law states

CONTEXT: Coverage and enforcement provisions of safety belt use laws vary by state. Most laws cover drivers and passengers age 16 and above. "Primary" enforcement laws allow police to stop and ticket a motorist for a safety belt law violation. With "secondary" enforcement laws, ticketing can only occur in the presence of another traffic infraction. Given the lower rates of restraint use among teen drivers and their passengers, the effect of primary enforcement laws on the restraint use of young teen passengers is of particular interest. OBJECTIVE: To compare restraint use by 13-15 year olds passengers in motor vehicle crashes in states with primary versus secondary enforcement safety belt laws. METHODS: Cross-sectional study of 3953 crashes involving 5372 children, representing 54,226 children aged 13-15 years in 41,198 crashes of insured vehicles in 16 states and Washington, DC between December 1, 1998 and December 31, 2004, with data collected via insurance claims records and a telephone survey. RESULTS: Non-use of restraints by 13-15 year olds was 7.2% (95% CI 4.3-10.1%) greater in secondary enforcement states (10.8%) as compared to states with primary enforcement laws (3.6%). After controlling for driver's age and restraint status and the seating row of the occupant, a 13-15 year olds was over twice as likely to be unrestrained in a secondary enforcement state as compared to a primary enforcement state (adjusted RR=2.2, 95% CI 1.5-3.1). CONCLUSIONS: States considering primary enforcement provisions to their restraint laws should consider the potential benefits these laws may have to all occupants in the vehicle, particularly young adolescents who are nearing driving age.     

Effect of neodymium‐doped titanium dioxide nanoparticles on the structural,mechanical, and electrical properties of poly(butyl methacrylate) nanocomposites

Nanocomposites based on neodymium‐doped titanium dioxide (Nd‐TiO₂)/poly(n‐butyl methacrylate) (PBMA) have been prepared by an in situ polymerization of butyl methacrylate monomer with varying concentrations of Nd‐TiO₂ nanoparticles. The resulting nanocomposites have been analyzed by ultraviolet (UV)–Visible spectroscopy, Fourier‐transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscope (SEM), differential scanning calorimetry (DSC), thermogravimetric analysis, and impedance analyzer (TGA). The results of UV and FTIR spectroscopy have indicated the interaction of nanoparticles with the PBMA matrix. Spherically shaped nanoparticles with an average size of 10–25 nm have been revealed in the TEM and their homogeneous dispersion, and interaction of polymer matrix has been confirmed by SEM and XRD studies. The thermal stability and glass transition temperature of the composites were significantly enhanced by the addition of nanoparticles. The AC conductivity and dielectric properties of nanocomposites have been found to be higher than pure PBMA, and the maximum electrical properties have been observed for 7 wt% composite. The reinforcing nature of the nanoparticles in PBMA has been reflected in the improvement in tensile strength measurements. The result indicated that the tensile strength of nanocomposites have greatly enhanced by the addition of Nd‐TiO₂ nanoparticles whereas the elongation at break decreases with the loading of nanofillers. To understand the mechanism of reinforcement, tensile strength values have been correlated with various theoretical modeling. The research has been found to be promising in the development of novel materials with enhanced tensile strength, dielectric constant, and thermal properties, which may find potential applications in energy storage and nanoelectronic devices. J. VINYL ADDIT. TECHNOL., 25:9–18, 2019. © 2018 Society of Plastics Engineers     

Yohimbine, erectile capacity, and sexual response in men

In this study we investigated the hypothesis that cartilage from defined regions of ovine stifle joints, which were subjected to differing mechanical stresses, contained phenotypically distinct chondrocyte populations. Chondrocyte phenotypes were identified by the relative biosynthesis of the proteoglycans (PGs) aggrecan, biglycan and decorin. Articular cartilage (AC) from adult and neonatal ovine stifle joints were examined. Cells were cultured as both full-depth AC explants and in alginate beads after their isolation from the AC matrix. When chondrocytes from the various topographical regions of adult ovine knee joints were cultured as explants they demonstrated a consistent difference with regard to the metabolism of aggrecan and decorin. Significantly, this topographically-dependent phenotypic expression of PGs was preserved when the chondrocytes were cultured in alginate beads. In adult joints, chondrocytes from the central region of the tibial plateau not covered by the meniscus, which is subjected to high mechanical loads in-vivo, synthesized less aggrecan but more decorin than cells from regions covered by the meniscus. When chondrocytes from identical AC regions of neonatal ovine joints were cultured as explants, no topographical difference in aggrecan nor decorin metabolism could be detected. The results of this study, in association with the existing literature, lead us to propose that post-natal mechanical loading of AC could select for chondrocyte clones or induce a lasting modulation of chondrocyte phenotypic expression in different joint regions. Such cellular changes could result in the synthesis of PG populations that confer properties to AC most suited to resist the variable mechanical stresses in the different joint regions. This study serves to emphasize the importance of using cartilage from identical joint areas when examining PG metabolism by chondrocytes. Further investigation into the relationship between mechanical loading, regional chondrocyte phenotype selection and the response of these cells to anabolic and catabolic factors may provide important insights into the focal nature of AC degeneration in osteoarthritis.     

Th1-like cytokine production profile and individual specific alterations in TCRBV-gene usage of T cells from newly diagnosed type 1 diabetes patients after stimulation with beta-cell antigens

Thirty complete coding sequences of human major histocompatibility complex (Mhc) class II DRB alleles, spanning 237 codons, were analyzed for phylogenetic information using distance, parsimony, and likelihood approaches. Allelic genealogies derived from different parts of the coding sequence (exon 2, the 5' and 3' ends of exon 2, respectively, and exons 3-6) were compared. Contrary to prior assertions, a rigorous analysis of allelic genealogies in this gene family cannot be used to justify the claim that the lineage leading to modern humans contained on average at least 100,000 individuals. Phylogenetic inferences based upon the exon 2 region of the DRB loci are complicated by selection and recombination, so this part of the gene does not provide a complete and accurate view of allelic relationships. Attempts to reconstruct human history from genetic data must use realistic models which consider the complicating factors of nonequilibrium populations, recombination, and different patterns of selection.     

Effect of Nano-Ce-Doped TiO<Subscript>2</Subscript> on AC Conductivity and DC Conductivity Modeling Studies of Poly (<Emphasis Type="Italic">n</Emphasis>-Butyl Methacrylate)

Among the unique properties of polymer nanocomposites, electrical conductivity deserves a prominent place due to their wide applications in conducting adhesive, electromagnetic shielding and sensors. The present work focuses on the effect of cerium-doped titanium dioxide (Ce-TiO₂) nanoparticles on the conductivity studies of poly (n-butyl methacrylate), or PBMA, nanocomposites at different temperatures. The frequency-dependent alternating current (AC) electrical conductivity of PBMA/Ce-TiO₂ nanocomposites has been found to increase with increase in temperature and the concentration of Ce-TiO₂ nanoparticles. The activation energy calculated from the AC electrical conductivity has been found to decrease with frequency and increasing temperatures. The frequency exponent factor also showed a decrease with frequency, indicating the hopping conduction in the nanocomposites. The maximum AC conductivity has been observed for the composites with 7 wt.% sample. The direct current (DC) conductivity of PBMA/Ce-TiO₂ composites was also enhanced with the addition of Ce-TiO₂ nanoparticles. Experimental and theoretical investigations based on Scarisbrick, Bueche, McCullough and Mamunya modeling were undertaken to understand the observed DC conductivity differences induced by the addition of Ce-doped TiO₂ nanoparticles to PBMA matrix. The experimental conductivity showed good agreement with the theoretical conductivity observed using the Mamunya model.