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1.
Olga V. Kovaleva Madina A. Rashidova Daria V. Samoilova Polina A. Podlesnaya Rasul M. Tabiev Valeria V. Mochalnikova Alexei Gratchev 《International journal of molecular sciences》2021,22(1)
There is an urgent need for identification of new prognostic markers and therapeutic targets for non-small cell lung cancer (NSCLC). In this study, we evaluated immune cells markers in 100 NSCLC specimens. Immunohistochemical analysis revealed no prognostic value for the markers studied, except CD163 and CD206. At the same time, macrophage markers iNOS and CHID1 were found to be expressed in tumor cells and associated with prognosis. We showed that high iNOS expression is a marker of favorable prognosis for squamous cell lung carcinoma (SCC), and NSCLC in general. Similarly, high CHID1 expression is a marker of good prognosis in adenocarcinoma and in NSCLC in general. Analysis of prognostic significance of a high CHID1/iNOS expression combination showed favorable prognosis with 20 months overall survival of patients from the low CHID1/iNOS expression group. For the first time, we demonstrated that CHID1 can be expressed by NSCLC cells and its high expression is a marker of good prognosis for adenocarcinoma and NSCLC in general. At the same time, high expression of iNOS in tumor cells is a marker of good prognosis in SCC. When used in combination, CHID1 and iNOS show a very good prognostic capacity for NSCLC. We suggest that in the case of lung cancer, tumor-associated macrophages are likely ineffective as a therapeutic target. At the same time, macrophage markers expressed by tumor cells may be considered as targets for anti-tumor therapy or, as in the case of CHID1, as potential anti-tumor agents. 相似文献
2.
Md Saifur Rahman Md Badrul Alam Young Kyun Kim Mst Hur Madina Ismail Fliss Sang Han Lee Jin Cheol Yoo 《International journal of molecular sciences》2021,22(10)
In this study, we investigate the immunomodulatory effects of a novel antimicrobial peptide, YD1, isolated from Kimchi, in both in vitro and in vivo models. We establish that YD1 exerts its anti-inflammatory effects via up-regulation of the Nrf2 pathway, resulting in the production of HO-1, which suppresses activation of the NF-κB pathway, including the subsequent proinflammatory cytokines IL-1β, IL-6, and TNF-α. We also found that YD1 robustly suppresses nitric oxide (NO) and prostaglandin E2 (PGE2) production by down-regulating the expression of the upstream genes, iNOS and COX-2, acting as a strong antioxidant. Collectively, YD1 exhibits vigorous anti-inflammatory and antioxidant activity, presenting it as an interesting potential therapeutic agent. 相似文献
3.
Dr. Madina Mansurova Julian Simon Dr. Susanne Salzmann Prof. Dr. Christel M. Marian Prof. Dr. Wolfgang Gärtner 《Chembiochem : a European journal of chemical biology》2013,14(5):645-654
Two chemically synthesized flavin derivatives, 8‐trifluoromethyl‐ and 8‐bromoriboflavin (8‐CF3RF and 8‐BrRF), were photochemically characterized in H2O and studied spectroscopically after incorporation into the LOV domain of the blue light photoreceptor YtvA from Bacillus subtilis. The spectroscopic studies were paralleled by high‐level quantum chemical calculations. In solution, 8‐BrRF showed a remarkably high triplet quantum yield (0.97, parent compound riboflavin, RF: 0.6) and a small fluorescence quantum yield (0.07, RF: 0.27). For 8‐CF3RF, the triplet yield was 0.12, and the fluorescence quantum yield was 0.7. The high triplet yield of 8‐BrRF is due to the bromine heavy atom effect causing a stronger spin–orbit coupling. Theoretical calculations reveal that the decreased triplet yield of 8‐CF3RF is due to a smaller charge transfer and a less favorable energetic position of T2, required for intersystem crossing from S1 to T1, as an effect of the electron‐withdrawing CF3 group. The reconstitution of the LOV domain with the new flavins resulted in the typical LOV photochemistry, consisting of triplet state formation and covalent binding of the chromophore, followed by a thermal recovery of the parent state, albeit with different kinetics and photophysical properties. 相似文献
4.
The calculation of the degree d of an approximate greatest common divisor of two inexact polynomials f(y) and g(y) reduces to the determination of the rank loss of a resultant matrix, the entries of which are functions of the coefficients of f(y) and g(y). This paper considers this issue by describing two methods to calculate d, such that knowledge of the noise level imposed on the coefficients of f(y) and g(y) is not assumed. One method uses the residual of a linear algebraic equation whose coefficient matrix and right hand side vector are derived from the Sylvester resultant matrix S(f,g), and the other method uses the first principal angle between a line and a hyperplane, the equations of which are calculated from S(f,g). Computational results on inexact polynomials whose exact forms have multiple roots of high degree are shown and very good results are obtained. These results are compared with the rank loss of S(f,g) for the calculation of d, and it is shown that this method yields incorrect results for these examples. 相似文献
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6.
Ilmutdin M. Abdulagatov Vasilii I. Dvoryanchikov Abdurakhman N. Kamalov Elena G. Abramova Madina A. Abdurashidova 《加拿大化工杂志》2000,78(3):547-556
Isochoric heat capacities of H2O+NaCl solutions (0.0031 and 0.0063 mole fraction of NaCl) were measured with a high temperature and high pressure adiabatic calorimeter near the sub‐critical and near the supercritical point of pure water. Temperatures ranged from 361 to 677 K. Measurements were made at six densities, namely: 367.07 and 485.59 kg·m?3 for x = 0.0031 mol fraction of NaCl and 388.65, 560.03, 607.05, and 973.70 kg·m?3 for x = 0.0063 mol fraction of NaCl. Measurements were conducted in the two‐and one‐phase regions including near phase transition temperatures TS(ρ). The phase transition temperatures TS(ρ) and saturated isochoric heat capacity values CVX have been determined for each isochore. The uncertainty in heat capacity measurements is estimated to be 3.5% to 4.5% near the phase transition and critical points. Present and previous results of heat capacity measurements were compared with predictions from the crossover (CREOS) and Pitzer—Tanger–Hovey equations of state (PTH EOS). Our previous heat capacity measurements were found to deviate systematically from crossover‐model predictions. The present results show good agreement with the crossover model for the composition x = 0.0031 mol fraction. 相似文献
7.
Chieko Otsubo Zhanna Mussazhanova Hirokazu Kurohama Gulzira Shalgimbayeva Nozomi Ueki Yuki Matsuoka Madina Madiyeva Shinya Sato Hiroyuki Yamashita Masahiro Nakashima 《International journal of molecular sciences》2023,24(1)
Nodal metastasis is crucial for determining the stage of well-differentiated thyroid cancer (WTC) in patients older than 55. Well-formed thyroid follicular inclusions (TFIs) are occasionally encountered in the cervical lymph nodes (LNs) of patients with papillary thyroid carcinoma (PTC), and it is difficult to determine whether they are true nodal metastases or ectopic thyroid tissues (ETT). This study aimed to elucidate the impact of the expression of the DNA damage response molecule TP53-binding protein 1 (53BP1) using immunofluorescence (IF) as a biomarker to differentiate TFIs in cervical LN by comparing the mutation analyses of primary thyroid cancers. The data demonstrated the necessity for the differential diagnosis of true metastases from ETT among TFIs in cervical LNs. PTC-like nuclear features using hematoxylin–eosin staining combined with immunohistochemistry for conventional biomarkers of PTC, including BRAFV600E protein, were most helpful in identifying metastatic follicular-patterned carcinomas. In conclusion, IF analysis of 53BP1 expression could be an excellent ancillary technique to distinguish metastatic carcinoma or ETT from TFIs in LNs, particularly in cases other than BRAFV600E-mutated PTC. 相似文献
8.
Compatibility of materials with hydrogen. Particular case: Hydrogen embrittlement of titanium alloys
A review of the effect of hydrogen on materials is addressed in this paper. General aspects of the interaction of hydrogen and materials, hydrogen embrittlement, low temperature effects, material suitability for hydrogen service and materials testing are the main subjects considered in the first part of the paper. As a particular case of the effect of hydrogen in materials, the hydride formation of titanium alloys is considered. Hydrogen absorption and the possible associated problems must be taken into account when considering titanium as a candidate material for high responsibility applications. The sensitivity of three different titanium alloys to the Hydrogen Assisted Stress Cracking phenomena has been studied by means of the Slow Strain Rate Technique (SSRT). The testing media have been sea water and hydrogen has been produced on the specimen surface during the test by cathodic polarization. Tested specimens have been characterized by metallography and scanning electron microscopy. Results obtained show that the microstructure of the materials, particularly the β-phase content, plays an important role on the sensitivity of the studied alloys to the Hydrogen Assisted Stress Cracking Phenomena. 相似文献
9.
Ivan V. Mikheev Madina M. Sozarukova Dmitry Yu. Izmailov Ivan E. Kareev Elena V. Proskurnina Mikhail A. Proskurnin 《International journal of molecular sciences》2021,22(11)
The antioxidant potential (capacity and activity) of aqueous fullerene dispersions (AFD) of non-functionalized C60, C70, and Gd@C82 endofullerene (in micromolar concentration range) was estimated based on chemiluminescence measurements of the model of luminol and generation of organic radicals by 2,2′-azobis(2-amidinopropane) dihydrochloride (ABAP). The antioxidant capacity was estimated by the TRAP method, from the concentration of half-suppression, and from the suppression area in the initial period. All three approaches agree and show that the antioxidant capacity of AFDs increased in the order Gd@C82 < C70 < C60. Mathematical modeling of the long-term kinetics data was used for antioxidant activity estimation. The effect of C60 and C70 is found to be quenching of the excited product of luminol with ABAP-generated radical and not an actual antioxidant effect; quenching constants differ insignificantly. Apart from quenching with a similar constant, the AFD of Gd@C82 exhibits actual antioxidant action. The antioxidant activity in Gd@C82 is 300-fold higher than quenching constants. 相似文献
10.
Oscar Saz Salil Deena Mortaza Doulaty Madina Hasan Bilal Khaliq Rosanna Milner Raymond W. M. Ng Julia Olcoz Thomas Hain 《Multimedia Tools and Applications》2018,77(23):30533-30550
This paper describes a system for performing alignment of subtitles to audio on multigenre broadcasts using a lightly supervised approach. Accurate alignment of subtitles plays a substantial role in the daily work of media companies and currently still requires large human effort. Here, a comprehensive approach to performing this task in an automated way using lightly supervised alignment is proposed. The paper explores the different alternatives to speech segmentation, lightly supervised speech recognition and alignment of text streams. The proposed system uses lightly supervised decoding to improve the alignment accuracy by performing language model adaptation using the target subtitles. The system thus built achieves the third best reported result in the alignment of broadcast subtitles in the Multi–Genre Broadcast (MGB) challenge, with an F1 score of 88.8%. This system is available for research and other non–commercial purposes through webASR, the University of Sheffield’s cloud–based speech technology web service. Taking as inputs an audio file and untimed subtitles, webASR can produce timed subtitles in multiple formats, including TTML, WebVTT and SRT. 相似文献